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PubMed Journals Articles About "Manipulating Mosquito Tolerance Arbovirus Control" - Page: 4 RSS

23:38 EST 21st November 2019 | BioPortfolio

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Showing "Manipulating Mosquito Tolerance Arbovirus Control" PubMed Articles 76–100 of 17,000+

Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management.

The Centers for Disease Control and Prevention, Arbovirus Reference Collection (ARC) contains viral isolates from both environmental and human sources that are maintained in the laboratory through passage in suckling mouse brain and/or vertebrate and invertebrate cell culture. There has been increased concern regarding the effect of mycoplasma contamination on virus growth and its impact on research and phenotypic analysis. Therefore, quality control testing of virus preparations has become a routine part o...


Vertical transmission of dengue virus in Aedes aegypti and its role in the epidemiological persistence of dengue in Central and Southern Mexico.

Aedes aegypti and Aedes albopictus are the main mosquito species responsible for dengue virus (DENV) transmission to humans in the tropical and subtropical regions of the world. The role of vertical transmission in the epidemiology of dengue and the maintenance of this arbovirus in nature during interepidemic periods remain poorly understood, and DENV vertical transmission could sustain the existence of virus reservoirs within Aedes populations.

The effectiveness of inspections on reported mosquito larval habitats in households: A case-control study.

Dengue is an arboviral disease that imposes substantial health and economic burdens across the globe. Vector control remains a key strategy in settings where Dengvaxia (a dengue vaccine) has not been licenced due to safety concerns and where mass immunization programmes are not cost-effective. Though inspections are used as part of arboviral disease control programmes, evidence of their impact on the entomological activity in households is sparse.


Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.

The yellow fever mosquito, Aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, Zika (ZIKV), and chikungunya viruses to humans. Control of Ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. The objectives of this study were to assess the abilit...

Evaluation of the Persistence of Three Larvicides Used To Control In Arapiraca, Northeastern Brazil.

The chemical control of the mosquito is a great challenge worldwide, since several populations of this species are already resistant to traditional insecticides, such as temephos. In Brazil, alternative larvicides, such as () and pyriproxyfen, have been used more recently. In this study we evaluated the persistence of pyriproxyfen (Sumilarv 0.5%G), 2 commercial formulations of (Vectobac WDG and Vectobac G), and temephos (Fersol 1G) under field and simulated field conditions with treatments exposed to sun...

Mapping Aedes aegypti (Diptera: Culicidae) and Aedes albopictus Vector Mosquito Distribution in Brownsville, TX.

Aedes mosquitoes are vectors of several emerging diseases and are spreading worldwide. We investigated the spatiotemporal dynamics of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) mosquito trap captures in Brownsville, TX, using high-resolution land cover, socioeconomic, and meteorological data. We modeled mosquito trap counts using a Bayesian hierarchical mixed-effects model with spatially correlated residuals. The models indicated an inverse relationship between temperature and mosquito trap count...

Over-expression of CmSOS1 confers waterlogging tolerance in Chrysanthemum.

Manipulating the level of expression of SOS1, a protein which regulates the movement and distribution of sodium ions, has been shown to enhance the salinity tolerance of a number of plant species, but its involvement in the response to hypoxia is less well established. Here, the contribution of the chrysanthemum homolog CmSOS1 on the expression of waterlogging tolerance has been explored. The over-expression of CmSOS1 improved the survival rate of plants exposed to waterlogging, while the performance of CmS...

Developmental and comparative perspectives on mosquito immunity.

Diseases spread by mosquitoes have killed more people than those spread by any other group of arthropod vectors and remain an important factor in determining global health and economic stability. The mosquito innate immune system can act to either modulate infection with human pathogens or fight off entomopathogens and increase the fitness and longevity of infected mosquitoes. While work remains towards understanding the larval immune system and the development of the mosquito immune system, it has recently...

Correlation between cephalic circumference at birth and ocular alterations in patients with microcephaly potentially associated with Zika Virus infection.

The Zika virus (ZIKV) is an arbovirus isolated for the first time in 1947 and transmitted to humans by the Aedes aegypti mosquito. In Brasil, it was first detected in May 2015. Since then, ZIKV has been identified as the etiological agent of acute exanthematous disease in Brasil, and Neuropediatricians of the Recife warned about an epidemic of microcephaly, and the Brazilian Ministry of Health confirmed the association between ZIKV and Congenital malformations and neurological syndromes. The eye, as an exte...

