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A method for determining amitraz and 2,4-dimethylaniline in honey was established by using ultra-HPLC and Q Exactive after applying quick, easy, cheap, effective, rugged, and safe  extracting process. A suitable extraction method was designed to extract the amitraz and 2,4-dimethylaniline after a suitable amount of honey samples was dissolved. A Thermo Syncronis C column (100 × 2.1 mm, 1.7 μm) was used for chromatographic separation of the samples. Then the two compounds were quantitatively analyzed via a program of Q Exactive. The linearity of amitraz and 2,4-dimethylaniline was good in the concentration range of 0.5 μg/L to 100 μg/L, and the correlation coefficient R was greater than 0.99. The average recovery and relative standard deviation of each component were 81.3%-90.0% and 5.1%-7.2%. The 24- and 48-hour test results showed that the sample needed to be tested within 24 hours. The limit of detection was 0.1 μg/kg for amitraz and 2,4-dimethylaniline, whereas the limit of quantitation was 0.3 μg/kg for both. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Journal of separation science
Beehive products are considered sentinels for environmental pollutants. The presence of trace elements and pesticides in honey and beeswax may pose a health hazard to consumers. The study's aim was to...
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This study determined the glycemic index and glycemic load of six Greek honey varieties
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A sweet viscous liquid food, produced in the honey sacs of various bees from nectar collected from flowers. The nectar is ripened into honey by inversion of its sucrose sugar into fructose and glucose. It is somewhat acidic and has mild antiseptic properties, being sometimes used in the treatment of burns and lacerations.
The identification of selected parameters in newborn infants by various tests, examinations, or other procedures. Screening may be performed by clinical or laboratory measures. A screening test is designed to sort out healthy neonates (INFANT, NEWBORN) from those not well, but the screening test is not intended as a diagnostic device, rather instead as epidemiologic.
The medical use of honey bee products such as BEE VENOM; HONEY; bee pollen; PROPOLIS; and royal jelly.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Testing or screening required by federal, state, or local law or other agencies for the diagnosis of specified conditions. It is usually limited to specific populations such as categories of health care providers, members of the military, and prisoners or to specific situations such as premarital examinations or donor screening.