Variability in skeletal muscle fibre characteristics during repeated muscle biopsy sampling in human vastus lateralis.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Variability in skeletal muscle fibre characteristics during repeated muscle biopsy sampling in human vastus lateralis."

The percutaneous muscle biopsy procedure is an invaluable tool for characterizing skeletal muscle and capillarization. Little is known about methodological or biological variation stemming from the technique in heterogeneous muscle. Five muscle biopsies were taken from the vastus lateralis of a group of young men ( = 29, 22 ± 1 years) over a 96-h period. We investigated the repeatability of fibre distribution, indices of muscle capillarization and perfusion, and myofibre characteristics. No differences between the biopsies were reported in myofibre type distribution, cross-sectional area (CSA), and perimeter. Capillary-to-fibre perimeter exchange index and individual capillary-fibre contacts were unchanged with respect to the location of the muscle biopsy and index of capillarization. The variability in the sampling distribution of fibre type specific muscle CSA increased when fewer than 150 muscle fibres were quantified. Variability in fibre type distribution increased when fewer than 150 muscle fibres were quantified. Myofibre characteristics and indices of capillarization are largely consistent throughout the vastus lateralis when assessed via the skeletal muscle biopsy technique. Markers of muscle capillarization and perfusion were unchanged across multiple sites of the human vastus lateralis. Myofibre characteristics such as muscle cross-sectional area, perimeter, and fibre type distribution were also unchanged. Variation of muscle CSA was higher when fewer than 150 muscle fibres were quantified.


Journal Details

This article was published in the following journal.

Name: Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme
ISSN: 1715-5320
Pages: 1-8


DeepDyve research library

PubMed Articles [12816 Associated PubMed Articles listed on BioPortfolio]

Accumulation of Skeletal Muscle T-cells and the Repeated Bout Effect in Rats.

The purpose of this investigation was to characterize skeletal muscle T-cell accumulation following contraction-induced muscle damage, and test the hypothesis that T-cells contribute to post-damage mu...

3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice.

In obesity, the skeletal muscle capillary network regresses and the insulin-mediated capillary recruitment is impaired. However, it has been shown that in the early stage of advanced obesity, an incre...

Vascularized and Innervated Skeletal Muscle Tissue Engineering.

Volumetric muscle loss (VML) is a devastating loss of muscle tissue that overwhelms the native regenerative properties of skeletal muscle and results in lifelong functional deficits. There are current...

Impact of Skeletal Muscle Mass on Metabolic Health.

Skeletal muscle is regarded as an endocrine and paracrine organ. Muscle-derived secretory proteins, referred to as myokines, mediate interactions between skeletal muscle mass and other organs such as ...

Histological aspects of skeletal muscle fibers splitting of C57BL/6NCrl mice.

The objective of the current study is to present data on the splitting of skeletal muscle fibers in C57BL/6NCrl mice. Skeletal muscles (m. rectus femoris (m. quadriceps femoris)) from 500 (250 female ...

Clinical Trials [10486 Associated Clinical Trials listed on BioPortfolio]

Lower and Higher Load Resistance Exercise Protocols: Acute Muscle Activation and Skeletal Muscle Hypertrophy

How much weight an individual lifts per workout does not dictate the relative increase in muscle size the individual gains following weeks of training, which is contrary to current strengt...

Variability of the Unique Fiber Potentials of a Spastic Muscle Treated With Botulinum Toxin

The main objective is to study the variability and evolution of single-fiber jitter and fiber density (FD) electrophysiological parameters in a spastic muscle during botulinum toxin typa A...

Tissue Donation for Myoblast Culture

Analyze human skeletal muscle and learn more about the ability of adult muscle to produce myoblasts (young muscle cells).

Strength Training for Skeletal Muscle Adaptation After Stroke

Chronically disabled stroke survivors experience accelerated skeletal muscle atrophy and other detrimental changes to muscle and surrounding tissues on the paretic side. This unilateral ti...

A Phase 2 Study to Evaluate the Impact of MTP-131 (Bendavia™) on Skeletal Muscle Function in Elderly

This study will be a Phase 2, randomized, double-blind, placebo-controlled study, enrolling 45 elderly subjects with previous evidence of mitochondrial dysfunction to evaluate whether the ...

Medical and Biotech [MESH] Definitions

A subtype of mitochondrial ADP, ATP translocase found primarily in heart muscle (MYOCARDIUM) and skeletal muscle (MUSCLE, SKELETAL).

Abnormal increase in skeletal or smooth muscle tone. Skeletal muscle hypertonicity may be associated with PYRAMIDAL TRACT lesions or BASAL GANGLIA DISEASES.

Mature contractile cells, commonly known as myocytes, that form one of three kinds of muscle. The three types of muscle cells are skeletal (MUSCLE FIBERS, SKELETAL), cardiac (MYOCYTES, CARDIAC), and smooth (MYOCYTES, SMOOTH MUSCLE). They are derived from embryonic (precursor) muscle cells called MYOBLASTS.

Elongated, spindle-shaped, quiescent myoblasts lying in close contact with adult skeletal muscle. They are thought to play a role in muscle repair and regeneration.

Drugs used for their actions on skeletal muscle. Included are agents that act directly on skeletal muscle, those that alter neuromuscular transmission (NEUROMUSCULAR BLOCKING AGENTS), and drugs that act centrally as skeletal muscle relaxants (MUSCLE RELAXANTS, CENTRAL). Drugs used in the treatment of movement disorders are ANTI-DYSKINESIA AGENTS.

Quick Search

DeepDyve research library

Relevant Topics

Arthritis Fibromyalgia Gout Lupus Rheumatic Rheumatology is the medical specialty concerned with the diagnosis and management of disease involving joints, tendons, muscles, ligaments and associated structures (Oxford Medical Diction...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Searches Linking to this Article