Comparison of the abilities of universal, super, and specific DNA barcodes to discriminate among the original species of Fritillariae cirrhosae bulbus and its adulterants.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Comparison of the abilities of universal, super, and specific DNA barcodes to discriminate among the original species of Fritillariae cirrhosae bulbus and its adulterants."

Fritillariae cirrhosae bulbus is a famous type of traditional Chinese medicine used for cough relief and eliminating phlegm. The medicine originates from dried bulbs of five species and one variety of Fritillaria. Recently, immature bulbs from other congeneric species, such as F. ussuriensis, have been sold as adulterants of Fritillariae cirrhosae bulbus in medicine markets owing to the high price and limited availability of the genuine medicine. However, it is difficult to accurately identify the bulbs from different original species of Fritillariae cirrhosae bulbus and its adulterants based on traditional methods, although such medicines have different prices and treatment efficacies. The present study adopted DNA barcoding to identify these different species and compared the discriminatory power of super, universal, and specific barcodes in Fritillaria. The results revealed that the super-barcode had strong discriminatory power (87.5%). Among universal barcodes, matK provided the best species resolution (87.5%), followed by ITS (62.5%), rbcL (62.5%), and trnH-psbA (25%). The combination of these four universal barcodes provided the highest discriminatory power (87.5%), which was equivalent to that of the super-barcode. Two plastid genes, ycf1 and psbM-psbD, had much better discriminatory power (both 87.5%) than did other plastid barcodes, and were suggested as potential specific barcodes for identifying Fritillaria species. Phylogenetic analyses indicated that F. cirrhosa was not a "good" species that was composed of multiple lineages, which might have affected the evaluation of the discriminatory ability. This study revealed that the complete plastid genome, as super barcode, was an efficient and reliable tool for identifying the original species of Fritillariae cirrhosae bulbus and its adulterants.


Journal Details

This article was published in the following journal.

Name: PloS one
ISSN: 1932-6203
Pages: e0229181


DeepDyve research library

PubMed Articles [10689 Associated PubMed Articles listed on BioPortfolio]

Tabu Variable Neighborhood Search for Designing DNA Barcodes.

A large number of Bio-data from multi-individual samples could be obtained by massively parallel sequencing that is a famous and efficient sequencing method. The sequences labeled with barcodes (or ta...

Surgery in Patients with Super Obesity: Medium-Term Follow-Up Outcomes at a High-Volume Center.

There is a need to determine which bariatric operations are the most effective for patients with super obesity and super-super obesity.

Bioinspired photonic barcodes for multiplexed target cycling and hybridization chain reaction.

Multiplexed detection of microRNA (miRNA) is of great value in clinical diagnosis. Here, a new type of polydopamine (PDA) encapsulated photonic crystal (PhC) barcodes are employed for target-triggerin...

Heteroepitaxial Growth of Multiblock Ln-MOF Microrods for Photonic Barcodes.

Micro/nanoscale multicolor barcodes with unique identifiability and a small footprint play significant roles in applications such as multiplexed labeling and tracking systems. In this work, we report ...

Free-Cog: Pragmatic Test Accuracy Study and Comparison with Mini-Addenbrooke's Cognitive Examination.

Canonical definitions of the dementia construct encompass deficits in both cognition and function, but most screening instruments for possible dementia address only cognitive abilities. Free-Cog is a ...

Clinical Trials [5874 Associated Clinical Trials listed on BioPortfolio]

B-slim, a Multi-source Digital Super Coach for Sustainable Weight Loss

The purpose of this study is to carry out a field trial to evaluate the effectiveness and added value of the b-SLIM digital Super Coach in a convenient sample of overweight and obese adult...

A Comparison of Cognitive Training Approaches in Psychotic Disorders

Cognitive remediation (CR) is the best treatment to improve neurocognitive abilities for individuals with psychosis, however, there is no gold standard method of cognitive remediation avai...

Clinical Comparison of Universal Adhesives in Terms of Different Application Modes on the Restoration of NCCLs

The aim of this randomized, controlled, prospective clinical trial was to evaluate the performances of two different universal adhesives and an etch&rinse adhesive in restoration of non-ca...

Comparison of Two Universal Composites in Posterior Teeth

The aim of this randomized, prospective clinical study was to compare the clinical performance of a universal light-curing, ultra-fine particle hybrid composite with a new version of this ...

Super 13 Pro & Prebiotics on the Human Intestinal Microflora

This study aim to investigate the roles of the test article in improving gastrointestinal functions and gut microbiota of humans. A total 40 healthy adults were recruited and randomly divi...

Medical and Biotech [MESH] Definitions

Comparison of various psychological, sociological, or cultural factors in order to assess the similarities or diversities occurring in two or more different cultures or societies.

Tests designed to evaluate general and specific areas of behaviors and abilities associated with memory and/or learning.

Comparison of outcomes, results, responses, etc for different techniques, therapeutic approaches or other inputs.

An agent used as a substrate in assays for cholinesterases, especially to discriminate among enzyme types.


Quick Search

DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Complementary and Alternative Medicine
Alternative medicine are whole medical systems that did not fit with conventional medicine as they have completely different philosophies and ideas on the causes of disease, methods of diagnosis and approaches to treatment. Although often overlapping, co...

Searches Linking to this Article