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PubMed Journal Database | Biotechnology journal RSS

09:11 EDT 27th May 2019 | BioPortfolio

The US National Library of Medicine and National Institutes of Health manage PubMed.gov which comprises of more than 29 million records, papers, reports for biomedical literature, including MEDLINE, life science and medical journals, articles, reviews, reports and  books.

BioPortfolio aims to cross reference relevant information on published papers, clinical trials and news associated with selected topics - speciality.

For example view all recent relevant publications on Epigenetics and associated publications and clincial trials.

Showing PubMed Articles 1–25 of 271 from Biotechnology journal

Plasmid copy number of pTRKH3 in Lactococcus lactis is increased by modification of the repDE ribosome binding site.

Plasmids for DNA vaccination are exclusively produced in the Gram-negative Escherichia coli. One important drawback of this system is the presence of lipopolysaccharides. The Generally Recognized As Safe Lactococcus lactis would constitute a safer alternative for plasmid production. A key requirement for the establishment of a cost effective L. lactis-based plasmid manufacturing is the availability of high-copy number plasmids. Unfortunately, the highest copy number reported in Gram-positive bacteria for th...

Screening of commercial enzymes for transfructosylation of tyrosol: Effect of process conditions and reaction network.

Enzymatic fructosylation of organic acceptors other than saccharides brings new possibilities to synthesize molecules that do not exist in nature. The introduction of fructosyl moiety may lead to glycosides possessing enhanced physico-chemical and bioactive properties which could be useful in the pharmaceutical and cosmetic industry. In this work, the regioselective synthesis of tyrosol β-D-fructofuranoside catalyzed by β-fructofuranosidase is investigated. In the first step, thirty-two commercial enzyme ...

Enhanced Saccharification and Fermentation of Rice Straw by Reducing the Concentration of Phenolic Compounds Using an Immobilized Enzyme Cocktail.

The production of bioethanol from rice straw can contribute to the rural economy and provide clean fuel in a sustainable manner. However, phenolic compounds, which are mostly produced during acid pretreatment of biomass, act as inhibitors of fermenting microorganisms. Laccase is well known for its ability to oxidize lignin and phenolic compounds derived from lignocellulosic biomass. In the present study, an immobilized enzyme cocktail containing laccase was evaluated in regards to its ability to enhance the...

Production of 3-fucosyllactose in engineered Escherichia coli with α-1,3-fucosyltransferase from Helicobacter pylori.

3-Fucosyllactose (3-FL), one of the major oligosaccharides in human breast milk, was produced in engineered Escherichia coli. In order to search for a good α-1,3-fucosyltransferase, three bacterial α-1,3-fucosyltransferases were expressed in engineered E. coli deficient in β-galactosidase activity and expressing the essential enzymes for production of GDP-l-fucose, the donor of fucose for 3-FL biosynthesis. Among the three enzymes tested, the fucT gene from Helicobacter pylori NCTC 11637 gave the best 3-...

Newly Identified HNP-F from Human Neutrophil Peptide 1 Promotes Hemostasis.

Active hemostatic agents can play a crucial role in saving patients' lives during surgery. Active hemostats have several advantages including utilization of natural blood coagulation and biocompatibility. Among them, although HNP-1 was previously reported with the hemostatic mechanism, which part of HNP-1 was not known that facilitates the hemostatic activity. Here, we newly identified a partial peptide (HNP-F) promoting hemostasis originated from human neutrophil peptide 1 by blood coagulation ability test...

Efficient human genome editing using SaCas9 ribonucleoprotein complexes.

Genome editing using RNA-guided nucleases in their ribonucleoprotein (RNP) form represents a promising strategy for gene modification and therapy because they are free of exogenous DNA integration and have reduced toxicity in vivo and ex vivo. However, genome editing by Cas9 nuclease from Staphylococcus aureus (SaCas9), has not been reported in its RNP form, which recognizes a longer protospacer adjacent motif (PAM), 5'-NNGRRT-3', compared with SpCas9(Streptococcus pyogenes Cas9) of 5'-NGG-3' PAM. Here, we ...

In silico Metabolic Design of Two-strain Biofilm Systems Predicts Enhanced Biomass Production and Biochemical Synthesis.

Engineered biofilm consortia have the potential to solve important biotechnological problems that have proved difficult for monoculture biofilms and planktonic consortia such as conversion of lignocellulosic material to useful biochemicals. While considerable experimental progress has been reported for engineering and characterizing biofilm consortia, the field still lacks in silico tools for simulation, design and optimization of stable, robust and productive designed consortia. We developed biofilm consor...

Evolutionary engineering of Corynebacterium glutamicum.

A unique feature of biotechnology is that we can harness the power of evolution to improve process performance. Rational engineering of microbial strains has led to the establishment of a variety of successful bioprocesses, but is hampered by the overwhelming complexity of biological systems. Evolutionary engineering represents a straightforward approach for fitness-linked phenotypes (e.g. growth or stress tolerance) and has been successfully applied to select for strains with improved properties for the pa...

Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli.

The targeted increase of cellular ATP turnover (enforced ATP wasting) has recently been recognized as a promising tool for metabolic engineering when product synthesis is coupled with net ATP formation. The goal of the present study was to further examine and to further develop the concept of enforced ATP wasting and to broaden its scope for potential applications. In particular, considering the fermentation products synthesized by E. coli under anaerobic conditions as a proxy for target chemical(s), we (i)...

Next Generation Industrial Biotechnology-Transforming the Current Industrial Biotechnology into Competitive Processes.

