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Zwitterionic phosphodiester-substituted neoglycoconjugates as ligands for antibodies and acute phase proteins.

07:00 EST 14th January 2020 | BioPortfolio

Summary of "Zwitterionic phosphodiester-substituted neoglycoconjugates as ligands for antibodies and acute phase proteins."

Zwitterionic modifications of glycans, such as phosphorylcholine and phosphoethanolamine are known from a range of prokaryotic and eukaryotic species and are recognised by mammalian antibodies and pentraxins; however, defined saccharide ligands modified with these zwitterionic moieties for high throughput studies are lacking. In the present study, we prepared and tested example mono- and disaccharides 6-substituted with either phosphorylcholine or phosphoethanolamine as bovine serum albumin neoglycoconjugates or printed in a microarray format for subsequent assessment of their binding to lectins, pentraxins and antibodies. C-reactive protein and anti-phosphorylcholine antibodies bound specifically to ligands with phosphorylcholine, but recognition by concanavalin A was abolished or reduced as compared to the corresponding non-zwitterionic compounds. Furthermore, in array format, the phosphorylcholine-modified ligands were recognised by IgG and IgM in sera of either non-infected or nematode-infected dogs and pigs. Thereby, these new compounds are defined ligands to allow the assessment of glycan-bound phosphorylcholine as a target of both the innate and adaptive immune systems in mammals.

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This article was published in the following journal.

Name: ACS chemical biology
ISSN: 1554-8937
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