Detection of a Novel Mutation in the rpoB gene in a Multidrug Resistant Mycobacterium tuberculosis Isolate using Whole Genome Next Generation Sequencing.

08:00 EDT 18th March 2020 | BioPortfolio

Summary of "Detection of a Novel Mutation in the rpoB gene in a Multidrug Resistant Mycobacterium tuberculosis Isolate using Whole Genome Next Generation Sequencing."

Mycobacterium tuberculosis (Mtb) drug resistance is a global concern. Moreover, multiple drug resistant (MDR), extensively drug resistant (XDR), and totally drug resistant (TDR) Mtb cases are on the rise in developing countries like India. Most of these cases are identified only 3-6 months after initiation of treatment owing to incomplete/failed clinical response and incomplete information from phenotypic drug resistance assays and/or targeted Mtb mutation analysis. Here, we report the development of an in-house whole genome sequencing (WGS) assay and bioinformatics pipeline that helped resolve the phenotype-genotype discrepancy in a clinical isolate.


Journal Details

This article was published in the following journal.

Name: Journal of global antimicrobial resistance
ISSN: 2213-7173


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Medical and Biotech [MESH] Definitions

Tuberculosis resistant to chemotherapy with two or more ANTITUBERCULAR AGENTS, including at least ISONIAZID and RIFAMPICIN. The problem of resistance is particularly troublesome in tuberculous OPPORTUNISTIC INFECTIONS associated with HIV INFECTIONS. It requires the use of second line drugs which are more toxic than the first line regimens. TB with isolates that have developed further resistance to at least three of the six classes of second line drugs is defined as EXTENSIVELY DRUG-RESISTANT TUBERCULOSIS.

Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).

A mutation that results in an increase in a gene's activity or in acquiring a new molecular function or a new pattern of gene expression.

The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.

A mutation that causes a decrease in or elimination of a gene product's activity.

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