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PubMed Journal Database | Biosensors & bioelectronics RSS

10:00 EDT 27th May 2019 | BioPortfolio

The US National Library of Medicine and National Institutes of Health manage PubMed.gov which comprises of more than 29 million records, papers, reports for biomedical literature, including MEDLINE, life science and medical journals, articles, reviews, reports and  books.

BioPortfolio aims to cross reference relevant information on published papers, clinical trials and news associated with selected topics - speciality.

For example view all recent relevant publications on Epigenetics and associated publications and clincial trials.

Showing PubMed Articles 1–25 of 1,900+ from Biosensors & bioelectronics

Graphene-based nanocomposites for sensitivity enhancement of surface plasmon resonance sensor for biological and chemical sensing: A review.

Surface plasmon resonance (SPR) offers exceptional advantages such as label-free, in-situ and real-time measurement ability that facilitates the study of molecular or chemical binding events. Besides, SPR lacks in the detection of various binding events, particularly involving low molecular weight molecules. This drawback ultimately resulted in the development of several sensitivity enhancement methodologies and their application in the various area. Among graphene materials, graphene-based nanocomposites s...

Photoelectrochemical biosensor of HIV-1 based on cascaded photoactive materials and triple-helix molecular switch.

In this work, an ultrasensitive photoelectrochemical (PEC) biosensor was proposed to detect nucleic acids on the basis of cascaded photoactive materials and triple-helix molecular switch. DNA sequence of human immunodeficiency virus type 1 (HIV-1) was chosen as the target DNA (T-DNA). Cascaded photoactive structure was formed via different sizes of CdTe quantum dots (QDs) sensitized ZnO nanorods (ZnO NRs), which was employed as a cascaded photoactive interface to amplify the photocurrent signal. A hairpin s...

Amperometric glucose biosensing performance of a novel graphene nanoplatelets-iron phthalocyanine incorporated conducting hydrogel.

Herein, a novel one step synthesis of multicomponent three dimensional polyacrylic acid (PAA) based conducting hydrogel (CH) incorporated with iron phthalocyanine functionalised graphene nanoplatelets (GPL-FePc) is reported. An amperometric glucose biosensor was fabricated by the immobilization of glucose oxidase (GOx) onto the synthesised PAA-VS-PANI/GPL-FePc-CH (where VS-PANI is vinyl substituted polyaniline). Scanning electron microscopy reveals the presence of three dimensional microporous structure wit...

Hierarchical Cu@CuO nanowires arrays-coated gold nanodots as a highly sensitive self-supported electrocatalyst for L-cysteine oxidation.

The sensitivity, selectivity, and stability of an electrochemical sensor for detecting small biomolecules can be significantly upgraded through properly controlling the morphology and chemical structure of electrocatalyst. Herein, we fabricated a unique hierarchical nanostructure based on Cu@CuO nanowires (NWs) array uniformly depositing with a layer of gold nanoparticles (2-3 nm) through a simple electroless deposition process. The Au-Cu@CuO NWs hybrid was successfully applied as a novel binder-free self...

Electrochemiluminescence for the identification of electrochemically active bacteria.

Electrochemically active bacteria (EAB) use extracellular electron transfer (EET) to exchange electron with extracellular acceptors. Previous studies regarding the measurement of EAB were based on either extracellular reduction or oxidation. In this work, we developed a simple electrochemiluminescence (ECL) assay for the identification and detection of EAB. The results of this proposed method revealed that EET of EAB influenced the content of dissolved oxygen and the formation of Ru(bpy) thus leading to qua...

Towards pharmacological treatment screening of cardiomyocyte cells using Si nanowire FETs.

Silicon nanowires (Si NWs) are the most promising candidates for recording biological signals due to improved interfacing properties with cells and the possibility of high-speed transduction of biochemical signals into detectable electrical responses. The recording of extracellular action potentials (APs) from cardiac cells is important for fundamental studies of AP propagation features reflecting cell activity and the influence of pharmacological substances on the signal. We applied a novel approach of usi...

On-chip anticancer drug screening - Recent progress in microfluidic platforms to address challenges in chemotherapy.

There is an increasing need for advanced and inexpensive preclinical models to accelerate the development of anticancer drugs. While costly animal models fail to predict human clinical outcomes, in vitro models such as microfluidic chips ('tumor-on-chip') are showing tremendous promise at predicting and providing meaningful preclinical drug screening outcomes. Research on 'tumor-on-chips' has grown enormously worldwide and is being widely accepted by pharmaceutical companies as a drug development tool. In l...

Suspended CNT-Based FET sensor for ultrasensitive and label-free detection of DNA hybridization.

A suspended carbon nanotube (SCNT)-based field effective transistor (SCNT-FET), which was fabricated by utilizing the surface tension of liquid silver to suspend a CNT between two Pd electrodes, was proposed for the detection of DNA hybridization. Benefits from the separation between the CNT and the substrates could be observed; namely, the conductivity of a SCNT-FET was much higher (two orders of magnitude) than that of a FET based on an unsuspended CNT and about 50% sensing surface of CNT was freed from s...

