in vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "in vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide."

We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine-modification in translating polyproline sequence within long ORFs. We found that polyproline-motif inserted at the internal position of the protein arrests translation exclusively at low Mg2+ concentrations, and peptidylpolyproline-tRNA intrinsically destabilizes 80S ribosomes. We demonstrate that unmodified eIF5A essentially resolves such ribosome-stalling, however, the hypusine-modification drastically stimulates ability of eIF5A to rescue polyproline-mediated ribosome stalling, and is particularly important for the efficient translation of the N-terminal or long internal polyproline-motifs.


Journal Details

This article was published in the following journal.

Name: Journal of biochemistry
ISSN: 1756-2651


DeepDyve research library

PubMed Articles [23969 Associated PubMed Articles listed on BioPortfolio]

Reconstitution of yeast translation elongation and termination in vitro utilizing CrPV IRES-containing mRNA.

We developed an in vitro translation system from yeast, reconstituted with purified translation elongation and termination factors and programmed by CrPV IGR IRES-containing mRNA, which functions in t...

Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection.

Sequences within 5' UTRs dictate the site and efficiency of translation initiation. In this study, an unbiased screen designed to interrogate the 5' UTR-mediated regulation of the growth-promoting gen...

The involvement of eukaryotic initiation factor 5A in trophoblast cell function.

Abnormal growth and migration of trophoblast cells is one of the main causes of spontaneous abortion. Eukaryotic translation initiation factor 5A (eIF5A) plays an important role in trophoblast cell gr...

In vitro self-replication and multicistronic expression of large synthetic genomes.

The generation of a chemical system capable of replication and evolution is a key objective of synthetic biology. This could be achieved by in vitro reconstitution of a minimal self-sustaining central...

An in vitro single-molecule assay for eukaryotic cap-dependent translation initiation kinetics.

Eukaryotic mRNAs are predominantly translated via the cap-dependent pathway. Initiation is a rate-limiting step in cap-dependent translation and is the main target of translational control mechanisms....

Clinical Trials [9491 Associated Clinical Trials listed on BioPortfolio]

The Effect of Insoluble Yeast Beta-glucan Intake on Pre-diabetic Patients

There is a lot of evidence to show that the yeast beta-glucan has immunomodulatory, anti-inflammatory, anti-infective effects.However,few work was done on the relationship between yeast ba...

Pharmacokinetic Study of Advate Reconstituted in 2 mL Sterile Water for Injection

The purpose of this study is to determine the pharmacokinetics and safety of rAHF-PFM reconstituted in 2 mL sterile water for injection (SWFI) and compare with those of rAHF-PFM reconstitu...

Lipid-lowering Effect of Phytosterols, Red Yeast Rice and Their Combination

A large body of evidence confirm the cholesterol lowering effect of phytosterols and red yeast rice. Because their mechanisms of action mime the ones of chemical statins and cholesterol ab...

Red Rice Yeast in Low-density Lipoprotein (LDL) Lowering: an Efficacy Study

Red yeast rice is a source of active compounds in reducing LDL levels with practically no side effects. Molval Fort is a natural product available in the Lebanese market with a combination...

Safety of Red Yeast Rice for High Cholesterol in Individuals With Statin Intolerance

This study will examine the effect of red yeast rice extract compared to pravastatin on muscle related complaints in individuals with high cholesterol who have previously been unable to to...

Medical and Biotech [MESH] Definitions

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.

Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.

A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.

An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.

Quick Search

DeepDyve research library

Searches Linking to this Article