Microevolution or wide tolerance? Level of stress proteins in the beet armyworm Spodoptera eqigua hübner (Lepidoptera: Noctuidae) exposed to cadmium for over 150 generations.

The aim of this study was to investigate whether the cadmium tolerance developed in the beet armyworm Spodoptera exigua selected for over 150 generations may be related to synthesis of the stress proteins metallothioneins (Mts) and 70 kDa heat shock proteins (HSP70). To achieve this, six S. exigua strains (control, k), 150-generation Cd exposure strain (cd), and four 18-generation Cd exposure strains differing in Cd concentration (cd44, cd22, cd11, cd5) were reared. Stress protein level was measured in th...

Evolution-Driven Attenuation of Alphaviruses Highlights Key Glycoprotein Determinants Regulating Viral Infectivity and Dissemination.

Understanding the fundamental mechanisms of arbovirus transmission and pathogenesis is essential to develop strategies for treatment and prevention. We previously took an in vivo evolution-based approach and identified the chikungunya virus E1 glycoprotein residue 80 to play a critical role in viral transmission and pathogenesis. In this study, we address the genetic conservation and function of position 80 and demonstrate that this residue is a key determinant in alphavirus infectivity and dissemination t...

CRISPR-cas 9 directed genome engineering for enhancing salt stress tolerance in rice.

Crop productivity in rice is harshly limited due to high concentration of salt in the soil. To understand the intricacies of the mechanism it is important to unravel the key pathways operating inside the plant cell. Emerging state-of-the art technologies have provided the tools to discover the key components inside the plant cell for salt tolerance. Among the molecular entities, transcription factors and/or other important components of sensing and signaling cascades have been the attractive targets and the...

Insecticidal Effect of Extracts Against .

Of the various plant extracts from 10 plant species tested against larvae of in the laboratory, only extracts from exhibited larvicidal activity. A chloroform leaf extract of exhibited relatively high activity with a lethal concentration causing 50% mortality (LC) of 15.89 ppm, while chloroform and ethyl acetate extracts of fruits were 19.70 and 19.52 ppm, respectively. The chloroform fruit extract at 10 ppm reduced the hatchability of eggs by 20% whereas the chloroform leaf extract was found to be les...

Leaf inputs from invasive and native plants drive differential mosquito abundances.

Ecological impacts of invasive alien species can be unpredictable and simultaneously span multiple habitat types and taxonomic groups. Invasive alien plants can have particularly severe impacts, and plant inputs into aquatic environments can profoundly alter community composition of invertebrates, such as mosquitoes. Here, we examine larval mosquito colonisation of aquatic containers treated with leaves from four different terrestrial plants: the invasive tickberry Lantana camara, invasive guava Psidium gua...

A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area.

Novel tools are needed to reduce nuisance and risk of exotic arbovirus transmission associated with the colonization of temperate regions by Aedes albopictus. Incompatible Insect Technique (IIT) is a population suppression approach based on cytoplasmic incompatibility between males with manipulated endosymbionts and wild females. Here we present the results of the first field experiment in Europe to assess the capacity of an Ae. albopictus line (ARwP) deprived of its natural endosymbiont Wolbachia and trans...

Baked egg tolerance: is it possible to predict?

To assess the frequency of baked egg tolerance in IgE-mediated egg allergy patients through the oral food challenge and to assess the tolerance predictability of different skin prick tests, as well as specific serum IgE measurement to egg proteins.

Malaria transmission through the mosquito requires the function of the OMD protein.

Ookinetes, one of the motile and invasive forms of the malaria parasite, rely on gliding motility in order to establish an infection in the mosquito host. Here we characterize the protein PBANKA_0407300 which is conserved in the Plasmodium genus but lacks significant similarity to proteins of other eukaryotes. It is expressed in gametocytes and throughout the invasive mosquito stages of P. berghei, but is absent from asexual blood stages. Mutants lacking the protein developed morphologically normal ookinete...

First Record of From Saint Croix, United States Virgin Islands.

