Topics

Comparison of gene expression of pyruvate kinase and tryparedoxin peroxidase in metacyclic promastigote forms of Leishmania (L.) tropica and L. major by real-time PCR

07:00 EST 1st January 2020 | BioPortfolio

Summary of "Comparison of gene expression of pyruvate kinase and tryparedoxin peroxidase in metacyclic promastigote forms of Leishmania (L.) tropica and L. major by real-time PCR"

Two predominant forms of cutaneous leishmaniosis are anthroponotic CL (ACL) and zoonotic CL (ZCL) caused by Leishmania (L.) tropica and L. major in Iran and many countries, respectively. Since differential gene expression play an important role in outcome of the infection, we compared relative gene expression value of pyruvate kinase (PyrK) and tryparedoxin peroxidase (TryP) in metacyclic forms of Iranian isolates of L. major and L. tropica. Clinical isolates of CL patients were sampled in endemic foci of Iran and identified by PCR-RFLP. Then, we employed real-time PCR to evaluation of the expression level of PyrK and Tryp genes in L. major and L. tropica. By this comparison, up-regulation of PyrK and Tryp genes was observed in metacyclic stage. Moreover, the average mRNA expression of PyrK and Tryp genes in L. major was 1.69 and 3.72 folds of its expression in L. tropica isolates. The results of this study could open the new window for further investigations of the correspondence between parasite gene expression level and disease pathology. Species-specific parasite factors contributing to virulence and pathogenicity in the host may be mostly due to the some of the differential regulation of conserved genes between species.

Affiliation

Journal Details

This article was published in the following journal.

Name: Annals of parasitology
ISSN: 2299-0631
Pages: 13-18

Links

DeepDyve research library

PubMed Articles [19510 Associated PubMed Articles listed on BioPortfolio]

Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells.

MicroRNAs (miRNAs) can act as negative regulators of gene expression, and play a crucial role in cancer progression. The aim of this study was to investigate the role of miR-1294/pyruvate kinase M2 (P...

Safety and Efficacy of Mitapivat in Pyruvate Kinase Deficiency.

Pyruvate kinase deficiency is caused by mutations in and leads to congenital hemolytic anemia. Mitapivat is an oral, small-molecule allosteric activator of pyruvate kinase in red cells.

The synthetic oleanane triterpenoid CDDO-Me binds and inhibits pyruvate kinase M2.

The M2 isoform of the glycolytic enzyme pyruvate kinase (PKM2) is one of the key components in the Warburg effect, and an important regulator of cancer cell metabolism. Elevated PKM2 expression is a h...

The phosphoenolpyruvate-pyruvate-oxaloacetate node genes and enzymes in Streptomyces coelicolor M-145.

The phosphoenolpyruvate-pyruvate-oxaloacetate node is a major branch within the central carbon metabolism and acts as a connection point between glycolysis, gluconeogenesis, and the TCA cycle. Phospho...

Gene Regulatory Effect of Pyruvate Kinase M2 is Involved in Renal Inflammation in Type 2 Diabetic Nephropathy.

The inflammation of glomerular endothelial cells induces and promotes the activation of macrophages and contributes to the development of diabetic nephropathy. Thus, this study aimed to investigate th...

Clinical Trials [7842 Associated Clinical Trials listed on BioPortfolio]

Pyruvate Kinase Deficiency Epidemiological Study

Pyruvate kinase deficiency (PKD) is the most common red cell glycolytic enzyme defect causing hereditary non-spherocytic hemolytic anemia, caused by mutations in the PKLR gene. The main go...

Gene Therapy for Pyruvate Kinase Deficiency (PKD)

This is an open-label Phase I trial to evaluate the safety of a hematopoietic cell-based gene therapy for patients with Pyruvate Kinase Deficiency (PKD).

A Study of AG-348 in Adult Patients With Pyruvate Kinase Deficiency

Study AG348-C-003 is a multicenter study designed to evaluate the safety and efficacy of different dose levels of AG-348 in patients with PK deficiency.

Genotype -Phenotype Correlation of PKLR Variants With Pyruvate Kinase, 2,3-Diphosphglycerate and Adenosine Triphosphate Activities in Red Blood Cells of People With Sickle Cell Disease

Background: Some people with the same disorder on a genetic level have more complications than others. Researchers want to look for a link between the gene PKLR and sickle cell disease (S...

Pyruvate Kinase Deficiency Global Longitudinal Registry

This study is an observational (ie, non-interventional), longitudinal, multicenter, global registry for patients with pyruvate kinase (PK) deficiency, a rare non-spherocytic hemolytic anem...

Medical and Biotech [MESH] Definitions

ATP:pyruvate 2-O-phosphotransferase. A phosphotransferase that catalyzes reversibly the phosphorylation of pyruvate to phosphoenolpyruvate in the presence of ATP. It has four isozymes (L, R, M1, and M2). Deficiency of the enzyme results in hemolytic anemia. EC 2.7.1.40.

A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression.

The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.

A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.

An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.

Quick Search
DeepDyve research library

Relevant Topics

Gene Expression
The process of gene expression is used by eukaryotes, prokaryotes, and viruses to generate the macromolecular machinery for life. Steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-tran...

Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...

Infectious-diseases
Antiretroviral Therapy Clostridium Difficile Ebola HIV & AIDS Infectious Diseases Influenza Malaria Measles Sepsis Swine Flu Tropical Medicine Tuberculosis Infectious diseases are caused by pathogenic...


Searches Linking to this Article