Measurement of bioactive osteocalcin in humans using a novel immunoassay reveals association with glucose metabolism and β-cell function.

07:00 EST 14th January 2020 | BioPortfolio

Summary of "Measurement of bioactive osteocalcin in humans using a novel immunoassay reveals association with glucose metabolism and β-cell function."

Osteocalcin (OCN) is a bone-derived hormone involved in the regulation of glucose metabolism. In serum, OCN exists in carboxylated and uncarboxylated forms (ucOCN), and studies in rodents suggest that ucOCN is the bioactive form of this hormone. However, whether this is also the case in humans is unclear, because a reliable assay to measure ucOCN is not available. Here, we established and validated a new immunoassay (ELISA) measuring human ucOCN, and used it to determine the level of bioactive OCN in two cohorts of overweight or obese subjects, with or without type 2 diabetes (T2D). The ELISA could specifically detect ucOCN concentrations ranging from 0.037 to 1.8 ng/mL. In a first cohort of postmenopausal women overweight or obese, without diabetes (n=132), ucOCN correlated negatively with fasting glucose (r=-0.18, =0.042) and insulin resistance assessed by HOMA-IR (r=-0.18, =0.038), and positively with insulin sensitivity assessed by hyperinsulinemic-euglycemic clamp (r=0.18, =0.043) or insulin sensitivity index derived from oral glucose tolerance test (r=0.26, =0.003). In a second cohort of subjects with severe obesity (n=16), ucOCN was found to be lower in subjects with T2D compared to those without T2D (2.76 ± 0.38 vs. 4.52 ± 0.06 ng/mL, =0.009), and to negatively correlate with fasting glucose (r=-0.50, =0.046) and glycated hemoglobin (r=-0.57, =0.021). Moreover, the subjects with ucOCN levels below 3 ng/mL had reduced insulin secretion rate during a hyperglycemic clamp (=0.03). In conclusion, measurement of ucOCN with this novel and specific assay is inversely associated with insulin resistance and β-cell dysfunction in humans.


Journal Details

This article was published in the following journal.

Name: American journal of physiology. Endocrinology and metabolism
ISSN: 1522-1555


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