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Virulent pathotypes of seriously affect the livestock regarding the misuse of antibiotics. All 180 samples collected from cow's environment and dairy shops in Qena, Egypt were serologically and molecularly positive for coliforms. Enteropathogenic (EPEC), Shiga toxin-producing (STEC), Enteroinvasive (EIEC) and Enterotoxigenic (ETEC) pathotypes were isolated from water and milk-related samples. STEC serogroups O26, O55, O111, O113, O145 were also recovered. The non-O157 STEC serotypes were recovered from human diarrheagenic patients contacting cattle or consuming contaminated water/milk products. and genes were detected in 25.5% and 100%, respectively. Disinfectants and algal extracts, identified by GC-MS, were evaluated in vitro for antibacterial activities. TH4+® disinfectant and methanol extract of reduced at 13 log10 at 1.5% and 3 mg/ml concentrations, respectively. Ag-NPs/ showed 8-9 log10 reduction at concentration of 1.6 × 10 NPs/ml. Examined water sources, milk and milk products were potential reservoirs for virulent antibiotic-resistant which may impose animal and public health threats.
Avian pathogenic : β-lactamase inhibitors-Cefotaximase gene; : β-lactamase inhibitors-Temoneira gene;
Diffusely adherent ;
Dimethyl sulfoxide; : Intimin or attaching gene;
Egyptian Organization for Standardization and Quality Control;
Enterotoxigenic ; Ex
Extra-intestinal pathogenic ; GC-
Gas chromatography-mass spectrometry technique; : Hemolysin gene;
Shiga like producing : Shiga-toxin 1 gene; ESBLs: Extended-spectrum beta-lactamases.
This article was published in the following journal.
Name: International journal of environmental health research
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The inhabitants of rural areas or of small towns classified as rural.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work.