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Melatonin Enhances the Development of Porcine Cloned Embryos by Improving DNA Methylation Reprogramming.

08:00 EDT 24th March 2020 | BioPortfolio

Summary of "Melatonin Enhances the Development of Porcine Cloned Embryos by Improving DNA Methylation Reprogramming."

Incomplete DNA methylation reprogramming in cloned embryos leads to poor cloning efficiency. Melatonin has been proven to improve the development of cloned embryos, however, the role of melatonin during somatic cell nuclear transfer remains unclear. This work demonstrated that 10 M melatonin significantly enhanced the developmental progress, reduced the arrested rate before zygotic genome activation, and upregulated the blastocyst rate of cloned embryos. Melatonin also promoted the pseudo-pronucleus formation, increased blastocyst cell number, and reduced embryo apoptosis through upregulating the expression of antiapoptosis factors while downregulating the transcription of proapoptosis genes. Further study displayed that DNA methylation reprogramming related genes were greatly improved in cloned embryos when treated with melatonin; then, melatonin effectively promoted genomic DNA demethylation and DNA remethylation, DNA demethylation of pluripotency related gene , DNA methylation maintenance of imprinted gene , and DNA remethylation of tissue-specific gene in cloned embryos. Thus, zygotic genome activation related gene , pluripotency related genes , , and , imprinted genes and , and blastocyst quality related genes and were remarkably upregulated, and tissue-specific genes and were considerably silenced. In conclusion, melatonin enhanced the development of cloned embryos by ameliorating DNA methylation reprogramming. This work reveals that melatonin can regulate DNA methylation reprogramming and provides a novel insight to improve cloning efficiency.

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Name: Cellular reprogramming
ISSN: 2152-4998
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Medical and Biotech [MESH] Definitions

A biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the PINEAL GLAND. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of SLEEP, mood, and REPRODUCTION. Melatonin is also an effective antioxidant.

A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.

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A melatonin receptor subtype primarily found expressed in the BRAIN and RETINA.

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