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PubMed Journals Articles About "Noblegen Achieves Self GRAS Affirmation Protein Rich Euglena" - Page: 5 RSS

01:51 EST 26th February 2020 | BioPortfolio

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Showing "Noblegen Achieves Self GRAS Affirmation Protein Rich Euglena" PubMed Articles 101–125 of 17,000+

Secreted modular calcium-binding proteins in pathophysiological processes and embryonic development.

Secreted modular calcium-binding proteins (SMOCs) are extracellular glycoproteins of the secreted protein, acidic, and rich in cysteine-related modular calcium-binding protein family and include two isoforms, SMOC1 and SMOC2, in humans. Functionally, SMOCs bind to calcium for various cell functions. In this review, we provided a summary of the most recent advancements and findings of SMOC1 and SMOC2 in development, homeostasis, and disease states.


Molecular Determinants of Complex Formation between DNA and the AT-rich Interaction Domain of BAF250a.

AT-rich interaction domain (ARID)-containing BAF250a protein is a central DNA binding subunit of the SWI/SNF chromatin remodeling complex. ARIDs are found in several eukaryotic proteins that play roles in different aspects of cellular physiology. However, despite their biological importance, ARIDs remain relatively uncharacterized for their dynamics and DNA binding. Here, we have probed the structure and DNA binding properties of BAF250a ARID. We show that the core BAF250a ARID interacts with DNA sequences ...

Kinetic study of the expression of genes related to hepatic steatosis, glucose and lipid metabolism and cellular stress during overfeeding in mule ducks.

Induced by overfeeding, hepatic steatosis is a process exploited for the "foie gras" production in mule ducks. To better understand the mechanisms underlying its development, we analysed the physiological responses of mule ducks overfed with corn for a duration of 11 days. A kinetic analysis of glucose and lipid metabolisms and cell protection mechanisms was performed on 96 males mule ducks during overfeeding with three sampling times (after the 4, the 12 and the 22 meal). Gene expression and protein analys...


Biowaste-source-dependent synthetic pathways of redox functional groups within humic acids favoring pentachlorophenol dechlorination in composting process.

Humic acids (HAs) can function as electron mediators for contaminants transformation in different environments. The humus respiration can facilitate pentachlorophenol (PCP) dechlorination during different biowastes composting. However, different characteristics of synthetic pathways of redox functional groups within HAs during different biowastes composting have never been characterized. Herein, we assessed the synthetic pathways of redox functional groups within HAs from protein-, lignocellulose-, and lign...

Platelet-rich Plasma Improves Healing of Pressure Ulcers as Objectively Assessed by Digital Planimetry.

The effectiveness of autologous platelet-rich plasma (PRP) in chronic wounds remains controversial.

PAQR9 modulates BAG6-mediated protein quality control of mislocalized membrane proteins.

Protein quality control is crucial for maintaining cellular homeostasis and its dysfunction is closely linked to human diseases. The post-translational protein quality control machinery mainly composed of BCL-2-associated athanogene 6 (BAG6) is responsible for triage of mislocalized membrane proteins (MLPs). However, it is unknown how the BAG6-mediated degradation of MLPs is regulated. We report here that PAQR9, a member of the Progesterone and AdipoQ receptor (PAQR) family, is able to modulate BAG6-mediate...

Zein Particles Stabilized Water-in-Water Emulsion as a Vehicle for Hydrophilic Bioactive Compound Loading of Riboflavin.

Vitamins and flavonoids are two kinds of essential trace bioactives which are prone to photodegradation during food processing and storage. In this study, a particle stabilized water-in-water (W/W) emulsion system composed of soy protein isolate (SPI) and guar gum (GG) was applied in loading riboflavin. Based on the significant binding affinity differences of SPI ( = 1.11 × 10 L mol) and GG ( = 9.00 × 10 L mol) to riboflavin, this hydrophilic and light-sensitive bioactive compound was loaded in SPI-rich d...

The translational functions of embryonic poly(A)-binding protein during gametogenesis and early embryo development.

Embryonic poly(A)-binding protein (EPAB) is an RNA-binding protein that binds to the poly(A) tails and AU-rich element at the 3' ends of messenger RNA (mRNAs). The main functions of EPAB are to protect stored mRNAs from undergoing deadenylation and subsequent degradation and to be involved in their translational regulation during spermatogenesis, oogenesis, and early embryogenesis. Following the first characterization of Epab in the Xenopus oocytes and early embryos, spatial and temporal expression and pote...

