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Mid-infrared spectroscopy is finding an increasing number of applications; however, many of its potential uses remain unexplored. In this study, mid-infrared spectroscopy is applied to predict total antioxidant capacity and phenolic contents of powdered matrices of 14 diverse plant species. In all instances, the optimum prediction models were found using standard normal variate smoothing as a pre-processing method. The results show high correlation between the FTIR predicted and chemically determined values, namely R values of 0.962 for total phenolics, 0.829 for cupric reducing antioxidant potential (CUPRAC) and 0.911 for ferric reducing antioxidant potential (FRAP). The relative RMSE found for validation indicated that total phenolic content could be predicted with higher accuracy than CUPRAC or FRAP. This pilot study highlights the promise of this technology for plant breeders and a range of industries where rapid screening of many samples for antioxidant and/or phenolic content is envisaged.
This article was published in the following journal.
Name: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)
A noninvasive technique that uses the differential absorption properties of hemoglobin and myoglobin to evaluate tissue oxygenation and indirectly can measure regional hemodynamics and blood flow. Near-infrared light (NIR) can propagate through tissues and at particular wavelengths is differentially absorbed by oxygenated vs. deoxygenated forms of hemoglobin and myoglobin. Illumination of intact tissue with NIR allows qualitative assessment of changes in the tissue concentration of these molecules. The analysis is also used to determine body composition.
Measurement of the regional temperature of the body or an organ by infrared sensing devices, based on self-emanating infrared radiation.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.