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PubMed Journals Articles About "Separations" - Page: 2 RSS

15:28 EST 11th November 2019 | BioPortfolio

Separations PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Separations articles that have been published worldwide.

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Showing "Separations" PubMed Articles 26–50 of 103

Mechanical Control of the Kinetic Propylene/Propane Separation by Zeolitic Imidazolate Framework-8.

One intriguing feature of many porous MOFs is their stimulus-induced flexibility that makes them unique compared to the standard adsorbents. Here we propose an innovative concept to achieve an efficient kinetic separation of species with similar properties via a mechanical fine-tuning of the pore architecture of the flexible Zeolitic Imidazolate Framework ZIF-8. This unprecedented approach was applied to one of the most challenging societally-relevant separations, the propylene/propane separation which is o...


Subminute micro-isolation of pharmaceuticals with ultra-high pressure liquid chromatography.

The drive for faster separations while maintaining quality and yield remains an important consideration for enhanced productivity as well as cost reduction for drug discovery laboratories in the pharmaceutical industry. High-throughput experimentation (HTE) and high-throughput screening (HTS) techniques can benefit from rapid and efficient isolation of product at high purity and recovery from microgram-scale crude reaction mixtures. In this study we describe the isolation of small molecule and biomolecule c...

Controlled Assembly of Block Copolymer Coated Nanoparticles in 2D Arrays.

The defined assembly of nanoparticles (NPs) in polymer matrices is an important prerequisite for next-generation functional materials. A promising approach to control NP positions in polymer matrices at the nanometer scale is the use of block copolymers. It allows the selective deposition of NPs in nanodomains, but the final defined and ordered positioning of the NPs within the domains has not been possible. This can now be achieved by coating NPs with block copolymers. The self-assembly of block copolymer-...


Advancement in the Biomedical Applications of the (Nano)gel Structures Based on Particular Polysaccharides.

(Nano)gels from macromolecular compounds-natural, synthetic, or a combination thereof, suitable crosslinkers-and conferred characteristics-such as degradability, size, charge, amphiphilicity, responsiveness, and softness-are capable of responding to the challenges imposed by bioengineering applications. Polysaccharide-based gels have received particular attention in this field. This review addresses recent advancement in the use of (nano)gel structures prepared only from compounds based on gellan gum, hepar...

Perception of the ethical acceptability of live prey feeding to aquatic species kept in captivity.

Previous research into public perceptions of live prey feeding has been focused on terrestrial animals. The reasons for this likely relate to the difficulty humans have in being compassionate to animals who are phylogenetically distantly related. In order to test these assumptions, the general public (two groups; one who had just visited an aquarium; and one group who had just visited a zoo), aquarium professionals in the UK/US and terrestrial zoo animal professionals (UK) were investigated to see how they ...

Lateral effects in pattern vision.

There is a large literature on lateral effects in pattern vision but no consensus about them or comprehensive model of them. This paper reviews the literature with a focus on the effects of parallel context in the central fovea. It describes seven experiments that measure detection and discrimination thresholds in annular and Gabor-pattern contexts at different separations. It presents a model of these effects, which is an elaboration of Foley's (1994) model. The model describes the results well, and it sho...

A bipartite Kronig-Penney model with Dirac-delta potential scatterers.

Here we present a simple extension to the age-old Kronig-Penney model, which is made to be bipartite by varying either the scatterer separations or the potential heights. In doing so, chiral (sublattice) symmetry can be introduced. When such a symmetry is present, topological chiral symmetry protected edge states are seen to exist in correspondence with the standard quantized Zak phase bulk invariant. This quantization behaviour may also be observed within a `gauge'-invariant on-diagonal matrix element of a...

Simultaneous separation and determination of seven isoflavones in Radix Puerariae by capillary electrophoresis with a dual cyclodextrin system.

A simple, comprehensive, and efficient capillary electrophoresis method using a dual cyclodextrin system was developed for the simultaneous determination of seven isoflavones (3'-methoxypuerarin, puerarin, 3'-hydroxypuerarin, ononin, daidzin, daidzein and genistin). Baseline separations of the seven isoflavones were achieved within 11 min with the running buffer consisting of 35 mM sodium tetraborate, 9.0 mM sulfobutylether-β-cyclodextrin and 30 mM α-cyclodextrin at pH 9.34, and peaks were detected at 254...

Chaotropic Effects in Supercritical Fluid Chromatography via Ammonium Hydroxide in Water-rich Modifiers: Enabling Separation of Peptides and Highly Polar Pharmaceuticals at Preparative Scale.

