Reconstitution of yeast translation elongation and termination in vitro utilizing CrPV IRES-containing mRNA.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Reconstitution of yeast translation elongation and termination in vitro utilizing CrPV IRES-containing mRNA."

We developed an in vitro translation system from yeast, reconstituted with purified translation elongation and termination factors and programmed by CrPV IGR IRES-containing mRNA, which functions in the absence of initiation factors. The system is capable of synthesizing the active reporter protein, nanoLuciferase, with a molecular weight of 19 kDa. The protein synthesis by the system is appropriately regulated by controlling its composition, including translation factors, amino acids, and antibiotics. We found that a high eEF1A concentration relative to the ribosome concentration is critically required for efficient IRES-mediated translation initiation, to ensure its dominance over IRES-independent random internal translation initiation.


Journal Details

This article was published in the following journal.

Name: Journal of biochemistry
ISSN: 1756-2651


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Medical and Biotech [MESH] Definitions

Any codon that signals the termination of genetic translation (TRANSLATION, GENETIC). PEPTIDE TERMINATION FACTORS bind to the stop codon and trigger the hydrolysis of the aminoacyl bond connecting the completed polypeptide to the tRNA. Terminator codons do not specify amino acids.

A process of GENETIC TRANSLATION whereby the last amino acid is added to a lengthening polypeptide. This termination is signaled from the MESSENGER RNA, by one of three termination codons (CODON, TERMINATOR) that immediately follows the last amino acid-specifying CODON.

Regeneration of normal immune function after immune depleting procedures or infections (e.g., HEMATOPOIETIC STEM CELL TRANSPLANTATION). Delayed and incomplete reconstitution of the ADAPTIVE IMMUNE system in particular involving T-CELLS is associated with increase or relapse of infection.

A process of GENETIC TRANSLATION, when an amino acid is transferred from its cognate TRANSFER RNA to the lengthening chain of PEPTIDES.

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

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