Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates."

Lab automation strategies have a vast potential for accelerating discovery processes. They enable higher efficiency and throughput for time-consuming screening procedures and reduce error-prone manual steps. Automating repetitive procedures can for instance support chemists in optimizing chemical reactions. Particularly, the technology of DNA-encoded libraries (DELs) may benefit from automation techniques, since translation of chemical reactions to DNA-tagged reactants often requires screening of multiple reaction parameters and evaluation of large numbers of reactants. Here, we describe a portable, automated system for reagent dispensing that was designed from open source materials. The system was validated performing amide coupling of carboxylic acids to DNA-linked amine and a micelle-mediated Povarov reaction to DNA-tagged hexahydropyrroloquinolines. The latter reaction required accurate pipetting of multiple components including different solvents and a surface-active reagent. Analysis of reactions demonstrated that the robotic system achieved high accuracy comparable to experimentation by an experienced chemist with the potential of higher throughput.


Journal Details

This article was published in the following journal.

Name: ACS combinatorial science
ISSN: 2156-8944


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