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PubMed Journal Database | Biofabrication RSS

09:28 EDT 27th May 2019 | BioPortfolio

The US National Library of Medicine and National Institutes of Health manage PubMed.gov which comprises of more than 29 million records, papers, reports for biomedical literature, including MEDLINE, life science and medical journals, articles, reviews, reports and  books.

BioPortfolio aims to cross reference relevant information on published papers, clinical trials and news associated with selected topics - speciality.

For example view all recent relevant publications on Epigenetics and associated publications and clincial trials.

Showing PubMed Articles 1–25 of 119 from Biofabrication

Fabrication of size-controllable human mesenchymal stromal cell spheroids from micro-scaled cell sheets.

Recently, stem cell spheroids have been actively studied for use in tissue regeneration. In this study, we report a method for the fabrication of size-controllable stem cell spheroids in different sizes from micro-scaled cell sheets (μCS) using thermosensitive hydrogels and investigated their effects on stem cell function. Mesenchymal stem cells isolated from different tissues such as human turbinate tissue, bone marrow, and adipose tissue were adhered selectively to each micro-pattern (squares with widths...

A 3D construct of the intestine canal with wrinkle morphology on a centrifugation configuring microfluidic chip.

A new in vitro gut microfluidic chip that mimics the in vivo intestine canal morphology and stimulation is developed to contribute to tissue engineering, intestine development and function research. This strategy utilizes centrifugation to configure the spatial cells along with the side wall of a vertical cylinder-like microfluidic chamber, by which a tubular intestine epithelium cell sheet is formed. Diverse intestine cell lines are inoculated to address this approach. Furthermore, to generate a microenvir...

Fabrication of elastomer pillar arrays with elasticity gradient for cells migration, elongation and patterning.

The elasticity of the cell and that of the supporting ECM in tissue are correlated. In some cases, the modulus of the ECM varies with a high spatial gradient. To study the effect of such a modulus gradient on the cell culture behavior, we proposed a novel yet straightforward method to fabricate elastomeric micropillar substrates with different height gradients, which could provide a large range of elasticity gradient from 2.4 kPa to 60 kPa. The micropillars were integrated into a microfluidic chip to demons...

Multiscale hierarchical bioresorbable scaffolds for the regeneration of tendons and ligaments.

Lesions of tendons and ligaments account for over 40% of the musculoskeletal lesions. Surgical techniques and materials for repair and regeneration are currently not satisfactory. The high rate of post-operative complications and failures mainly relates to the technical difficulties in replicating the complex multiscale hierarchical structure and the mechanical properties of the native tendons and ligaments. With the aim of overcoming the limitations of non-biomimetic devices, we developed a hierarchical st...

Comprehensive tuning of bioadhesive properties of polydimethylsiloxane (PDMS) membranes with controlled porosity.

Polydimethylsiloxane (PDMS)-based elastomers have become the de facto platform for various biomedical applications. But the stable attachment of biomolecules to PDMS for more robust and long-term performance of the PDMS-based devices has been a significant challenge, owing to its unique physical properties (e.g. hydrophobicity, dynamic molecular mobility). Herein, the PDMS membrane with tunable surface porosity is developed via high-pressure saturated steam technology in order to promote a strong and lastin...

4D anisotropic skeletal muscle tissue constructs fabricated by staircase effect strategy.

Like the morphology of native tissue fiber arrangement (such as skeletal muscle), unidirectional anisotropic scaffolds are highly desired as a means to guide cell behavior in anisotropic tissue engineering. In contrast, contour-like staircases exhibit directional topographical cues and are judged as an inevitable defect of fused deposition modeling (FDM). In this study, we will translate this staircase defect into an effective bioengineering strategy by integrating FDM with surface coating technique (FCT) t...

Achieving molecular orientation in thermally extruded 3D printed objects.

 
 3D printing is used to fabricate tissue scaffolds. The polymer chains in these objects are typically unoriented. The mechanical properties of these scaffolds can be significantly enhanced by proper alignment of the polymer chains. But, post-processing routes to increase orientation can be limited by the geometry of the printed object. Here we show that it is possible to orient the polymer chains during printing by optimizing the printing parameters to take advantage of the flow characteristics ...

Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering.

The microenvironments of tissues or organs are complex architectures comprised of structural proteins including collagen. Particularly, the cornea is organized in a lattice pattern of collagen fibrils which play a significant role in its transparency. This paper introduces a transparent bioengineered corneal structure for transplantation. The structure is fabricated by inducing shear stress to a corneal stroma-derived decellularized extracellular matrix bioink based on 3D cell-printing technique. The printe...

Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks.

Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, lack of functionality can often be attributed to poor bioink properties. Indeed, advanced bioinks encapsulating living cells should: (i) present optimal rheological properties and retain 3D structure post-fabrication, (ii) promote cell viability and support cell differentiatio...

Engineering three-dimensional microenvironments towards in vitro disease models of the central nervous system.

The central nervous system (CNS) has a highly complex biophysical and biochemical environment. Despite decades of intensive research, it is still an enormous challenge to restore its functions and regenerate lost or damaged CNS tissues. Current treatment strategies remain sub-optimal because of (1) the hostile microenvironment created post CNS injury, and (2) insufficient understanding of the pathophysiology of acute and chronic CNS diseases. Two-dimensional (2D) in vitro models have provided tremendous ins...

Electrodeposition-based rapid bioprinting of 3D-designed hydrogels with a pin art device.

Three-dimensional (3D) designed hydrogels are receiving considerable attention for use in tissue engineering. Herein, we present a novel method for bioprinting 3D hydrogels by electrodeposition with a pin art device. The device consists of a metal substrate and an array of electrode pins that can slide independently. To fabricate a 3D hydrogel, pins are pushed from the rear with a 3D object to generate a 3D extruded-pin relief of the object; the extruded pins are then inserted into a chitosan/gelatin hydrog...

Engineering small-caliber vascular grafts from collagen filaments and nanofibers with comparable mechanical properties to native vessels.

At the present time, there is no successful synthetic, off-the-shelf small-caliber vascular graft (< 6 mm) for the repair or bypass of the coronary or carotid arteries. This stimulates on-going investigations to fabricate an artificial vascular graft that has both sufficient mechanical properties as well as superior biological performance. Collagen has long been considered as a viable material to encourage cell recruitment, tissue regeneration, and revascularization, but its use has been limited by its i...

Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering.

In current study, a novel strategy of cryogenic 3D bioprinting assisted by free-from extrusion printing was developed and applied to printing of a decellularized small intestinal submucosa (dSIS) slurry. The rheological properties, including kinetic viscosity, storage modulus (G') and loss modulus (G''), were appropriate for free-from extrusion printing of dSIS slurry. Three different groups of scaffolds, including P<sub>500</sub>, P<sub>600</sub>, and P<sub>700</sub>, wi...

3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering.

One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while - despite its key role in the osteochondral region - only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing ß-tricalcium phosphate (TCP) for engineerin...

3D bioprinting of triphasic nanocomposite hydrogels and scaffolds for cell adhesion and migration.

In this study we describe the first example of 3D (bio)printed triphasic chiral nanocomposite (NC) hydrogels/scaffolds to simulate the complex 3D architecture, nano/micro scale topography, and chiral nature of ECM. These multifunctional constructs are prepared using a 3D (bio)printing technique and are composed of three connected hydrogels/scaffolds, two of which are loaded with nanomaterials functionalized with opposite enantiomers of a biomolecule. With these constructs, we direct the migration of cells t...

Ultrasound-assisted biofabrication and bioprinting of preferentially aligned three-dimensional cellular constructs.

A critical consideration in tissue engineering is to recapitulate the microstructural organization of native tissues that is essential to their function. Scaffold-based techniques have focused on achieving this via the contact guidance principle wherein topographical cues offered by scaffold fibers direct migration and orientation of cells to govern subsequent cell-secreted extracellular matrix organization. Alternatively, approaches based on acoustophoretic, electrophoretic, photophoretic, magnetophoretic,...

3D printed dual macro-, microscale porous network as a tissue engineering scaffold with drug delivering function.

