Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides."

Advances in synthetic biology and metabolic engineering have proven the potential of introducing metabolic by-passes within cell factories. These pathways can provide a more efficient alternative to endogenous counterparts due to their insensitivity to host's regulatory mechanisms. In this work, we replaced the endogenous essential 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the industrially relevant bacterium Rhodobacter sphaeroides by an orthogonal metabolic route. The native 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway was successfully replaced by a heterologous mevalonate (MVA) pathway from a related bacterium. The functional replacement was confirmed by analysis of the reporter molecule amorpha-4,11-diene after cultivation with [4- C]glucose. The engineered R. sphaeroides strain relying exclusively on the MVA pathway was completely functional in conditions for sesquiterpene production and, upon increased expression of the MVA enzymes, it reached even higher sesquiterpene yields than the control strain coexpressing both MEP and MVA modules. This work represents an example where substitution of an essential biochemical pathway by an alternative, heterologous pathway leads to enhanced biosynthetic performance.


Journal Details

This article was published in the following journal.

Name: Microbial biotechnology
ISSN: 1751-7915


DeepDyve research library

PubMed Articles [11939 Associated PubMed Articles listed on BioPortfolio]

Removal of mercury(II), lead(II) and cadmium(II) from aqueous solutions using Rhodobacter sphaeroides SC01.

Microorganisms and microbial products can be highly efficient in uptaking soluble and particulate forms of heavy metals, particularly from solutions. In this study, the removal efficiency, oxidative d...

Engineering the transcriptional activator NifA for the construction of Rhodobacter sphaeroides strains that produce hydrogen gas constitutively.

Purple non-sulfur photosynthetic bacteria such as Rhodobacter sphaeroides and Rhodopseudomonas palustris produce hydrogen gas (H) via proton reduction, which is catalyzed by nitrogenase. Although the ...

Influence of Hydrogen Bonds on the Electron-Phonon Coupling Strength/Marker Mode Structure and Charge Separation Rates in Reaction Centers from Rhodobacter sphaeroides.

Low-temperature persistent and transient hole-burning (HB) spectra are presented for the triple hydrogen-bonded L131LH+M160LH+M197FH mutant of Rhodobacter sphaeroides. These spectra expose the heterog...

Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry.

Data from earlier studies showed that minor structural changes at the surface of cytochrome c oxidase, near one of the proton-input pathways (the D pathway), result in dramatically decreased activity ...

Cognition, Frailty, and Functional Outcomes of Transcatheter Aortic Valve Replacement.

Cognitive impairment and frailty are highly prevalent in older adults undergoing transcatheter aortic valve replacement. This study aimed to investigate the relationship of cognitive impairment and fr...

Clinical Trials [6226 Associated Clinical Trials listed on BioPortfolio]

Unrestricted Rehabilitation Pathway Following Total Hip Replacement

The purpose of ReHip2 is to explore the impact on function and quality of life in patients following Total Hip Replacement (THR). A randomised controlled trial will compare two different r...

A Multicenter Study to Evaluate the ConforMIS iTotal® (CR) Knee Replacement System Versus Off-the-Shelf Replacement

Patients will be randomized to either ConforMIS iTotal Knee Replacement or Off the Shelf knee replacement. The patient will conduct functional testing and answer questionnaires. Th...

Retrospective Evaluation of Clinical and Functional Results and Survivorship of Total Knee Replacement and Total Hip Replacement, Both Isolated or Combined.

Retrospective evaluation of clinical and functional results and survivorship of total knee replacement and total hip replacement, both isolated or combined.

Retrospective Clinical and Functional Evaluation of Patients Undergoing Knee Replacement With Small Implants

Retrospective evaluation of: Functional results Clinical results Survivorship of knee replacement performed with small implants, isolated or combined

Brain Excitability and Connectivity in Sensory-motor Pathways in ALS

The main objective is to determine the origin of somatosensory alteration in patients with ALS and to evaluate its impact on brain activity by coupling different imaging modalities and ind...

Medical and Biotech [MESH] Definitions

Spherical phototrophic bacteria found in mud and stagnant water exposed to light.

Therapeutic replacement or supplementation of defective or missing enzymes to alleviate the effects of the enzyme deficiency (e.g., GLUCOSYLCERAMIDASE replacement for GAUCHER DISEASE).

Partial or total replacement of a joint.

Replacement of the hip joint.

A complete denture constructed for replacement of natural teeth immediately after their removal. It does not fit the mouth perfectly and is intended only for functional and cosmetic purposes during the healing process after total extraction. It is to be replaced by the fitted permanent denture.

Quick Search

DeepDyve research library

Searches Linking to this Article