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Real-time detection of foodborne bacterial viability using a colorimetric bienzyme system in food and drinking water.

08:00 EDT 10th March 2020 | BioPortfolio

Summary of "Real-time detection of foodborne bacterial viability using a colorimetric bienzyme system in food and drinking water."

Foodborne bacterial infection poses a serious threat to human health. As most diseases are caused by living bacteria, real-time assessment of bacterial viability is vitally important to the public health sector. Herein, we developed a simple and novel colorimetric assay based on the Glucose oxidase (GOD)/Horseradish peroxidase (HRP) bienzyme system for real-time monitoring of bacterial viability in food and drinking water. This bienzyme system is free of any chemical synthesis and only requires 3 sample handling steps. The color response is easily observable with the naked eye or recordable with a smartphone for precise determination of bacterial viability. The proposed strategy was validated with various bacteria both Gram-positive and Gram-negative, indicating its capability for broad-spectrum bacteria viability detection. Therefore, the proposed strategy shows promise for rapid and reliable quality control in food and drinking water.

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This article was published in the following journal.

Name: Food chemistry
ISSN: 1873-7072
Pages: 126581

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