Real-time detection of foodborne bacterial viability using a colorimetric bienzyme system in food and drinking water.

08:00 EDT 10th March 2020 | BioPortfolio

Summary of "Real-time detection of foodborne bacterial viability using a colorimetric bienzyme system in food and drinking water."

Foodborne bacterial infection poses a serious threat to human health. As most diseases are caused by living bacteria, real-time assessment of bacterial viability is vitally important to the public health sector. Herein, we developed a simple and novel colorimetric assay based on the Glucose oxidase (GOD)/Horseradish peroxidase (HRP) bienzyme system for real-time monitoring of bacterial viability in food and drinking water. This bienzyme system is free of any chemical synthesis and only requires 3 sample handling steps. The color response is easily observable with the naked eye or recordable with a smartphone for precise determination of bacterial viability. The proposed strategy was validated with various bacteria both Gram-positive and Gram-negative, indicating its capability for broad-spectrum bacteria viability detection. Therefore, the proposed strategy shows promise for rapid and reliable quality control in food and drinking water.


Journal Details

This article was published in the following journal.

Name: Food chemistry
ISSN: 1873-7072
Pages: 126581


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