Chromatographic separation, analysis and characterization of complex highly polar analyte mixtures can often be very challenging using conventional separation approaches. Analysis and purification of hydrophilic compounds have been dominated by liquid chromatography (LC) and ion-exchange chromatography (IC), with supercritical fluid chromatography (SFC) moving towards these new applications, beyond traditional chiral separations. However, the low polarity of supercritical carbon dioxide (CO2) has limited th...

Designing Open Metal Sites in Metal-Organic Frameworks for Paraffin/Olefin Separations.

Incorporating open metal sites (OMS) into metal-organic frame-works allows design of well-defined binding sites for selective mo-lecular adsorption, which has profound impact on catalysis and sepa-rations. We demonstrate that Cu(I) sites incorporated into MFU-4l preferentially adsorb olefins over paraffins. DFT calculations show that the OMS are independent, with no dependence of binding energy on olefin loading up to one olefin per Cu(I). Experimentally, increasing Cu(I) loading increased olefin uptake wit...

Anionic trypsin from the spleen of albacore tuna (Thunnus alalunga): Purification, biochemical properties and its application for proteolytic degradation of fish muscle.

Anionic trypsin from albacore tuna spleen was purified by chromatographic separations on Q-Sepharose, Superdex 75 and Arginine Sepharose 4B. The trypsin migrated as single bands in both SDS-PAGE and native-PAGE. The molecular weight of purified trypsin was estimated to be 30 kDa using SDS-PAGE. The enzyme exhibited maximal activity at pH 9.0 and 55 °C for hydrolysis of Boc-Val-Pro-Arg-MCA. pH and temperature stabilities of the trypsin were well maintained in the pH range of 6-11 and over a temperatur...

Managing the column equilibration time in hydrophilic interaction chromatography.

For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial equilibration was demonstrated in gradient elution after purging with as little as 12 column volumes of mobile phase. Relative standard deviations of retention time of on average ~0.15% could be obtained after 1 or 2 conditioning (blank) runs. The equilibration period must be kept strictly constant, otherwise selectivity changes occur, but this is not problematic on modern instruments. Partial equilibration ...

Recent developments in capillary and microchip electroseparations of peptides (2017 - mid 2019).

The review presents a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography) for analysis, micropreparation, and physicochemical and biochemical characterization of peptides since 2017 up to ca. the middle of 2019. Progress in the study of electromigration properties of peptides and in the...

High-performance liquid chromatographic and subcritical fluid chromatographic separation of α-arylated ß-carboline, N-alkylated tetrahydroisoquinolines and their bioisosteres on polysaccharide-based chiral stationary phases.

New, pharmacologically interesting chiral amino compounds, namely stereoisomers of α-hydroxynaphthyl-ß-carboline, benz[d]azepine and benz[c]azepine analogs as well as N-α-hydroxynaphthylbenzyl-substituted isoquinolines were enantioseparated by high-performance liquid chromatographic and subcritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases. Separation of the stereoisomers was optimized in both SFC and normal phase liquid chromatographic (NP-LC) modalities by inv...

Strain-Guided Oxidative Nanoperforation on Graphene.

Increased applications of nanoporous graphene in nanoelectronics and membrane separations require ordered and precise perforation of graphene, whose scalablility and time/cost effectiveness represent a significant challenge in existing nanoperforation methods, such as catalytical etching and lithography. A strain-guided perforation of graphene through oxidative etching is reported, where nanopores nucleate selectively at the bulges induced by the prepatterned nanoprotrusions underneath. Using reactive molec...

Thermotropic Phase Transitions in Butyltrimethylammonium Bis(Trifluoromethylsulfonyl)Imide Ionic Liquids Are Dependent on Heat Flux.

Transmission Infrared Spectroscopy (IR) and Differential Scanning Calorimetry (DSC) are used to identify and characterize multiple phase transitions of butyltrimethylammonium bis(trifluoromethylsulfonyl)imide ([N1114] [TFSI]). Phase dependent properties are analyzed using temperature-controlled transmission IR, in which we identify changes in the molecular arrangements at and around phase transition temperatures between -80° C and 30° C, which span crystallization and melting phase transitions. Our data r...

Bistable colloidal orientation in polar liquid near a charged wall.

