Track topics on Twitter Track topics that are important to you
Trypsin is an enzyme first discovered in the quest to increase the detection of newly found Rh antibodies. Because of the crude source of trypsin and challenges in its consistency in test conditions, additional enzymes and chemical treatments were devised as alternative sources to enhance the detection of antibodies. The key to successful trypsin treatment starts with reagent preparation. Optimal testing conditions should be determined with each batch of trypsin prepared. As in all enzyme treatments, quality control is required to ensure proper removal of the expected antigen(s) while not producing enzyme-specific panagglutination. Once successful quality control results have been obtained, trypsin treatment can be used to rule out "common" clinically significant alloantibodies and provide direction in the identification of antibodies to high-prevalence antigens. Trypsin treatment is a key player in the Immunohematology Reference Laboratory but is often overlooked as a tool in the Transfusion Service. If performed using the proper preparation and quality control, trypsin treatment can be a valuable tool in the serology toolkit in both settings.
This article was published in the following journal.
Trypsin purified from the spleen of albacore tuna was immobilized onto Octyl Sepharose CL-4B, glutaraldehyde activated silica and 5'-4,4'-dimethyltryptamine-thymidine-succinyl controlled pore glass. T...
In this work, the effects of I on the activities and conformational structures of digestive enzymes, trypsin and pepsin were studied. The results indicated that the enzyme activities were decreased to...
Trypsin is by far the most commonly used protease in proteomics. Even though the amount of protease used in each experiment is very small, digestion of large amounts of protein prior to enrichment can...
The structural studies of trypsin with curcumin in Tris-hydrochloride (Tris-HCl) buffer solution (pH 8.0) was explored by UV-vis spectroscopic and fluorescence quenching method, kinetic reaction, circ...
Lyme disease is a tickborne zoonosis for which serologic testing is the principal means of laboratory diagnosis. In 1994, the Association of State and Territorial Public Health Laboratory Directors, C...
Siblings of those with type 1 diabetes are at an increased risk of developing the disease themselves. Through prior research, the investigators have found that siblings as well as those wi...
The purpose of this study is to collect serum samples to evaluate serologic assays and to establish proficiency panels for serologic assays used for assessment of post vaccination immune r...
The objective of the study is to evaluate the acceptance and effect of type- specific HSV serologic testing of pregnant women on sexual behavior at the end of pregnancy.
The purposes of this study are: - To determine the accuracy of commercially available serologic assays in diagnosing patients with herpes simplex virus type 2 (HSV-2); - ...
Earlier protocol for cultivated oral mucosal epithelial transplantation (COMET) requires trypsin/EDTA to isolate epithelial cells from tissue, and uses murine 3T3 cells as feeder cells, wh...
A pancreatic trypsin inhibitor common to all mammals. It is secreted with the zymogens into the pancreatic juice. It is a protein composed of 56 amino acid residues and is different in amino acid composition and physiological activity from the Kunitz bovine pancreatic trypsin inhibitor (APROTININ).
Serine proteinase inhibitors which inhibit trypsin. They may be endogenous or exogenous compounds.
A high-molecular-weight protein (approximately 22,500) containing 198 amino acid residues. It is a strong inhibitor of trypsin and human plasmin.
A family of trypsin-like SERINE ENDOPEPTIDASES that are expressed in a variety of cell types including human prostate epithelial cells. They are formed from tissue prokallikrein by action with TRYPSIN. They are highly similar to PROSTATE-SPECIFIC ANTIGEN. EC 22.214.171.124.
A low-molecular-weight protein (minimum molecular weight 8000) which has the ability to inhibit trypsin as well as chymotrypsin at independent binding sites. It is characterized by a high cystine content and the absence of glycine.
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...
An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens. Examples of antigens include microorganisms (such as bacteria, fungi, parasites, and viruses) and chemicals. Antibodies may be produc...