Identification, expression profiling and localization of thoc in common carp ovary: Influence of thoc3-siRNA transient silencing.

07:00 EST 11th January 2020 | BioPortfolio

Summary of "Identification, expression profiling and localization of thoc in common carp ovary: Influence of thoc3-siRNA transient silencing."

The THO complex is a multisubunit complex with a function in transcription and mRNA export. In the present study, THO complex transcripts (thoc) were identified in developing ovary of common carp and their role during ovarian development and growth has been characterized for the first time in a teleost using expression profiling and transient siRNA silencing. Thoc expression revealed a spatiotemporal pattern in the gonads with high levels at 120 days post-hatch, with moderately high levels thereafter. In situ hybridization and immunohistochemical localization revealed the presence of thoc3 in follicular layer of stage-III/IV oocytes. High levels of thoc3, thoc5, and thoc7 genes in the follicular layer suggest a possible role in ovarian growth. Reduced levels of serum estradiol-17β and 17α,20β-dihydroxypregn-4-en-3-one after thoc3 transient silencing indicated differential action on steroidogenic enzyme, transcription factor, and growth factor genes. Furthermore, transient silencing of thoc3, in vivo and in vitro, downregulated ad4bp/sf1, amh, cyp19a1a, foxl2, hsd3b, hsd11b1, hsd20b, hsd17b1, rspo1, and vtg. Incidentally, gdf9 and igf1 were upregulated, while no change was seen in esr1/2, nanos, and vasa. These observations imply that thoc3 seems to regulate ovarian function including steroidogenesis, either directly or indirectly.


Journal Details

This article was published in the following journal.

Name: Gene
ISSN: 1879-0038
Pages: 144350


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