Ammonia formation over Pd/Rh three-way catalysts during lean-to-rich fluctuations: The effect of catalyst ageing, exhaust temperature, lambda and duration in rich conditions.

The formation of ammonia (NH3) as a by-product during the operation of a three-way catalyst (TWC) in a simulated exhaust stream was investigated using a commercially available Pd/Rh TWC under steady-state and lean/rich cycling conditions. Ion Molecular Reaction Mass Spectrometry (IMR-MS) was applied to determine NO, NO2 and NH3 concentrations at a time resolution of 0.6s. Catalyst ageing was shown to result in a significant increase in the amount of NH3 formed which has received limited attention in the lit...

The DNA-binding protein HTa from is an archaeal histone analog.

Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In archaea, histones are widespread but not universal: several lineages have lost histone genes. What prompted or facilitated these losses and how archaea without histones organize their chromatin remains largely unknown. Here, we elucidate primary chromatin architecture in an archaeon without histones, which harbours a HU family protein (HTa) that protects part of the genome ...

Reverse Hybrid Therapy Achieves a Similar Eradication Rate as Standard Hybrid Therapy for Helicobacter Pylori Infection.

Reverse hybrid therapy is a simplified hybrid treatment for H pylori infection. It achieves a higher eradication rate than standard triple therapy. This study aimed to compare the efficacies of reverse hybrid and hybrid therapies in the treatment of H pylori infection.

Extraction, structural and functional properties of Haematococcus pluvialis protein after pigment removal.

Protein from Haematococcus pluvialis (H. pluvialis) residues after pigment removal was prepared by alkaline extraction and acid precipitation. And the structural and functional properties of protein extracts were measured and analyzed. The effect of extraction conditions (liquid/solid ratio 10-20 mL/g), pH 10-12 and 25-45 °C (temperature) on the yield of H. pluvialis protein (HP) was carried out using Box-Behnken design. Under optimum extraction conditions: liquid/solid 20 mL/g, pH 11.5 and lower...

EMR achieves similar oncological outcomes as ESD for gastric neoplasia of <1cm, requiring less expertise, training and time.

Molecular Mechanism of RNA Recognition by Zinc-Finger Antiviral Protein.

Zinc-finger antiviral protein (ZAP) is a host antiviral factor that specifically restricts a wide range of viruses. ZAP selectively binds to CG-dinucleotide-enriched RNA sequences and recruits multiple RNA degradation machines to degrade target viral RNA. However, the molecular mechanism and structural basis for ZAP recognition of specific RNA are not clear. Here, we report the crystal structure of the ZAP N-terminal domain bound to a CG-rich single-stranded RNA, providing the molecular basis for its specif...

Direct Observation of Amorphous Precursor Phases in the Nucleation of Protein-Metal-Organic Frameworks.

Protein-metal-organic frameworks (p-MOFs) are a prototypical example of how synthetic biological hybrid systems can be used to develop next-generation materials. Controlling p-MOF formation enables the design of hybrid materials with enhanced biological activity and high stability. However, such control is yet to be fully realized due to an insufficient understanding of the governing nucleation and growth mechanisms in p-MOF systems. The structural evolution of p-MOFs was probed by cryo-transmission electro...

Expression of Rice Metallothionein Isoforms in Escherichia coli Enhances the Accumulation of Trivalent and Hexavalent Chromium.

Metallothioneins (MTs) are members of a family of low molecular weight and cysteine-rich proteins that are involved in heavy metal homeostasis and detoxification in living organisms. Plants have multiple MT types that are generally divided into four subgroups according to the arrangement of Cys residues.

Protein Domain Microarrays as a Platform to Decipher Signaling Pathways and the Histone Code.

Signal transduction is driven by protein interactions that are controlled by posttranslational modifications (PTM). Usually, protein domains are responsible for "reading" the PTM signal deposited on the interacting partners. Protein domain microarrays have been developed as a high throughput platform to facilitate the rapid identification of protein-protein interactions, and this approach has become broadly used in biomedical research. In this review, we will summarize the history, development and applicati...

