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This work deals with the optimization of the culture conditions of Bacillus invictae AH1 in order to increase the production level of the proteolytic activity. Response-surface methodology (RSM) was applied for the most significant fermentation parameters (concentration of wheat bran and K HPO /KH PO ) that were earlier identified by Plackett-Burman Design from seven possible factors. A central composite design was used and the quadratic regression model of producing active protease was built. A maximum protease activity was reached and validated experimentally, using a maximum wheat bran concentration (50 g/l) with increased K HPO /KH PO concentration (2.275 g/l). Protease production obtained experimentally coincident with the predicted value and the model was proven to be adequate. Interestingly, the use of RSM increased the protease production by four times (7000 U/ml) using a low-cost substrate and a culture time of 40 h, as compared to the standard culture conditions. In the second part of this study, a H O -tolerant alkaline protease produced from B. invictae AH1 with a molecular mass of about 41 kDa, noted P3, was purified by successive steps of ultrafiltration, gel filtration and ion exchange chromatography. The K and V values of the purified protease using casein, as substrate, were about 4 mg/ml and 27 μM/min, respectively. The highest enzyme activity was found at pH 9.0 and a temperature of 60 °C. In addition, the enzyme showed a quasi-total stability against H O (5% for 1 h) and against most of the tested solid and liquid detergents, suggesting its eventual use in bio-detergent formulations. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Biotechnology progress
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Inhibitors of HIV PROTEASE, an enzyme required for production of proteins needed for viral assembly.
Enzyme of the human immunodeficiency virus that is required for post-translational cleavage of gag and gag-pol precursor polyproteins into functional products needed for viral assembly. HIV protease is an aspartic protease encoded by the amino terminus of the pol gene.
Cell-surface glycoproteins and serine protease, also known as dipeptidyl-peptidase IV, that plays a role in T-lymphocyte activation. CD26 binds to ADENOSINE DEAMINASE on the T-cell surface.
A ceramidase subtype that is active at alkaline pH. It is found at high levels within the SMALL INTESTINE.
A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...