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To probe into the effect of laser-induced fluorescence (LIF) on color and translucency of human enamel during tooth bleaching. Twenty enamel slabs were randomly assigned to be whitened by acidic 30% hydrogen peroxide (HP), neutral 30% HP, alkaline 30% HP, and distilled water, respectively, monitored by a colorimeter and Raman spectrometer simultaneously. Afterward, the parameter differences of color, translucency, Raman relative intensity, and LIF intensity between baseline and post-treatment of each bleaching cycle were calculated. The results demonstrated that the three bleaching groups resulted in increasingly prominent whitening outcome over time compared with control group, and no statistical difference was detected between them. Accordingly, the bleaching groups also engendered a same decrease tendency in fluorescence intensity (FI). However, less demineralization effect occurred on the enamel surface in neutral HP group. The correlation analysis further excluded the effect of demineralization on all the optical parameters ( > 0.05). Besides, various degrees of dependency were detected between FI and translucency parameter (TP), masking effect (ME), C*ab, W*, b*. In addition, ΔFI was associated with parameters of ΔC*ab, ΔW*, Δb*, ΔE, Δa*, and ΔME. ΔFI% was correlated with ΔC*ab, Δb*, ΔW*, and ΔE values. Thirty percent HP with different pH values could result in same variation tendency of enamel color, translucency, and FI. Plus, FI showed a strong association with enamel color and translucency alteration, which is promising for future application as a nondestructive testing method to evaluate bleaching effect and might be a novel way to investigate tooth bleaching mechanism.
This article was published in the following journal.
Name: Photobiomodulation, photomedicine, and laser surgery
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Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work.
A technique utilizing a laser coupled to a catheter which is used in the dilatation of occluded blood vessels. This includes laser thermal angioplasty where the laser energy heats up a metal tip, and direct laser angioplasty where the laser energy directly ablates the occlusion. One form of the latter approach uses an EXCIMER LASER which creates microscopically precise cuts without thermal injury. When laser angioplasty is performed in combination with balloon angioplasty it is called laser-assisted balloon angioplasty (ANGIOPLASTY, BALLOON, LASER-ASSISTED).
A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.
A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).