Saline-indirect antiglobulin test.

07:00 EST 1st December 2019 | BioPortfolio

Summary of "Saline-indirect antiglobulin test."

A saline-indirect antiglobulin test (IAT) is performed without addition of enhancement media to increase the binding of antibody to the red blood cell antigen during the 37°C incubation. Although infrequently used as a primary means for antibody detection or identification, this test is useful because of the variety of possible applications in antibody identification studies. It is critical to the test sensitivity to allow enough incubation time (30-60 minutes) for maximum antibody binding to occur. The saline test can also be subject to a direct agglutination reading after immediate spin, room temperature, or incubation at 37°C before conversion to the IAT. This step allows further flexibility in assessing the reactivity of directly agglutinating allo- or autoantibodies in tests performed at 37°C or lower temperatures.


Journal Details

This article was published in the following journal.

Name: Immunohematology
ISSN: 0894-203X
Pages: 156-158


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Medical and Biotech [MESH] Definitions

Hemagglutination test in which Coombs' reagent (antiglobulin, or anti-human globulin rabbit immune serum) is added to detect incomplete (non-agglutinating, univalent, blocking) antibodies coating erythrocytes. The direct test is applied to red cells which have been coated with antibody in vivo (e.g., in hemolytic disease of newborn, autoimmune hemolytic anemia, and transfusion reactions). The indirect test is applied to serum to detect the presence of antibody (e.g., in detection of incompatibility in cross-matching tests, detection and identification of irregular antibodies, and in detection of antibodies not identifiable by other means).

A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.

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A genus of HALOBACTERIACEAE distinguished from other genera in the family by the presence of specific derivatives of TGD-2 polar lipids. Haloarcula are found in neutral saline environments such as salt lakes, marine salterns, and saline soils.

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