Topics

Phenotypic Diversity Caused by Differential Expression of -Cre Transgenic Alleles.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Phenotypic Diversity Caused by Differential Expression of -Cre Transgenic Alleles."

Type II alveolar epithelial cells (AEC2s) play an essential role in the function and maintenance of the pulmonary epithelium. Several transgenic mice have been developed to study the function of these cells in vivo by using the human surfactant protein C (SFTPC) promoter to drive expression of Cre recombinase. The precise activity of each of these transgenic alleles has not been studied and previous reports suggest that their activity can depend on breeding strategies. We bred mice with a conditional allele of the essential telomere capping protein TRF2 with two different SFTPC-Cre transgenic strains and observed opposite phenotypes (100% lethality versus 100% viability). We characterized the Cre recombinase activity in these two transgenic lines and found that the contrasting phenotypes were driven by difference in embryonic expression of the two transgenes, likely due to position effects or differences in the transgenic constructs. We also tested if SFTPC-Cre activity was dependent on maternal or paternal inheritance. When paternally inherited, both SFTPC-Cre alleles produced offspring with constitutive reporter activity independent of the inheritance of the Cre allele, suggesting that Cre recombinase was expressed in the male germline prior to meiosis. Immunohistochemical analysis of the testis showed reporter activity during spermatogenesis. Analysis of single-cell RNA sequencing data from murine and human testis demonstrated SFPTC expression uniquely during human spermatogenesis, suggesting that use of the human promoter in these constructs is responsible for male germline activity. Our data highlight the importance of careful analysis of transgenic allele activity and identify an SFTPC-Cre allele that is useful for pan-epithelial targeting in the mouse.

Affiliation

Journal Details

This article was published in the following journal.

Name: American journal of respiratory cell and molecular biology
ISSN: 1535-4989
Pages:

Links

DeepDyve research library

PubMed Articles [20328 Associated PubMed Articles listed on BioPortfolio]

The confounding effects of high genetic diversity on the determination and interpretation of differential gene expression analysis in the parasitic nematode Haemonchus contortus.

Differential expression analysis between parasitic nematode strains is commonly used to implicate candidate genes in anthelmintic resistance or other biological functions. We have tested the hypothesi...

Differential gene expression between fungal mating types is associated with sequence degeneration.

Degenerative mutations in non-recombining regions, such as on sex chromosomes, may lead to differential expression between alleles if mutations occur stochastically in one or the other allele. Reduced...

Evolutionary genetics in insect phenotypic radiations: the value of a comparative genomic approach.

Expanding genome sequencing and transgenic technologies are enabling the discovery of genes driving phenotypic diversity across insect taxa. Limitations in downstream functional genetic approaches, ho...

Trade-offs between gene expression, growth and phenotypic diversity in microbial populations.

Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evol...

A shared genetic basis of mimicry across swallowtail butterflies points to ancestral co-option of doublesex.

Uncovering whether convergent adaptations share a genetic basis is consequential for understanding the evolution of phenotypic diversity. This information can help us understand the extent to which sh...

Clinical Trials [4698 Associated Clinical Trials listed on BioPortfolio]

Differential Expression of CD200 in B-chronic Lymphoproliferative Disorders by Multicolour Flow Cytometry

The investigator's goals in this study are to assess : 1. Differential expression of CD200 by using flow cytometric immune-phenotyping in broad range of patients with B-chronic lym...

Evaluating the Expression Levels of MicroRNA-10b in Patients With Gliomas

MicroRNAs (miRNA) are molecular biomarkers that post-transcriptionally control target genes. Deregulated miRNA expression has been observed in diverse cancers. In high grade gliomas, know...

Identification of microRNAs Involved in Cerebral Collateral Regulation

Patients >18 years of age presenting to Ohio State Wexner Medical Center Emergency department with stroke symptoms, within 6 hours of last know well and found to have acute anterior circul...

Vancomycin Associated Red Man Syndrome (RMS)

This study proposes to identify patients who developed RMS with vancomycin infusion, and determine presence or absence of variant alleles involved in histamine biotransformation. The impli...

DNA Typing of HLA-DR/DQ Alleles in Taiwan Chinese With Rheumatic Heart Disease

The current study was designed to determine whether the HLA-DRB1, DQA1, DQB1 alleles are associated with the risk and the pattern of valve damage of RHD.

Medical and Biotech [MESH] Definitions

The different ways GENES and their ALLELES interact during the transmission of genetic traits that effect the outcome of GENE EXPRESSION.

The loss of one allele at a specific locus, caused by a deletion mutation; or loss of a chromosome from a chromosome pair, resulting in abnormal hemizygosity. It is detected when heterozygous markers for a locus appear monomorphic because one of the ALLELES was deleted. When this occurs at a tumor suppressor gene locus where one of the alleles is already abnormal, it can result in neoplastic transformation (CELL TRANSFORMATION, NEOPLASTIC).

Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.

Vaccines or candidate vaccines derived from edible plants. Transgenic plants (PLANTS, TRANSGENIC) are used as recombinant protein production systems and the edible plant tissue functions as an oral vaccine.

The capability of an organism to survive and reproduce. The phenotypic expression of the genotype in a particular environment determines how genetically fit an organism will be.

Quick Search


DeepDyve research library

Relevant Topics

Genetic Engineering
Genetic engineering is the process of manually adding new DNA to an organism. The goal is to add one or more new traits that are not already found in that organism. Examples of genetically engineered (transgenic) organisms currently on the market include...

Pulmonary
Pulmonary relating to or associated with the lungs eg Asthma, chronic bronchitis, emphysema, COPD, Cystic Fibrosis, Influenza,  Lung Cancer, Pneumonia, Pulmonary Arterial Hypertension, Sleep Disorders etc Follow and track Lung Cancer News ...

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...


Searches Linking to this Article