The first report of on Saint Croix, United States Virgin Islands (USVI), is confirmed. Adult and larval specimens were collected in 2018 and 2019 through adult surveillance and larval collections. Specimens were identified by microscopic methods, and a representative specimen was confirmed by DNA sequencing (mitochondrial cytochrome oxidase subunit I). Morphological features are reviewed and compared with , a species previously reported in the USVI. Notes are provided on the locations, collection methods,...

Safety, pharmacokinetics, and mosquito-lethal effects of ivermectin in combination with dihydroartemisinin-piperaquine and primaquine in healthy adult Thai subjects.

Mass administration of antimalarial drugs and ivermectin are being considered as potential accelerators of malaria elimination. The safety, tolerability, pharmacokinetics and mosquito-lethal effects of combinations of ivermectin, dihydroartemisinin-piperaquine, and primaquine were evaluated. Co-administration of ivermectin and dihydroartemisinin-piperaquine, resulted in increased ivermectin concentrations with corresponding increases in mosquito-lethal effect across all subjects. Exposure to piperaquine was...

Characterization and Whole Genome Sequencing of AR23, a Highly Toxic Bacillus thuringiensis Strain Isolated from Lebanese Soil.

The demand for sustainable and eco-friendly control methods of pests and insects is increasing worldwide. From this came the interest in Bacillus thuringiensis, an entomopathogenic bacterium capable of replacing chemical pesticides. However, the possibility of pests developing resistance to a particular strain may impair its use, and there is a need to identify novel strains of this species as potential commercial biopesticides. B. thuringiensis sv. israelensis is one of the most successful serovars, widely...

Infection mechanisms and putative effector repertoire of the mosquito pathogenic oomycete Pythium guiyangense uncovered by genomic analysis.

Pythium guiyangense, an oomycete from a genus of mostly plant pathogens, is an effective biological control agent that has wide potential to manage diverse mosquitoes. However, its mosquito-killing mechanisms are almost unknown. In this study, we observed that P. guiyangense could utilize cuticle penetration and ingestion of mycelia into the digestive system to infect mosquito larvae. To explore pathogenic mechanisms, a high-quality genome sequence with 239 contigs and an N50 contig length of 1,009 kb was g...

Mosquito-fungus interactions and antifungal immunity.

The mosquito immune system has evolved in the presence of continuous encounters with fungi that range from food to foes. Herein, we review the field of mosquito-fungal interactions, providing an overview of current knowledge and topics of interest. Mosquitoes encounter fungi in their aquatic and terrestrial habitats. Mosquito larvae are exposed to fungi on plant detritus, within the water column, and at the water surface. Adult mosquitoes are exposed to fungi during indoor and outdoor resting, blood and sug...

Robust Iterative Stimulation with Self-Antigens Overcomes CD8 T Cell Tolerance to Self- and Tumor Antigens.

The immune system adapts to constitutive antigens to preserve self-tolerance, which is a major barrier for anti-tumor immunity. Antigen-specific reversal of tolerance constitutes a major goal to spur therapeutic applications. Here, we show that robust, iterative, systemic stimulation targeting tissue-specific antigens in the context of acute infections reverses established CD8 T cell tolerance to self, including in T cells that survive negative selection. This strategy results in large numbers of circulat...

A Survey for in Delaware and the Virus-Positive Pool Rates of and for West Nile and Zika Viruses.

The introduction of Zika virus to the USA in 2015 engendered heightened interest in its known vectors. is the primary vector, with considered a potential secondary vector, together with several other possible marginal vectors. In Delaware, has been collected rarely, but no breeding populations were detected during past intensive statewide surveillance efforts. However, there is an abundance of statewide. Both species are container breeders and are peri-domestic-increasing the risk for virus transmission...

Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya.

The composition and structure of microbial communities associated with mosquitoes remain poorly understood despite their important role in host biology and potential to be harnessed as novel strategies for mosquito-borne disease control. We employed MiSeq sequencing of the 16S rRNA gene amplicons to characterize the bacterial flora of field-collected populations of Aedes mcintoshi and Aedes ochraceus, the primary vectors of Rift Valley fever (RVF) virus in Kenya. Proteobacteria (53.5%), Firmicutes (22.0%) a...


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