Topics

Unique observation method of temperature dependence of diatom floating by direct microscope.

08:00 EDT 18th March 2020 | BioPortfolio

Summary of "Unique observation method of temperature dependence of diatom floating by direct microscope."

Diatoms are one of the earth's major oxygen producers. For that reason, studying the floating phenomena of living diatom cells in water is an important research subject. Efficiency of photosynthesis of diatom cells may be heavily affected by their floating behavior. In our previous research, we devised a 'tumbled' microscope, a device created by tilting an inverted microscope (CKX53, OLYMPUS) by 90 degrees, due to which allowed observation with a sample stage perpendicular to the ground. When we observed a Petri dish filled with diatom cell suspension, the floating behavior of diatom cells were well visualized. Cyclotella meneghiniana was isolated and subcultured in bold modified basal freshwater nutrient solution liquid medium (B5282-500ML) at 18 °C. Before the microscopic observation, cell suspension was cultured for two weeks after the final subculture. Observation was performed at room temperature, 30 °C, and 40 °C with a temperature sensor in the center of the chamber (inside). Observations were started as soon as the sample was installed. In a typical image obtained using the tumbled microscope, the diatom cells were found to move from the top to the bottom. In order to analyze floating velocity and trajectory, observation was continued for 35 min at room temperature, 30 °C, and 40 °C. Tracking analysis was carried out using the two-dimensional motion image measurement software Move-tr/2D. The average speed of 100 cells was 7.0 ± 4.3 μm/s at room temperature, 85.6 ± 31.9 μm/s at 30 °C and 470.1 ± 279.8 μm/s at 40 °C. In this study, we devised the unique observation to visualize the temperature dependence of diatom cells.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of microbiological methods
ISSN: 1872-8359
Pages: 105901

Links

DeepDyve research library

PubMed Articles [21534 Associated PubMed Articles listed on BioPortfolio]

Improved lipid production in oleaginous brackish diatom MACC8 using two-stage cultivation approach.

A two-stage cultivation method involving the initial growth in optimized conditions for biomass production followed by those for lipid production in oleaginous brackish diatom MACC8 resulted in a pro...

Floating macro- and microplastics around the Southern Ocean: Results from the Antarctic Circumnavigation Expedition.

While macroplastics have been washing up on Southern Ocean islands for decades and microplastics have been found in seabirds from the region since 1960, there are still relatively few quantitative dat...

Direct observation of paramagnetic spin fluctuations in LaFe13-xSix.

Spin fluctuations are a crucial driving force for magnetic phase transitions, but their presence usually is indirectly deduced from macroscopic variables like volume, magnetization or electrical resis...

Diatom Frustule Silica Exhibits Superhydrophilicity and Superhemophilicity.

Special surface wettability attracts significant attention. In this study, dramatic differences in wettability are demonstrated for microparticles with the same chemical composition, SiO2. One is natu...

Dose Response and Temperature Dependence of the Mortality of Spider Mite and Predatory Mite Eggs Caused by Daily Nighttime Ultraviolet-B Irradiation.

The two-spotted spider mite, Tetranychus urticae, is an economically important agricultural pest. A novel physical control method involving daily nighttime UV-B irradiation was recently developed for ...

Clinical Trials [8043 Associated Clinical Trials listed on BioPortfolio]

Floating for Chronic Pain

In recent years there has been a novel approach for the treatment of chronic pain: Floatation REST (restricted environmental stimulation therapy). Floating is a therapeutic approach, in wh...

Float First: An Index of Floating: Factors Affecting Floating in Different Populations

The study involves measurements of body composition using a DEXA scanner, lung function, buoyancy and floating technique. An understanding of the effects of physiological and anthropometri...

Comparison of Pain Level During Intrauterine Device (IUD) Insertion Between Conventional and Direct Method

The so-called "direct" method of intrauterine device (IUD) and intraintuerine system (IUS) insertion is increasingly used. A study has shown that it is technically simpler and assumes less...

Prospective Randomized Study to Evaluate the Effect of Embryonic Observation on the Gestation (PROdE)

In a conventional in vitro fertilization (IVF) cycle, daily microscopic observation of embryos outside the incubator to assess their morphology and establish a selection process is perform...

Examining the Effects of Reduced Environmental Stimulation on Anxiety

The studies proposed in this protocol aim to explore the anxiolytic properties of floating as it relates to the central and autonomic nervous system.

Medical and Biotech [MESH] Definitions

A treatment method in which patients are under direct observation when they take their medication or receive their treatment. This method is designed to reduce the risk of treatment interruption and to ensure patient compliance.

The act of regarding attentively and studying facts and occurrences, gathering data through analyzing, measuring, and drawing conclusions, with the purpose of applying the observed information to theoretical assumptions. Observation as a scientific method in the acquisition of knowledge began in classical antiquity; in modern science and medicine its greatest application is facilitated by modern technology. Observation is one of the components of the research process.

Method to determine the occurrence of OVULATION by direct or indirect means. Indirect methods examine the effects of PROGESTERONE on cervical mucus (CERVIX MUCUS), or basal body temperature. Direct ovulation detection, generally used in fertility treatment, involves analyses of circulating hormones in blood and ULTRASONOGRAPHY.

The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.

Quick Search


DeepDyve research library

Searches Linking to this Article