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A Rapid Survey of Avirulence Genes in Field Isolates of .

07:00 EST 13th January 2020 | BioPortfolio

Summary of "A Rapid Survey of Avirulence Genes in Field Isolates of ."

is the causal agent for the devastating disease rice blast. The avirulence () genes in are required to initiate robust disease resistance mediated by the corresponding resistance () genes in rice. Therefore, monitoring pathogen genes is important to predict the stability of gene-mediated blast resistance. In the present study, we analyzed the DNA sequence dynamics of five genes, namely, , , , , and , in field isolates of in order to understand the effectiveness of the genes, , , , , and in the Southern U.S. rice growing region. Genomic DNA of 258 blast isolates collected from commercial fields of the Southern UNITED STATES during 1975-2009 were subjected to PCR amplification with gene-specific PCR markers. PCR products were obtained from 232 isolates. The absence of PCR products in the remaining 26 isolates suggests that these isolates do not contain the tested genes. Amplified PCR products were subsequently gel purified and sequenced. Based on the presence or absence of the five genes, 232 field isolates were classified into 10 haplotype groups. The results revealed that 174 isolates of carried , 225 isolates carried , 44 isolates carried , 3 isolates carried , and one isolate carried . was highly variable, and 40 haplotypes were identified in avirulent isolates. had four nucleotide sequence site changes resulting in amino acid substitutions, whereas three other genes, , , and , were relatively stable. Two genes, and , were found to exist in relatively larger proportions of the tested field isolates, which suggested that their corresponding genes and can be deployed in preventing blast disease in the Southern UNITED STATES in addition to . This study demonstrates that continued gene monitoring in the pathogen population is critical for ensuring the effectiveness of deployed blast genes in commercial rice fields.

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This article was published in the following journal.

Name: Plant disease
ISSN: 0191-2917
Pages: PDIS08191688RE

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