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Comprehensive in vitro metabolism study of bisphenol A using liquid chromatography-high resolution tandem mass spectrometry.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Comprehensive in vitro metabolism study of bisphenol A using liquid chromatography-high resolution tandem mass spectrometry."

Bisphenol A (BPA) metabolism has been investigated using several in vitro models, including human and rat liver microsomes and subcellular (S9) fractions, as well as human-recombinant Cytochrome P450 3A4 (CYP3A4) expressed in Supersomes, for a comprehensive look at all possible metabolic pathways. By an untargeted approach using liquid chromatography coupled to a high-resolution quadrupole-time of flight mass spectrometer, we were able to detect a large number of known Phase I and Phase II metabolites of BPA, as well as several previously uncharacterized ones. A detailed fragmentation study of BPA and its detected metabolites was crucial to confirm structures. Isotope-labeled BPA analogs were highly useful for the structural elucidation of many metabolites. These results contribute to a better understanding of BPA metabolism, including pathways that may introduce additional toxicity, as well as help with the assessment of BPA exposure in different biological matrices.

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This article was published in the following journal.

Name: Chemical research in toxicology
ISSN: 1520-5010
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Medical and Biotech [MESH] Definitions

Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.

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Chromatographic techniques in which the mobile phase is a liquid.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

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