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Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates."

Lab automation strategies have a vast potential for accelerating discovery processes. They enable higher efficiency and throughput for time-consuming screening procedures and reduce error-prone manual steps. Automating repetitive procedures can for instance support chemists in optimizing chemical reactions. Particularly, the technology of DNA-encoded libraries (DELs) may benefit from automation techniques, since translation of chemical reactions to DNA-tagged reactants often requires screening of multiple reaction parameters and evaluation of large numbers of reactants. Here, we describe a portable, automated system for reagent dispensing that was designed from open source materials. The system was validated performing amide coupling of carboxylic acids to DNA-linked amine and a micelle-mediated Povarov reaction to DNA-tagged hexahydropyrroloquinolines. The latter reaction required accurate pipetting of multiple components including different solvents and a surface-active reagent. Analysis of reactions demonstrated that the robotic system achieved high accuracy comparable to experimentation by an experienced chemist with the potential of higher throughput.

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This article was published in the following journal.

Name: ACS combinatorial science
ISSN: 2156-8944
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