Tissue engineering macroporous scaffolds are important for regeneration of large volume defects resulting from diseases such as breast or bone cancers. Another important part of the treatment of these conditions is adjuvant drug therapy to prevent disease recurrence or surgical site infection. In this study, we developed a new type of macroporous scaffolds that have drug loading and release functionality to use in these scenarios. 3D printing allows for building macroporous scaffolds with deterministically ...

Recellularization of auricular cartilage via elastase-generated channels.

Decellularized tissue matrices are promising substrates for tissue generation by stem cells to replace poorly regenerating tissues such as cartilage. However, the dense matrix of decellularized cartilage impedes colonisation by stem cells. Here, we show that digestion of elastin fibre bundles traversing auricular cartilage creates channels through which cells can migrate into the matrix. Human chondrocytes and bone marrow-derived mesenchymal stromal cells efficiently colonize elastin-treated scaffolds throu...

Multi-level customized 3D printing for autogenous implants in skull tissue engineering.

Three-dimensional (3D) printing of decellularized extracellular matrix (ECM) has been achieved to ensure real physiological environments for tissue engineering. However, limited source, biocompatibility and biosafety of decellularized ECM are deficiencies in its large clinical use. Autogenous ECM is biocompatible, bioactive and bio-safe, making it an optimal choice for future clinical applications of 3D printing. Here, we developed a multi-level customized 3D printing (MLC-3DP) strategy applying autogenous ...

Modeling neural circuit, blood-brain barrier, and myelination on a microfluidic 96 well plate.

Microfluidics have enabled a wide range of experimental possibilities in the field of neuroscience. Unfortunately, the wider scale adoption of polydimethylsliloxane (PDMS) based microfluidic devices faces challenges due to inherent material compatibility issues and lack of standardized manufacturable devices. In this work, we present an injection molded plastic array 3-dimensional (3D) neuron culture platform (Neuro-IMPACT) made of polystyrene (PS) with a standard 96-well plate form factor that can recapitu...

Extrusion-based printing of sacrificial Carbopol ink for fabrication of microfluidic devices.

Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimethylsiloxane (PDMS) devices with resin biocompatibility, throughput and transparency for imaging. It is a multi-step process requiring the preparation of a silicon wafer pattern, which is fabrica...

Biomimetic design and fabrication of scaffolds integrating oriented micro-pores with branched channel networks for myocardial tissue engineering.

The ability to fabricate three-dimensional (3D) thick vascularized myocardial tissue could enable scientific and technological advances in tissue engineering and drug screening, and may accelerate its application in myocardium repair. In this study, we developed a novel biomimetic scaffold integrating oriented micro-pores with branched channel networks to mimic the anisotropy and vasculature of native myocardium. The oriented micro-pores were fabricated using an "Oriented Thermally Induced Phase Separation ...

Structural mechanics of 3D-printed poly(lactic acid) scaffolds with tetragonal, hexagonal and wheel-like designs.

While various porous scaffolds have been developed, the focused study about which structure leads to better mechanics is rare. In this study, we designed porous scaffolds with tetragonal, hexagonal and wheel-like structures under a given porosity, and fabricated corresponding poly(lactic acid) (PLA) scaffolds with three-dimensional (3D) printing. High-resolution micro-computed tomography (micro-CT) was carried out to calculate their experimental porosity and confirm their high interconnectivity. The theoret...

Femtosecond laser induced densification within cell-laden hydrogels results in cellular alignment.

The unique capabilities of ultrafast lasers to introduce user-defined microscale modifications within 3D cell-laden hydrogels have been used to investigate fundamental cellular phenomenon such as adhesion, alignment, migration and organization. In this work, we report a new material modification phenomenon coined as 'densification' and its influence on the behavior of encapsulated cells. Femtosecond laser writing technique was used to write densified lines of width 1-5μm within the bulk of gelatin methacry...

3D printing of complex GelMA-based scaffolds with nanoclay.

Photo-crosslinkable gelatin methacrylate (GelMA) has become an attractive ink in 3D printing due to its excellent biological performance. However, limited by low viscosity and long cross-linking time, it is still a challenge to directly print GelMA by extrusion-based 3D printing. Here, to balance the printability and biocompatibility, biomaterial ink composed of GelMA and nanoclay was specially designed. Using this ink, complex scaffolds with high shape fidelity can be easily printed based on the thixotropi...


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