We examine the translation and rotation of an uncharged spheroidal colloid in polar solvents (water) near a charged flat surface. We solve the nonlinear Poisson-Boltzmann equation outside of the colloid in two dimensions for all tilt angles θ with respect to the surface normal. The colloid's size is assumed to be comparable to the Debye's length and hence field gradients are essential. The Maxwell stress tensor, including the ideal gas pressure of ions, is integrated over the colloid's surface to give the ...

Ultrathin carbon molecular sieve films and room-temperature oxygen functionalization for gas-sieving.

Inorganic membranes based on carbon molecular sieve (CMS) films hosting slit-like pores can yield a high molecular selectivity with a sub-angstrom resolution in molecular differentiation, and therefore are highly attractive for energy-efficient separations. However, the selective layer thickness of the state-of-the-art CMS membranes for gas separation is thicker than 1 µm, yielding low gas permeance. Also, there is no room-temperature functionalization route for the modification of the pore-size-distributi...

Rapid quantification of underivatized alloisoleucine and argininosuccinate using mixed-mode chromatography with tandem mass spectrometry.

Plasma elevations of the amino acids alloisoleucine and argininosuccinic acid (ASA) are pathognomonic for maple syrup urine disease and argininosuccinate lyase deficiency, respectively. Reliable detection of these biomarkers is typically achieved using methods with tedious sample preparations or long chromatographic separations, and many published amino acid assays report poor specificity and/or sensitivity for one or both of these compounds. This report describes a novel liquid chromatography tandem mass s...

Amphiphilic triptycene-based stationary phase for high-resolution gas chromatographic separations.

This work reports a new type of triptycene-based amphiphilic stationary phase (TP-2IL) for gas chromatography (GC). It is an integration of the 3D π-rich triptycene framework with ionic liquids. Its capillary column showed the efficiency of 3880 plates/m determined by n-dodecane at 120 °C (k = 2.79) and exhibited good performance for analytes from apolar to polar nature. Particularly, it has outstanding capability for resolving critical pairs of anilines and phenols with good peak shapes and shows d...

High-throughput bioassays using "dip-and-go" multiplexed electrospray mass spectrometry.

A multiplexed system based on inductive nanoelectrospray mass spectrometry (nESI-MS) has been developed for high-throughput screening (HTS) bioassays. This system combines inductive nESI and field amplification micro-electrophoresis to achieve a "dip-and-go" sample loading and purification strategy that enables nESI-MS based HTS assays in 96-well microtiter plates. The combination of inductive nESI and micro-electrophoresis makes it possible to perform efficient in situ separations and clean-up of biologica...

LC-ESI-MS/MS quantification of carnosine, anserine, and balenine in meat samples.

The histidine-containing imidazole dipeptide carnosine and its methylated analogs anserine and balenine are present at high concentrations in vertebrate tissues. Although the physiological functions of the imidazole dipeptides have not been elucidated yet, it has been suggested that they play significant biological roles in animals. Despite increasing interest, few studies have challenged the quantifications of carnosine, anserine, and balenine by a single HPLC run because they have similar retention times....

Development and validation of a simple and sensitive high resolution LC/MS method for determination of PF-04620110 in dog plasma: application to the pharmacokinetic study.

In this study, a more sensitive and reliable quantitative method based on ultra-high performance liquid chromatography coupled with Q-Exactive-Orbitrap-MS in full-mass scan was developed and validated for the determination of PF-04620110 in dog plasma. After protein precipitation with acetonitrile, the samples separations were carried out on an ACQUITY BEH C column with 1 mM ammonium acetate in water and acetonitrile containing 0.1% acetic acid as mobile phase, at a flow rate of 0.4 mL/min. The assay showed...

Individual differences in behavior and heart rate variability across the preweaning period in the domestic horse in response to stress.

The study of individual differences in behavior and physiology has attracted considerable interest among behavioral biologists. Important questions include how early in life such differences emerge and to what extent they remain stable across development. Due to the demanding nature of longitudinal studies, there is still a lack of information on this in mammals, especially in large, long-lived species. Our aim in this study was to look for stable individual differences in behavior and physiology during ear...

The Ultrafast and Continuous Fabrication of a Polydimethylsiloxane Membrane by Ultraviolet-induced Polymerization.

As the most promising biofuel, biobutanol is considered a competitive substitute of fossil fuels. Efficient separation of biobutanol from fermentation broth requires the application of membrane technologies. The commonly used polydimethylsiloxane (PDMS) membrane cannot meet the required demand due to its discontinuous and highly polluting thermal fabrication process. Herein, we proposed an ultraviolet (UV)-induced polymerization strategy and realize the ultrafast and continuous fabrication of the PDMS membr...


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