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HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.

07:00 EST 14th January 2020 | BioPortfolio

Summary of "HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics."

Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.

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This article was published in the following journal.

Name: Journal of proteome research
ISSN: 1535-3907
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