In situ terminus-regulated DNA hydrogelation for ultrasensitive on-chip microRNA assay.

In this work, a dynamic terminus-regulated fabric of DNA hydrogel was invented in debt to the reiterative catalysis of terminal deoxynucleotidyl transferase (TdT). It extended free 3'-OH end to an overhang of homopolymeric adenosine base pair, and alternated with branching from the frayed complementary seed oligo TG. The cycle of this template-independent and isothermal amplification resulted in a microscale dendritic DNA fractal at first, which then gelatinized into a cohesive and intricate 3D network. Det...

Red-emitting FIT-PNAs: "On site" detection of RNA biomarkers in fresh human cancer tissues.

To date, there are limited approaches for the direct and rapid visualization (on site) of tumor tissues for pathological assessment and for aiding cytoreductive surgery. Herein, we have designed FIT-PNAs (forced-intercalation-peptide nucleic acids) to detect two RNA cancer biomarkers. Firstly, a lncRNA (long noncoding RNA) termed CCAT1, has been shown as an oncogenic lncRNA over-expressed in a variety of cancers. The latter, an mRNA termed KRT20, has been shown to be over-expressed in metastases originating...

The construction of Mycobacterium tuberculosis 16S rDNA MSPQC sensor based on Exonuclease III-assisted cyclic signal amplification.

Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) remains one of the most serious infectious diseases all over the world. The key to reduce the spread and mortality rate of tuberculosis is to develop faster and more sensitive approaches for detection of M. tuberculosis. However, current detection methods can not meet the requirements of rapid clinical M. tuberculosis detection in terms of detection time. Herein, a new 16S rDNA multichannel series piezoelectric quartz crystal (MSPQC) sensor...

A supersensitive MSPQC bacterium sensor based on 16S rRNA and "DNA-RNA switch".

The early detection of bacterium plays a significant role in addressing serious public health issues. In this paper, a supersensitive multichannel series piezoelectric quartz crystal (MSPQC) sensor of bacterium based on 16S rRNA and "DNA-RNA switch" was constructed. The fragment in specific region of 16S rRNA was used as the biomarker of bacterium to ensure high specificity and to achieve the accurate judgment of microbial vitality. "DNA-RNA switch" was designed to conduct two electrodes by switching insula...

Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity.

A ratiometric electrochemical biosensor was constructed to detect telomerase activity based on electrocatalysis of cerium-based metal-organic frameworks (CeMOFs) and conformation switch of hairpin DNA. First, the CeMOFs were synthesized using Ce as nodes and 1,3,5-benzenetricarboxylic acid as linker in a green method, and then functionalized with gold nanoparticles. The resulted Au@CeMOF tags demonstrated an excellent electrocatalysis toward hydroquinone oxidation. Meanwhile, a methylene blue (MB) modified ...

A versatile dynamic light scattering strategy for the sensitive detection of microRNAs based on plasmonic core-satellites nanoassembly coupled with strand displacement reaction.

A low-cost, effective and enzyme-free sensing strategy for ultrasensitive microRNA (miRNA) detection was developed based on dynamic light scattering (DLS) coupled with strand displacement reaction (SDR). The combination of DLS and SDR was used to assess the size changes of core-satellites nanoassembly. This strategy realized the limit of detection (LOD) as low as 0.24 pM (S/N = 3) and the detection range of 5 pM-150 pM, which might urge this strategy as an ideal candidate for the sensitive detection of ...

A probeless and label-free electrochemical immunosensor for cystatin C detection based on ferrocene functionalized-graphene platform.

A novel electrochemical sensor with inherent redox activity mediated by ferrocene for Cystatin C (CysC), an early kidney failure biomarker, is described. The current response was mediated by graphene oxide-ferrocene nanofilm with redox-activity coming from electroactive species surface-confined. Anti-CysC antibodies were immobilized by their Fc portions on the drop-casting polyethyleneimine (PEI) film for improving the sensitivity and reproducibility. Stepwise modifications of the nanostructured surface wer...

Specific cell capture and noninvasive release via moderate electrochemical oxidation of boronic ester linkage.

Early diagnosis and therapy of cancer metastasis are of great importance for disease outcome. Circulating tumor cells (CTCs) offer the ability for noninvasive tumor profiling in real time. However, simply capturing and counting tumor cells are inadequate to provide valuable information about tumor. Efficiently releasing the captured cells is necessary for the downstream characterization. Herein, we describe a mild electrochemical strategy to effectively isolate CTCs from the bloodstream and rapidly release ...

MyoRobot 2.0: An advanced biomechatronics platform for automated, environmentally controlled skeletal muscle single fiber biomechanics assessment employing inbuilt real-time optical imaging.