Genetic counselors' comfort and knowledge of cancer risk assessment for transgender patients.

Transgender individuals are often their own health advocates, especially if seeking hormone therapies and gender-affirmation surgeries. While literature exists in the genetic counseling field that explores the relationship between genetic counselors and lesbian, gay, and bisexual patients, there is less research that directly addresses transgender patients. This study assessed cancer genetic counselors' education, knowledge, and comfort with transgender health issues, such as hormone therapies and gender af...

Multivesicular body sorting and the exosomal pathway are required for the release of rat hepatitis E virus from infected cells.

Recent reports have shown that rat hepatitis E virus (HEV) is capable of infecting humans. We also successfully propagated rat HEV into human PLC/PRF/5 cells, raising the possibility of a similar mechanism shared by human HEV and rat HEV. Rat HEV has the proline-rich sequence, PxYPMP, in the open reading frame 3 (ORF3) protein that is indispensable for its release. However, the release mechanism remains unclear. The overexpression of dominant-negative (DN) mutant of vacuolar protein sorting (Vps)4A or Vps4B...

Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap.

Hunting cycle of the carnivorous plant Venus flytrap (Dionaea muscipula Ellis) is comprised of mechanism for rapid trap closure followed by slow hermetical sealing and activation of gene expression responsible for digestion of prey and nutrient uptake. In the present study, we focus on the late phase of Venus's flytrap hunting cycle when mechanical stimulation of the prey ceases and is replaced by chemical cues. We used two nitrogen-rich compounds (chitin and protein) in addition to mechanostimulation to in...

Does atmospheric processing produce toxic Pb-containing compounds? A case study in suburban Beijing by single particle mass spectrometry.

A single particle aerosol mass spectrometry (SPAMS) was deployed to investigate the mixing state and chemical processing of Pb-rich particles in suburban Beijing. Based on a large dataset of mass spectra, Pb-rich particles were classified into Pb-O-Cl-N-S (55%), Pb-N (17%), Pb-N-S (15%), and Pb-EC (7%). Residual coal combustion, industrial activities, and meteorological conditions were identified as main factors regulating the variations of Pb-rich particles in the atmosphere. The highest abundance of the P...

Proteasome independent protein knockdown by small-molecule inhibitor for the undruggable lung adenocarcinoma.

Therapeutic target identification and corresponding drug development is a demanding task for the treatment of lung adeno-carcinoma, especially the most malignant proximal-proliferative subtype without druggable protein kinase mutations. Using a cell-SELEX-generated aptamer, we discovered a new tumor driver protein, leucine-rich pentatricopeptide repeat-containing protein (LRPPRC), which is specifically overexpressed in the most lethal subtype of lung adenocarcinoma. Tar-geted LRPPRC protein knockdown is a p...

Protein Type, Protein Dose, and Age Modulate Dietary Protein Digestion and Phenylalanine Absorption Kinetics and Plasma Phenylalanine Availability in Humans.

Dietary protein ingestion stimulates muscle protein synthesis by providing amino acids to the muscle. The magnitude and duration of the postprandial increase in muscle protein synthesis rates are largely determined by dietary protein digestion and amino acid absorption kinetics.

Integrity of dural closure after autologous platelet rich fibrin augmentation: an in vitro study.

Watertight closure of the dura mater is fundamental in neurosurgery. Besides the classical suturing techniques, a variety of biomaterials have been proposed as sealants. Platelet rich fibrin (PRF) is an autologous biomaterial which can readily be obtained through low-speed centrifugation of patient's own blood. It is rich in fibrin, growth factors, leucocytes and cytokines and has shown adhesive properties while promoting the physiological wound healing process. In this study, we investigated the effect of ...

Intrinsically disordered protein as carbon nanotube dispersant: How dynamic interactions lead to excellent colloidal stability.

The rich pool of protein conformations combined with the dimensions and properties of carbon nanotubes create new possibilities in functional materials and nanomedicine. Here, the intrinsically disordered protein α-synuclein is explored as a dispersant of single-walled carbon nanotubes (SWNTs) in water. We use a range of spectroscopic methods to quantify the amount of dispersed SWNT and to elucidate the binding mode of α-synuclein to SWNT. The dispersion ability of α-synuclein is good even with mild soni...


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