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Hemerythrins enhance aerobic respiration in methylomicrobium alcaliphilum 20zr, a methane-consuming bacterium.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Hemerythrins enhance aerobic respiration in methylomicrobium alcaliphilum 20zr, a methane-consuming bacterium."

Numerous hemerythrins, di-iron proteins, have been identified in prokaryote genomes, but in most cases their function remains elusive. Bacterial hemerythrin homologs (bacteriohemerythrins, Bhrs) may contribute to various cellular functions, including oxygen sensing, metal binding and antibiotic resistance. It has been proposed that methanotrophic Bhrs support methane oxidation by supplying oxygen to a core enzyme, particulate methane monooxygenase (pMMO) (Chen et al., 2012). In this study, the consequences of the overexpression or deletion of the Bhr gene (bhr) in Methylomicrobiam alcaliphillum 20ZR were investigated. We found that the bhr-knockout (20ZRΔbhr) displays growth kinetics and methane consumption rates similar to wild type. However, the 20ZR Δbhr accumulates elevated concentrations of acetate at aerobic conditions, indicating slowed respiration. The methanotrophic strain overproducing Bhr shows increased oxygen consumption and reduced carbon-conversion efficiency, while its methane consumption rates remain unchanged. These results suggest that the methanotrophic Bhr proteins specifically contribute to oxygen-dependent respiration, while they have minimal, if any, input of oxygen for the methane oxidation machinery.

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This article was published in the following journal.

Name: FEMS microbiology letters
ISSN: 1574-6968
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