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Sensitivity enhancement in 2D Double Cross Polarization experiments under fast MAS by recycling unused protons.

07:00 EST 13th February 2020 | BioPortfolio

Summary of "Sensitivity enhancement in 2D Double Cross Polarization experiments under fast MAS by recycling unused protons."

We demonstrate sensitivity enhancement via recycling of proton magnetization in 2D Double Cross Polarization (Double CP) experiments performed on fully protonated and uniformly labeled (C, N) samples at a magic angle spinning rate of 60 kHz. Unused proton magnetization is preserved during t evolution either by locking it with CW irradiation or by employing rotor-synchronized pi pulses. A flip-back pulse together with a modified second CP block preserves unused proton magnetization resulting in enhanced sensitivity. We have achieved sensitivity enhancements of 15-20% and 25-28% in H-C and H-N 2D Double CP experiments respectively. At shorter recycle delays (∼0.25T), relative sensitivity enhancements of 40-45% and 55% were obtained in H-C and H-N 2D Double CP experiments respectively. An analysis of the sensitivity enhancements and theoretical estimation of lineshapes in indirect dimension in the presence of proton recycling is provided.

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This article was published in the following journal.

Name: Solid state nuclear magnetic resonance
ISSN: 1527-3326
Pages: 101652

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