Topics

Screening and Quantification of TNF-α Ligand from Angelicae Pubescentis Radix by Biosensor and UPLC-MS/MS.

07:00 EST 25th February 2020 | BioPortfolio

Summary of "Screening and Quantification of TNF-α Ligand from Angelicae Pubescentis Radix by Biosensor and UPLC-MS/MS."

The aim of this study is to establish a method for rapid screening of active ingredients targeting TNF-α from Chinese herbal medicines. Take Angelicae Pubescentis Radix (APR) as an example, surface plasma resonance technique was used to establish for screening small molecule inhibitors of TNF-α from APR extract. Then UPLC-MS/MS coupled with chemometric was used for quantitative and evaluate the differences of the candidate compounds bound to TNF-α in APR from different sources. In the experiment, TNF-α protein was fixed on the CM5 chip surface of biacore T200 biosensor by amino coupling. A series of small molecular compounds in APR were screened and six phenolic acid compounds had a strong affinity for TNF-α protein and could be used as TNF-α antagonists. In summary, the targeted drug screening method for TNF-α protein based on SPR technology established in this study can be used to screen anti-TNF-α small molecule inhibitors. UPLC-MS/MS can accurately quantify 15 active ingredients, which provides reliable experimental data and new research ideas for targeted drug research on TNF-α protein.

Affiliation

Journal Details

This article was published in the following journal.

Name: Analytical biochemistry
ISSN: 1096-0309
Pages: 113643

Links

DeepDyve research library

PubMed Articles [7670 Associated PubMed Articles listed on BioPortfolio]

Deciphering the compatibility rule of Traditional Chinese Medicine prescription based on NMR metabolomics: A case study of Xiaoyaosan.

Xiaoyaosan (XYS), a represent and classic TCM prescription, which consists of Radix Bupleuri, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Poria, Herba Menthae, R...

Plasma metabolomics of depressed patients and treatment with Xiaoyaosan based on mass spectrometry technique.

Xiaoyaosan (XYS), a famous and classic traditional Chinese prescription, has been used for long time in treating depressive disorders. XYS consists of Radix Bupleuri (Bupleurum chinense DC.), Radix An...

Effect of Co-Administration of Acori Tatarinowii Rhizoma volatile oil on pharmacokinetic fate of xanthotoxol, oxypeucedanin hydrate and byakangelicin from Angelicae Dahuricae Radix in rat.

A combination of Angelicae Dahuricae Radix and Acori Tatarinowii Rhizoma has been widely used as the herb-pair in traditional Chinese medicine to treat stroke, migraine and epilepsy. However, the unde...

Radix saw: a useful tool for rhinoplasty to correct high radix.

The most difficult aspect of radix lowering is determining the maximum amount of bone that can be removed with osteotomes; here, we describe use of a radix saw, which is a new tool for determining thi...

A NMR-based drug screening strategy for discovering active substances from herbal medicines: Using Radix Polygoni Multiflori as example.

Herbal medicines have always been important sources for new drugs. And developing new drugs from traditional herbal medicine is currently still an effective way. However, screening for active substanc...

Clinical Trials [3794 Associated Clinical Trials listed on BioPortfolio]

Effectiveness of Qufeng Shengshi Fang on Treatment of Allergic Rhinitis.

This is a controlled clinical trials about traditional Chinese medicine named Qufeng Shengshi Fang,which consisted of cicada slough,bombyx batryticatus,radix angelicae,radix sileri...

OstiSense Biosensor in Bruxism Reduction - A Clinical Study

The overall objective of the clinical studies with the OstiSense biosensor tool will be to evaluate whether the use of the OstiSense biosensor tool significantly reduces the number of slee...

Point of Care Test to Diagnosed Colorectal Cancer and Polyps in Low Middle Income Countries

This is a-two phase study. Phase 1 will adapt a 3-metabolite biosensor that identifies patients with colorectal cancer (CRC) and precancerous polyps to Nigerian patients. Phase 2 will pilo...

Biosensors for Open and Closed-loop Glycemia Control in T1D Patients With Insulin Pump

In T1D, the destruction of beta-pancreatic cells causes insulin deficiency and requires insulin therapy whose control remains complex: even with recent technologies of continuous measureme...

Detection of Gastrointestinal Bleeding in Intensive Care Patients Via Biosensor Watch

The purpose of this study to improve methods of monitoring and diagnosing gastrointestinal bleeding via the E4 wristband, a biosensor watch.

Medical and Biotech [MESH] Definitions

The identification of selected parameters in newborn infants by various tests, examinations, or other procedures. Screening may be performed by clinical or laboratory measures. A screening test is designed to sort out healthy neonates (INFANT, NEWBORN) from those not well, but the screening test is not intended as a diagnostic device, rather instead as epidemiologic.

A transmembrane-protein belonging to the TNF family of intercellular signaling proteins. It is a widely expressed ligand that activates APOPTOSIS by binding to TNF-RELATED APOPTOSIS-INDUCING LIGAND RECEPTORS. The membrane-bound form of the protein can be cleaved by specific CYSTEINE ENDOPEPTIDASES to form a soluble ligand form.

Examination of urine by chemical, physical, or microscopic means. Routine urinalysis usually includes performing chemical screening tests, determining specific gravity, observing any unusual color or odor, screening for bacteriuria, and examining the sediment microscopically.

Testing or screening required by federal, state, or local law or other agencies for the diagnosis of specified conditions. It is usually limited to specific populations such as categories of health care providers, members of the military, and prisoners or to specific situations such as premarital examinations or donor screening.

A nuclear receptor protein which in humans is encoded by the RARA gene. It belongs to a family of transcription regulators of ligand-responsive regulatory proteins which include steroid hormone receptors. In addition to a C-terminal ligand-binding domain, these nuclear receptors contain a highly-conserved N-terminal zinc-finger domain that mediates binding ligand-responsive elements.

Quick Search


DeepDyve research library

Relevant Topics

Cytokine Tumour Necrosis Factor (TNF)
TNF is a compound that is classified as a cytokine which plays a central role in the cellular mechanisms of apoptosis or cell death. However, there are a number of different kinds of TNF, just under twenty, but the family of molecules have very similar a...

Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...

Blood
Blood is a specialized bodily fluid that delivers necessary substances to the body's cells (in animals) – such as nutrients and oxygen – and transports waste products away from those same cells.  In vertebrates, it is composed of blo...


Searches Linking to this Article