The origin of genomic N-methyl-deoxyadenosine in mammalian cells.

08:00 EDT 23rd March 2020 | BioPortfolio

Summary of "The origin of genomic N-methyl-deoxyadenosine in mammalian cells."

The proposal that N-methyl-deoxyadenosine (mdA) acts as an epigenetic mark in mammals remains controversial. Using isotopic labeling coupled to ultrasensitive mass spectrometry, we confirm the presence of low-level mdA in mammalian DNA. However, the bulk of genomic mdA originates from ribo-N-methyladenosine, which is processed via the nucleotide-salvage pathway and misincorporated by DNA polymerases. Our results argue against mdA acting as a heritable, epigenetic DNA mark in mammalian cells.


Journal Details

This article was published in the following journal.

Name: Nature chemical biology
ISSN: 1552-4469


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Medical and Biotech [MESH] Definitions

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