Topics

An efficient method for generating murine hypothalamic neurospheres for the study of regional neural progenitor biology.

08:00 EDT 10th March 2020 | BioPortfolio

Summary of "An efficient method for generating murine hypothalamic neurospheres for the study of regional neural progenitor biology."

The hypothalamus is a key homeostatic brain region and the primary effector of neuroendocrine signaling. Recent studies show that early embryonic developmental disruption of this region can lead to neuroendocrine conditions later in life, suggesting that hypothalamic progenitors might be sensitive to exogenous challenges. To study the behavior of hypothalamic neural progenitors, we developed a novel dissection methodology to isolate murine hypothalamic neural stem and progenitor cells at the early timepoint of embryonic day (E) 12.5, which coincides with peak hypothalamic neurogenesis. Additionally, we established and optimized a culturing protocol to maintain multipotent hypothalamic neurospheres that are capable of sustained proliferation or differentiation into neurons, oligodendrocytes, and astrocytes. We characterized media requirements, appropriate cell seeding density, and the role of growth factors and sonic hedgehog (Shh) supplementation. Finally, we validated the use of fluorescence activated cell sorting of either Sox2GFPKI or Nkx2.1GFPKI transgenic mice as an alternate cellular isolation approach to enable enriched selection of hypothalamic progenitors for growth into neurospheres. Combined, we present a new technique that yields reliable culturing of hypothalamic neural stem and progenitor cells that can be used to study hypothalamic development in a controlled environment.

Affiliation

Journal Details

This article was published in the following journal.

Name: Endocrinology
ISSN: 1945-7170
Pages:

Links

DeepDyve research library

PubMed Articles [26734 Associated PubMed Articles listed on BioPortfolio]

A rapid and efficient method for the collection of highly developmental murine immature oocytes using cilostazol, a phosphodiesterase 3A inhibitor.

The present study aims to define maturation, yield, health, and ease of collection of murine immature oocytes recovered using the conventional method or from mice treated with cilostazol.

PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations.

In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the...

The economic value of stochastic climate information for agricultural adaptation in a semi-arid region in Austria.

Identifying efficient adaptation measures in land and water use requires integrated approaches and a spatially and temporally explicit representation of water demand and supply. Stochastic climate inf...

Pareto law-based regional inequality analysis of PM2.5 air pollution and economic development in China.

Regional inequality has caused large social and economic problems in China. Numerous researchers have sought to understand the status of economic inequality in the past decades. However, studies are l...

Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination.

Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-...

Clinical Trials [5068 Associated Clinical Trials listed on BioPortfolio]

Study of Hypothalamic Metabolism in Spontaneous Cluster Headache Attacks

Cluster headache is characterized by unilateral excruciating head pain and autonomic dysfunction. Hypothalamic overactivity was observed in nitrate-induced cluster-headache and in only one...

Regional Block of the Nose for Hypotensive Anaesthesia in Septo-rhinoplasty

Septorhinoplasty is a cosmetic surgery that many people are choosing to undergo. It can be done under general anesthesia, twilight anesthesia or regional anesthesia. Controlled hypotensive...

Regional Anesthesia to Relief Zoster Pain

Pain in Herpes is a serious issue for patients. at the acute phase it is a moderate to severe pain . 30% patients will develop a post herpetic neuralgia this percentage up to 70 % after 60...

Application of Indicator Based-electrical Impedance Tomography Method Assess Lung Regional Perfusion in ICU Patients

This study is an observational study. Investigate the relationship of oxgenation and lung regional ventilation and perfusion assessed by the indicator based-EIT method in the critically il...

Regional Anesthesia for Modified Radical Mastectomy With Axillary Lymph Node Dissection

local and regional anesthesia have been introduced with the goal of reducing the side effects associated with general anesthesia and IV opioid analgesia.our hypothesis is that ultrasound-g...

Medical and Biotech [MESH] Definitions

First-released version of study results in a series of data collection efforts used for the purpose of generating further interest in and or funding of a research study.

A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of transforming lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; MOLONEY MURINE LEUKEMIA VIRUS; or RAUSCHER VIRUS.

A replication-defective murine sarcoma virus (SARCOMA VIRUSES, MURINE) capable of transforming mouse lymphoid cells and producing erythroid leukemia after superinfection with murine leukemia viruses (LEUKEMIA VIRUS, MURINE). It has also been found to transform cultured human fibroblasts, rat liver epithelial cells, and rat adrenocortical cells.

A group of replication-defective viruses, in the genus GAMMARETROVIRUS, which are capable of transforming cells, but which replicate and produce tumors only in the presence of Murine leukemia viruses (LEUKEMIA VIRUS, MURINE).

The anatomical study of specific regions or parts of organisms, emphasizing the relationship between the various structures (e.g. muscles, nerves, skeletal, cardiovascular, etc.).

Quick Search


DeepDyve research library

Relevant Topics

Endocrinology
Diabetes Diabetes Endocrine Disorders Obesity Oxycontin Renal Disease Thyroid Disorders Endocrinology is the study of the endocrine glands and the hormones that they secrete (Oxford Medical Dictionary). There are several g...

Endocrine Disorders
Endocrine disorders are grouped into two categories: hormone imbalance - when a gland produces too much or too little of an endocrine hormone development of lesions (such as nodules or tumors) in the endocrine system, which may or may not affect...

Stem Cells
Track and monitor developments in stem cell research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on stem cells and follow companies active in the stem cell industry.  BioPort...


Searches Linking to this Article