Production of antifungal saponins in an airlift bioreactor with a cell line transformed from and its activity against strawberry phytopathogens.

07:00 EST 14th January 2020 | BioPortfolio

Summary of "Production of antifungal saponins in an airlift bioreactor with a cell line transformed from and its activity against strawberry phytopathogens."

Biotechnology through plant cell cultures in bioreactors is a tool that allows increasing the production of secondary metabolites of commercial interest. The hydrodynamic characterization, in addition to the transfer (OTR) and uptake (OUR) of oxygen through the dynamic method with different aeration rate, were used to see their influence on the production of biomass and saponins. The culture poisoning technique was used to determine the antifungal activity of the SC-2 and SC-3 saponins . Likewise, the shear or hydrodynamic stress of 273.6 mN/m were calculated based on the Reynolds Number. The oxygen supply (OTR) was always greater than the demand (OUR) for all the aeration rate evaluated. Dry weight values of 8.6 gDW/L and a concentration of 2.7 mg/L and 187.3 mg/L of the saponins SC-2 and SC-3 respectively were obtained with an air flow of 0.1 vvm. In addition, it was possible to inhibit the growth of phytopathogenic fungi by up to 93%, while it was possible to reduce the infections of strawberry seeds inoculated with phytopathogens, obtaining up to 94% of germinated seeds. This information will facilitate the rational operation of the bioreactor culture system that produces secondary metabolites.


Journal Details

This article was published in the following journal.

Name: Preparative biochemistry & biotechnology
ISSN: 1532-2297
Pages: 1-11


DeepDyve research library

PubMed Articles [22773 Associated PubMed Articles listed on BioPortfolio]

The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor.

To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymi...

Production process development of pseudorabies virus vaccine by using novel scale-down model of fixed-bed bioreactor.

In this study, the novel tube-fixed-bed bioreactor which consist of TubeSpin bioreactor 50 tube and 0.44 g macrocarriers was developed as the scale-down model of fixed-bed bioreactor. The adherent Ver...

The influence of steroidal and triterpenoid saponins on monolayer models of the outer leaflets of human erythrocytes, E. coli and S. cerevisiae cell membranes.

The present paper discusses the use of monolayers of lipid mixtures mimicking the composition of biological membranes of bacteria, erythrocyte and yeast in the context of the anti-bacterial, hemolytic...

Combining Convolutional Neural Networks and In-line Near-infrared Spectroscopy for Real-time Monitoring of the Chromatographic Elution Process in Commercial Production of Notoginseng Total Saponins.

The chromatographic elution process is a key step in the production of notoginseng total saponins. Due to quality variability of loading samples and resin capacity decreasing over cycle time, saponins...

Oleanane-type saponins from the roots of Parkia bicolor A. Chev.

Five undescribed triterpene-type saponins, parkibicolorosides A-E, a cassane-type diterpene, and a known trimethoxy benzene glucoside were isolated from the roots of Parkia bicolor A. Chev. Their stru...

Clinical Trials [11588 Associated Clinical Trials listed on BioPortfolio]

Antifungal Locks to Treat Fungal-Related Central Line Infections

The purpose of this study is to evaluate the usefulness of antifungal lock therapy with liposomal amphotericin B (Ambisome), in combination with systemic antifungal(s), in patients with ca...

Clinical Study on the Treatment of Hypertensive Intracerebral Hemorrhage With Panax Notoginseng Saponin

The purpose of this study is to determine whether panax notoginseng saponins are effective in the treatment of Hypertensive Intracerebral Hemorrhage Patients.

An Efficacy and Safety Study of APX001 in Non-Neutropenic Patients With Candidemia

This is a multicenter, open-label, non-comparative, single-arm study to evaluate the efficacy and safety of APX001 for the first-line treatment for candidemia including suspected or confir...

A Phase III Trial to Assess the Safety and Efficacy of Plant Cell Expressed GCD in Patients With Gaucher Disease

Gaucher disease, the most prevalent lysosomal storage disorder, is caused by mutations in the human glucocerebrosidase gene (GCD) leading to reduced activity of the lysosomal enzyme glucoc...

Heat-clearing and Blood-activating Chinese Medicinal Components in Acute Cerebral Infarction

The present study is designed to confirm the therapeutic effects of heat-clearing and blood-activating Chinese medicinal components in treatment of Acute Cerebral Infarction (ACI) and to i...

Medical and Biotech [MESH] Definitions

A plant genus of the family RHAMNACEAE. Members contain antifungal jujubogenin saponins.

Cell line derived from SF21 CELLS which are a cell line isolated from primary explants of SPODOPTERA FRUGIPERDA pupal tissue.

A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

A cell line derived from cultured tumor cells.

Quick Search

DeepDyve research library

Relevant Topics

Biotechnology - Biotech
"using living things to create products or to do tasks for humans" About Biotechnology - Biotech Biotechnology is the practice of using plants, animals and micro-organisms such as bacteria, as well as biological processes - such as the ripen...

Mental Health
Adhd Anorexia Depression Dyslexia Mental Health Psychiatry Schizophrenia Stress Mental health, although not being as obvious as physical health, is very important, causing great unhappiness to those affected, causing add...

Searches Linking to this Article