Track topics on Twitter Track topics that are important to you
Osteocalcin (OCN) is a bone-derived hormone involved in the regulation of glucose metabolism. In serum, OCN exists in carboxylated and uncarboxylated forms (ucOCN), and studies in rodents suggest that ucOCN is the bioactive form of this hormone. However, whether this is also the case in humans is unclear, because a reliable assay to measure ucOCN is not available. Here, we established and validated a new immunoassay (ELISA) measuring human ucOCN, and used it to determine the level of bioactive OCN in two cohorts of overweight or obese subjects, with or without type 2 diabetes (T2D). The ELISA could specifically detect ucOCN concentrations ranging from 0.037 to 1.8 ng/mL. In a first cohort of postmenopausal women overweight or obese, without diabetes (n=132), ucOCN correlated negatively with fasting glucose (r=-0.18, =0.042) and insulin resistance assessed by HOMA-IR (r=-0.18, =0.038), and positively with insulin sensitivity assessed by hyperinsulinemic-euglycemic clamp (r=0.18, =0.043) or insulin sensitivity index derived from oral glucose tolerance test (r=0.26, =0.003). In a second cohort of subjects with severe obesity (n=16), ucOCN was found to be lower in subjects with T2D compared to those without T2D (2.76 ± 0.38 vs. 4.52 ± 0.06 ng/mL, =0.009), and to negatively correlate with fasting glucose (r=-0.50, =0.046) and glycated hemoglobin (r=-0.57, =0.021). Moreover, the subjects with ucOCN levels below 3 ng/mL had reduced insulin secretion rate during a hyperglycemic clamp (=0.03). In conclusion, measurement of ucOCN with this novel and specific assay is inversely associated with insulin resistance and β-cell dysfunction in humans.
This article was published in the following journal.
Name: American journal of physiology. Endocrinology and metabolism
Immunoassay interference is most often found for Prolactin measurement. Only few data exist on immunoassay interference for other hormones.
Bone plays a role in glucose metabolism through the release of uncarboxylated osteocalcin into the systemic circulation. The identified novel roles for osteocalcin include increasing insulin secretion...
To revisit a finding, first described in 1978, which documented existence of a pituitary growth factor that escaped detection by immunoassay, but which was active in the established rat tibia GH bioas...
We examined associations between prenatal plasma oxytocin levels and depressive symptoms, state anxiety, and pregnancy anxiety in 75 women who visited the laboratory with their partners during mid-to-...
Comparison of chemiluminescence microparticle immunoassay, indirect immunofluorescence assay, linear immunoassay and multiple microbead immunoassay detecting autoantibodies in systemic lupus erythematosus.
The aim of study was to detect antinuclear antibodies (ANA) using indirect immunofluorescence assay (IIFA), linear immunoassay (LIA), chemiluminescence microparticle immunoassay (CMIA), multiple micro...
Background: Platelet-rich fibrin (PRF) and bioactive glass putty has been shown to be effective in promoting reduction in probing depth, gain in clinical attachment, and defect fill in int...
Previous reports have suggested that ALT-immunoglobulin complex was increased according to the severity of the liver disease, and high concentration of mAST and this might indicate a sever...
In the proposed study, the investigators will explore three specific aims. First, the investigators will examine cross-sectionally the association of obesity on sympathetic, parasympathet...
Spanish dry-cured ham has been shown a source of antihypertensive peptides in mice. To date, no clinical study has been performed in humans to check the effects of bioactive peptides produ...
This purpose of this study is to determine fusion rates and compare clinical outcomes of the NanoFUSE® Bioactive Matrix during post-market clinical use.
Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
The measurement around the body at the level of the ABDOMEN and just above the hip bone. The measurement is usually taken immediately after exhalation.
The measurement of magnetic fields generated by electric currents from the heart. The measurement of these fields provides information which is complementary to that provided by ELECTROCARDIOGRAPHY.
A diagnostic test is any kind of medical test performed to aid in the diagnosis or detection of disease. For example: to diagnose diseases to measure the progress or recovery from disease to confirm that a person is free from disease Clin...
Osteoporosis is a disease in which the bones become extremely porous, are subject to fracture, and heal slowly, occurring especially in women following menopause and often leading to curvature of the spine from vertebral collapse. Follow and track&n...