Effects of partial hydrolysis on the structural, functional and antioxidant properties of oat protein isolate.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Effects of partial hydrolysis on the structural, functional and antioxidant properties of oat protein isolate."

The degree of hydrolysis (DH) plays important roles in the characteristics of food proteins. Herein, in order to explore the effects of partial hydrolysis on the structural, functional and antioxidant characteristics of hydrolysates, oat protein isolate was partially hydrolyzed with alcalase at different DHs (2%, 4%, 6%, 8%, 12%, and 16%). Our results showed that hydrolysis could induce significant structural changes in oat protein, mainly reflecting at the amino acid pattern, molecular weight profile and protein conformation. Alcalase hydrolysis also resulted in hydrolysates with the emulsifying activity index of at least 19.83 m2 g-1, and the highest emulsion stability was observed in the hydrolysate with a DH of 6%, possibly due to its suitable molecular weight, exposed hydrophobic amino acid residues and high surface net charge. Besides, all hydrolysates exerted excellent DPPH radical scavenging activity with an IC50 value ranging from 19.23 to 30.32 μg mL-1, which was closely correlated with DH. The oat protein isolate with moderate alcalase hydrolysis (DH 6%) exhibited the strongest metal ion-chelating activity and possessed the maximum amount of hydrophilic amino acids. More importantly, the oat protein hydrolysate with a DH of 6% not only prolonged the induction period of sunflower oil, but also improved the stability of the sunflower oil-in-water emulsion, as evidenced by the reduced TBARS production and the homogeneous droplet size. Therefore, partial hydrolysis can be advantageous for improving the functional and antioxidant characteristics of oat protein isolate, particularly the hydrolysate with a DH of 6%.


Journal Details

This article was published in the following journal.

Name: Food & function
ISSN: 2042-650X


DeepDyve research library

PubMed Articles [38557 Associated PubMed Articles listed on BioPortfolio]

Antioxidant and cytoprotective effect of peptides produced by hydrolysis of whey protein concentrate with trypsin.

Whey protein is one of the most relevant co-products manufactured by the dairy industry and it is a powerful environmental pollutant. Therefore, the enzymatic hydrolysis of whey protein concentrate (W...

Could choline chloride-citric acid monohydrate molar ratio in deep eutectic solvent affect structural, functional and antioxidant properties of pectin?

The deep eutectic solvents (DESs), which were made from different molar ratios (3:1, 2:1, 1:1, 1:2, 1:3) of choline chloride and citric acid monohydrate, were used as media for the pectic polysacchari...

Prevention of protein oxidation and enhancement of gel properties of silver carp (Hypophthalmichthys molitrix) surimi by addition of protein hydrolysates derived from surimi processing by-products.

Surimi processing industry generates huge quantities of underutilized surimi processing by-products (SPB). This study aimed to investigate the potential antioxidant-cryoprotective and gelation-enhanci...

Impact of combined ultrasound-microwave treatment on structural and functional properties of golden threadfin bream (Nemipterus virgatus) myofibrillar proteins and hydrolysates.

The effects of microwave, ultrasound and combined ultrasound-microwave (UM) treatment with different intensities on structural and hydrolysis properties of myofibrillar protein (MP) were investigated....

Effects of Maillard reaction on physicochemical and functional properties of walnut protein isolate.

In this study, the Maillard reaction (MR) of glucose was applied to improve the physicochemical and functional properties of walnut protein isolate (WNPI). The MR products (MRPs) were prepared with gl...

Clinical Trials [11806 Associated Clinical Trials listed on BioPortfolio]

Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed pro...

Glutamine and Intestinal Protein Metabolism

Gut barrier plays a major role in defence of the organism. During catabolic states, like major surgery or inflammation, gut barrier could be altered. It has been reported that preoperative...

Barley Protein and CVD

The Question posed is: Does an barley protein concentrate have health benefits similar to those demonstrated for soy protein foods which would justify the use of the non-fiber components o...

Assessment of Coronary Plaque Composition Using Optical Coherence Tomography

The investigators hypothesis is that local activation of the endogenous Lp-PLA2 plays an integral role in early atherosclerosis, and contributes to the mechanism of coronary endothelial dy...

Matrix Aging and Aneurysm

During their biological life, proteins undergo molecular aging due to many non-enzymatic post-translational modifications that alter their structural and functional properties. These react...

Medical and Biotech [MESH] Definitions

The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.

A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.

A partial denture constructed before the teeth it replaces are removed. It is then inserted immediately after the removal of the natural teeth for functional and cosmetic reasons during the healing process. It is to be replaced later by the fitted partial denture.

Partial proteins formed by partial hydrolysis of complete proteins.

Hematologic diseases caused by structural or functional defects of BLOOD PROTEINS.

Quick Search

DeepDyve research library

Relevant Topics

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...

Searches Linking to this Article