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Pesticides, widely used in modern agriculture, could potentially cause environmental pollution and affect human lives. Hence, the development of a highly sensitive sensing element to detect pesticide residues is crucial for food safety and ecosystem protection. Optical methods based on fluorescence properties provide an ideal approach for screening and quantification of these compounds in different medias including water, plant, and nutritional products. The development of fluorescence emitting carbon dot-based sensors for monitoring pesticides has attracted great attention in recent years. In comparison to other fluorophores, carbon dots have more promising optical features, higher quantum yields and better biocompatibility. This article aims to present a novel fluorescent sensing method of diazinon, glyphosate, and amicarbazone using plant-based carbon dots. A comprehensive characterization of carbon dots obtained from cauliflower was performed by methods including UV-visible, FTIR spectroscopy, fluorometry, AFM, DLS, and zeta sizer. Following this step, carbon dots were used to detect pesticides. The fluorescence quenching property of carbon dots has been utilized to identify detection limit of 0.25, 0.5, and 2 ng ml-1 for diazinon, amicarbazone, and glyphosate, respectively. Also, real sample study revealed that the detection of pesticides accompanied by our developed nano-sensor is repeatable and accurate. According to carbon dots specificity determination, the prepared nano sensor does not have the potential to identify "bromacil" and "dialen super" pesticides but the other three mentioned pesticides are detectable. The results confirm that synthesized green carbon dots are well qualified for application in food safety and environmental monitoring.
This article was published in the following journal.
Name: PloS one
Quantitative real-time PCR (qPCR) is a widely adopted technique used for scientific, clinical, diagnostic, or quality control purposes. One of the main applications of qPCR is gene expression analysis...
An ultrasensitive and rapid detection of trace antibiotics is imperative for food safety and public health. Herein, we present a ratiometric fluorescent sensing strategy based on an aptamer labeled wi...
Vaspin is a protein present in human serum that can cause type-2 diabetes, obesity, and other cardiovascular diseases. We report fluorescent upconverting nanoparticles (UCNPs)-based lateral flow biose...
The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investi...
The ultrasensitive detection of fibroin in unearthed silk relics has great significance for investigating the origin and transmission of silk. In this study, an anti-fibroin monoclonal antibody was su...
Fluorescence-navigated thoracoscopic imaging with indocyanine green (ICG) is a novel technique for detection of small pulmonary nodules other than traditional radiography or intraoperative...
The goal of this clinical research study is to find lymph nodes before surgery using a dye called indocyanine green (IC-Green). Objectives: 1. To determine the feasibility of usi...
This study aims to develop a standardized universal imaging protocol for ICG-guided fluorescent total thyroidectomy, including quantitative evaluations of the fluorescent signal. Therefore...
A current gap in the use indocyanine green fluorescence to isolate the biliary system is the dosing amount necessary to fluoresce the biliary system without "over" fluorescing the liver an...
Standard white light endoscopy involves the passage of a thin, flexible camera into the colon from the anus. Although standard white light endoscopy can detect most polyps and precancerou...
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent.
Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...
Food safety is a scientific discipline describing handling, preparation, and storage of food in ways that prevent foodborne illness. This includes a number of routines that should be followed to avoid potentially severe health hazards. The five key pri...