We present an enhanced version of our previously engineered MyoRobot system for reliable, versatile and automated investigations of skeletal muscle or linear polymer material (bio)mechanics. That previous version already replaced strenuous manual protocols to characterize muscle biomechanics properties and offered automated data analysis. Here, the system was further improved for precise control over experimental temperature and muscle single fiber sarcomere length. Moreover, it also now features the calcul...

A label-free PFP-based photoelectrochemical biosensor for highly sensitive detection of PARP-1 activity.

Poly(ADP-ribose) polymerase-1 (PARP-1), as an original tumor marker, has aroused wide attention in recent years. However, only a few researches have been done for PARP-1 activity detection because PARP-1 is lack of optical or electrochemical property. In this work, a label-free and high-sensitive photoelectrochemical (PEC) biosensor for PARP-1 activity detection based on poly[9,9-bis(6'-N,N,N-trimethylammonium)hexyl]fluorenylene phenylene (PFP) has been designed. To the best of our knowledge, it is the firs...

A novel disposable self-adhesive inked paper device for electrochemical sensing of dopamine and serotonin neurotransmitters and biosensing of glucose.

In this work, we detail the progress of a novel electrochemical disposable device, which has a relatively low cost and easy production, with a novel conductive ink, that consists of graphite and automotive varnish mixture, deposited over a self-adhesive paper, granting an easy production with relatively low cost. The electrode surface was characterized by scanning electron microscopy, X-ray powder diffraction and Fourier transforms infrared and Raman, cyclic voltammetry and electrochemical impedance spectro...

Novel coreactant modifier-based amplified electrochemiluminescence sensing method for point-of-care diagnostics of galactose.

Herein, an innovative coreactant modifier-based amplified ECL sensing method was developed to provide a convenient and effective amplified ECL emission. Firstly, nitrogen-rich quantum dots (N-dots) were prepared. N-dots have much nitrogen functional groups, which provide more reactive sites on the surface in the ECL reaction. Furthermore, we investigated the action mechanism of HO as coreactant modifier to promote the generation of more oxidant of coreactant in the N-dot/KSO coreactant ECL pathway. The core...

A ratiometric fluorescent assay for the detection and bioimaging of alkaline phosphatase based on near infrared AgS quantum dots and calcein.

Herein, a ratiometric fluorescent method was developed for alkaline phosphatase (ALP) detection based on near-infrared (NIR) AgS quantum dots (QDs) and calcein through the competitive approach. The system based on AgS QDs and calcein shows green (maximum emission at 512 nm from calcein) and near infrared (NIR) fluorescence (maximum 798 nm from AgS QDs) under the same excitation wavelength (468 nm). In the presence of Ce, the fluorescence intensity of calcein is decreased due to static quenching, while...

Recent progress in the design fabrication of metal-organic frameworks-based nanozymes and their applications to sensing and cancer therapy.

The nanomaterials with enzyme-like catalytic activity, named as nanozymes, have aroused wide research interest owing to their striking merits. Metal-organic frameworks (MOFs) have showed great prospect in the construction of novel nanozymes. In this review, firstly, we summarize the most recent contributions in the design construction of the MOFs-based nanozymes. Then, we concentrate our attention on their applications in the fields of sensing and cancer therapies. According to the design strategies, we cat...

Low-cost and user-friendly biosensor to test the integrity of mRNA molecules suitable for field applications.

The use of mRNA in biotechnology has expanded with novel applications such as vaccines and therapeutic mRNA delivery recently demonstrated. For mRNA to be used in patients, quality control assays will need to be routinely established. Currently, there is a gap between the highly sophisticated RNA integrity tests available and broader application of mRNA-based products by non-specialist users, e.g. in mass vaccination campaigns. Therefore, the aim of this work was to develop a low-cost biosensor able to test...

Single conical track-etched nanopore for a free-label detection of OSCS contaminants in heparin.

The heparin contamination by oversulfated chondroitin (OSCS) was at the origin of one major sanitary problem of last decade. Here we propose a novel strategy to detect OSCS from heparin solution based on conical nanopore functionalized with poly-L-lysine deposition to ensure its re-usability. This sensor is an excellent to detect low heparin concentration (from 25 ng/ml to 3 μg/ml) using the modification of ionic current rectification. It also allows following the kinetic of heparin degradation by hepa...

Prostate cancer biomarkers detection using nanoparticles based electrochemical biosensors.

Prostate cancer (PCa) is the most prevalent cancer with a high mortality rate. The early and accurate detection of PCa can significantly reduce mortality and saves lives. Hence, the nanomaterials based electrochemical nano-biosensors for PCa biomarkers will be the excellent alternative for diagnosis, detection and management of disease condition. In this review, we present a concise summary of the latest attainment and advancement in the use of nanoparticles for the diagnosis of PCa biomarkers. This review ...


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