Inflammatory biomarkers in the blood and pancreatic tissue of organ donors that predict human islet isolation success and function.

07:00 EST 14th January 2020 | BioPortfolio

Summary of "Inflammatory biomarkers in the blood and pancreatic tissue of organ donors that predict human islet isolation success and function."

The pancreas of brain-dead donors is the primary source of islets for transplantation. However, brain death mediates systemic inflammation, which may affect the quantity and quality of isolated islets. Our aim was to identify inflammatory biomarkers in donor blood and/or pancreatic tissue capable of predicting islet isolation success. Blood samples were collected from 21 pancreas donors and 14 healthy volunteers. Pancreatic tissue samples were also collected from the corresponding donor during organ procurement. Six serum cytokines were measured by a fluorescent bead-based immunoassay, and the expression of fifteen inflammatory target genes was quantified by quantitative reverse transcription polymerase chain reaction (RT-qPCR). There was no correlation between serum inflammatory cytokines and mRNA expression of the corresponding genes in peripheral blood mononuclear cells (PBMCs) or pancreatic tissue. The expression in pancreatic tissue correlated negatively with post-isolation islet yield. Islets isolated from donors highly expressing in PBMCs and in pancreatic tissue functioned poorly when transplanted in diabetic NOD mice. Furthermore, the increased in pancreatic tissue was positively correlated with donor hospitalization time. Brain death duration positively correlated with higher expression of in PBMCs and in both PBMCs and pancreatic tissue but failed to show a significant correlation with islet yield and function. The study indicates that the increased inflammatory genes in donor pancreatic tissues may be considered as biomarkers associated with poor islet isolation outcome.


Journal Details

This article was published in the following journal.

Name: Islets
ISSN: 1938-2022
Pages: 1-11


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Medical and Biotech [MESH] Definitions

The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.

The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation.

Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.

The chilling of a tissue or organ during decreased BLOOD perfusion or in the absence of blood supply. Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN PROCUREMENT surgery, and ends after the tissue reaches physiological temperature during implantation procedures. WARM ISCHEMIA TIME starts then and ends with completion of SURGICAL ANASTOMOSIS.

A tissue or organ remaining at physiological temperature during decreased BLOOD perfusion or in the absence of blood supply. During ORGAN TRANSPLANTATION it begins when the organ reaches physiological temperature before the completion of SURGICAL ANASTOMOSIS and ends with reestablishment of the BLOOD CIRCULATION through the tissue.

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