Expression of neovascular associated factors PEDF and αB-crystallin in human lens epithelial cells.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Expression of neovascular associated factors PEDF and αB-crystallin in human lens epithelial cells."

ABSTRACT : To investigate the expression of pigment epithelium-derived factor (PEDF) and αB-crystallin in human lens epithelial cells (LEC) and explore their relationships with diabetes.: Lens anterior capsules attached with LEC were collected from cataract surgeries in patients with or without diabetes, and grouped as following: non-diabetes mellitus (NDM) group, no diabetic retinopathy (NDR) group, non-proliferative diabetic retinopathy (NPDR) group and proliferative diabetic retinopathy (PDR) group. The expression of PEDF and αB-crystallin in all groups were determined by Western blot and immunofluorescence assay.: PEDF and αB-crystallin protein were both detected in LEC. PEDF was mainly distributed in the cytoplasm, whereas αB-crystallin was present in both cytoplasm and nucleus. The levels of PEDF protein and αB-crystallin protein in LEC were significantly increased with the appearance and aggravation of diabetic retinopathy (DR) (p<0.01).: The expression of PEDF and αB-crystallin protein are both positively correlated with the progression of DR, which may contribute to the regulation of iris neovascularization.


Journal Details

This article was published in the following journal.

Name: Current eye research
ISSN: 1460-2202


DeepDyve research library

PubMed Articles [27776 Associated PubMed Articles listed on BioPortfolio]

Site-Specific Rapid Deamidation and Isomerization in Human Lens αA-crystallin in vitro.

Recent studies have suggested that the isomerization/racemization of aspartate residues in proteins increases in aged tissues. One such residue is Asp151 in lens-specific αA-crystallin. Although many...

Stability of αB-crystallin gene expression in canine mammary gland neoplasms. Should it be considered as circulating tumor cell genetic marker?

αB-crystallin is a member of a small family of thermal shock proteins that protects cells from stress. Because of lack of its expression in peripheral blood leukocytes, it was proposed as a molecular...

A Molecular Dynamics Perspective to Identify Precursors to Aggregation in Human γS-Crystallin Unravels the Mechanism of Childhood Cataracts.

Despite the increasing health risk from infantile cataracts, identifying the mechanism of this disease remains a challenge due to lack of structural investigations using experimental and computational...

Protective role of hesperetin against posttranslational oxidation of tryptophan residue of human γD-crystallin: A molecular level study.

Crystallin proteins undergo various posttranslational modifications with aging of eye lens. Oxidation of tryptophan (Trp) residues of a major γ-crystallin namely human γD-crystallin (HGD) was found ...

Novel anti-angiogenic PEDF-Derived small peptides mitigate, choroidal neovascularization.

Abnormal migration and proliferation of endothelial cells (EC) drive neovascular retinopathies. While anti-VEGF treatment slows progression, pathology is often supported by decrease in intraocular pig...

Clinical Trials [9106 Associated Clinical Trials listed on BioPortfolio]

Carbidopa-levodopa in Neovascular AMD

From 3 large patient databases, patients diagnosed with AMD who have never taken levodopa(L-DOPA) containing medications have a mean age of diagnosis at 71 years. Patients who have been tr...

Glucose Trnsporter and PEDF in Psoriasis

Psoriasis is a chronic relapsing cutaneous immune mediated inflammatory disease(IMID). In which there are skin lesions characterized by erythema, thickness and scale formation with differe...

Ocular Comfort and the "Aging" Lens (ERBIUM)

The purpose of this trial is to evaluate the factors affecting comfortable contact lens wear and how those factors affect contact lens replacement frequency.

Effect of Contact Lens With Alginic Acid in Dry Eye Patients

Contact lens induced dry eyes leads to lens intolerance and discontinuation. Incidence of dry eye in contact lens wearer is significantly higher in Asian population compared to other ethni...

Safety and Dose-finding Study of DC-TAB in Healthy Subjects

The purpose of this study is to determine safety and appropriate dose of DC-TAB for selective immune tolerance induction in humans.

Medical and Biotech [MESH] Definitions

One of the subunits of alpha-crystallins. Unlike ALPHA-CRYSTALLIN B CHAIN the expression of ALPHA-CRYSTALLIN A CHAIN is limited primarily to the lens (LENS, CRYSTALLINE).

One of the alpha crystallin subunits. In addition to being expressed in the lens (LENS, CRYSTALLINE), alpha-crystallin B chain has been found in a variety of tissues such as HEART; BRAIN; MUSCLE; and KIDNEY. Accumulation of the protein in the brain is associated with NEURODEGENERATIVE DISEASES such as CREUTZFELDT-JAKOB SYNDROME and ALEXANDER DISEASE.

A subclass of crystallins that provides the majority of refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits.

A class of crystallins that provides refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Beta-crystallins are similar in structure to GAMMA-CRYSTALLINS in that they both contain Greek key motifs. Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits.

A type of crystallin that has been found in the lens (LENS, CRYSTALLINE) of certain species of VERTEBRATES. They are inactivated form of PHOSPHOPYRUVATE HYDRATASE.

Quick Search

DeepDyve research library

Relevant Topics

Diabetes is a lifelong condition that causes a person's blood sugar level to become too high. The two main types of diabetes are: type 1 diabetes type 2 diabetes In the UK, diabetes affects approximately 2.9 million people. There are a...

Ophthalmology is the branch of medicine that is devoted to the study and treatment of eye diseases. As well as mild visual defects correctable by lenses, ophthalmology is concerned with glaucoma, uveitis and other serious conditions affecting the eye, ...

Nephrology - kidney function
Nephrology is a specialty of medicine and pediatrics that concerns itself with the study of normal kidney function, kidney problems, the treatment of kidney problems and renal replacement therapy (dialysis and kidney transplantation). Systemic conditions...

Searches Linking to this Article