Chemical industry has made contribution to modern society, providing cost competitive products for our daily usages. However, it now faces a serious challenge regarding environmental pollutions and greenhouse gas emission. With the rapid development of molecular biology, biochemistry and synthetic biology, industrial biotechnology has evolved to become more efficient for production of chemicals and materials. However, in contrast to chemical industries, current industrial biotechnology (CIB) is still not co...

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria.

Cyanobacteria are of great importance to Earth's ecology. Due to their capability in photosynthesis and C1 metabolism, they are ideal microbial chassis that can be engineered for direct conversion of carbon dioxide and solar energy to biofuels and biochemicals. Facilitated by the elucidation of basic biology of the photoautotrophic microbes and rapid advances in synthetic biology, genetic toolkits have been developed to enable implementation of non-natural functionalities in engineered cyanobacteria. Hence,...

Systems Metabolic Engineering Meets Machine Learning: A new era for data-driven metabolic engineering.

The recent increase in high-throughput capacity of 'omics datasets combined with advances and interest in machine learning have created great opportunities for systems metabolic engineering. In this regard, data-driven modeling methods have become increasingly valuable to metabolic strain design. In this review we discuss the nature of 'omics and provide a broad introduction to the machine learning algorithms combining these datasets into predictive models of metabolism and metabolic rewiring. Next, we high...

Biotechnology, Big Data and Artificial Intelligence.

Developments in biotechnology are increasingly dependent on the extensive use of big data, generated by modern high-throughput instrumentation technologies and stored in thousands of databases, public and private. Future developments in this area depend, critically, on the ability of biotechnology researchers to master the skills required to effectively integrate their own contributions with the large amounts of information available in these databases. This article offers a perspective of the relations tha...

Development of a RecE/T-assisted CRISPR-Cas9 toolbox for Lactobacillus.

Lactobacilli are members of a large family involved in industrial food fermentation, therapeutics, and health promotion. However, the development of genetic manipulation tools for this genus lags behind its relative industrial and medical significance. The development of CRISPR-based genome engineering for Lactobacillus is now underway. Generally, the CRISPR-Cas9 system relies on targeted Cas9/sgRNA complex-induced double-strand breaks (DSBs) to stimulate recombination repair, which allows chromosomal genet...

Coculture of Endothelial Cells with Human Pluripotent Stem Cell-Derived Cardiac Progenitors Reveals a Differentiation Stage-Specific Enhancement of Cardiomyocyte Maturation.

Cardiomyocytes (CMs) generated from human pluripotent stem cells (hPSCs) are immature in their structure and function, limiting their potential in disease modeling, drug screening, and cardiac cellular therapies. Prior studies have demonstrated that coculture of hPSC-derived CMs with other cardiac cell types, including endothelial cells (ECs), can accelerate CM maturation. To address whether the CM differentiation stage at which ECs are introduced affects CM maturation, we cocultured hPSC-derived ECs with h...

Experimental Evaluation of the Impact of Intrinsic Process Parameters on the Performance of a Continuous Chromatographic Polishing Unit (MCSGP).

The semi-continuous twin-column MCSGP process improves the trade-off between purity and yield encountered in traditional batch chromatography, while its complexity in terms of hardware requirements and process design is reduced in comparison to process variants using more columns. In this study, the MCSGP process was experimentally characterized specifically with respect to its unique degrees of freedom, i.e. the four switching times, which alternate the columns between interconnected and batch states. By m...

Pathway engineering of Bacillus subtilis for enhanced N-acetylneuraminic acid production via whole-cell biocatalysis.

N-acetylneuraminic acid (NeuAc) is a common sialic acid that has wide-ranging applications as nutraceuticals and pharmaceuticals. However, low production efficiency and high environmental pollution of traditional extraction and chemical synthesis methods constrain NeuAc supply. Here, we developed a biological approach for food-grade NeuAc production via whole-cell biocatalysis by generally regarded as safe (GRAS) bacterium Bacillus subtilis. Promoters for controlling N-acetylglucosamine 2-epimerase (AGE) an...

Engineering of phosphoserine aminotransferase increases the conversion of L-homoserine to 4-hydroxy-2-ketobutyrate in a glycerol-independent pathway of 1,3-propanediol production from glucose.

Phosphoserine aminotransferase (SerC) from Escherichia coli MG1655 was engineered to catalyze the deamination of homoserine to 4-hydroxy-2-ketobutyrate, a key reaction in producing 1,3-propanediol (1,3-PDO) from glucose in a novel glycerol-independent metabolic pathway. To this end, a computation-based rational approach was used to change the substrate specificity of SerC from L-phosphoserine to L-homoserine. In this approach, molecular dynamics simulations and virtual screening were combined to predict mut...

Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single Cell Level.

Biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells requires the generation of high-producing clonal cell lines. During cell line development, cell cloning using fluorescence activated cell sorting (FACS) has the potential to combine isolation of single cells with sorting based on specific cellular attributes that correlate with productivity and/or growth, identifying cell lines with desirable phenotypes for manufacturing. This study describes the application of imaging flow cytometry (I...

Oil palm biomass biorefinery for sustainable production of renewable materials.

Oil palm biomass is widely known for its potential as a renewable resource for various value-added products due to its lignocellulosic content and availability. Oil palm biomass biorefinery is a certain homerun money-making industry that comes with socio-political benefits through job opportunities created as well as a great environmental conservation initiative. Many studies have been conducted on the technological advancements of oil-palm biomass-derived renewable materials, which are discussed comprehens...

Yeast Systems Biology: Model organism and Cell Factory.

For thousands of years the yeast Saccharomyces cerevisiae has served as a cell factory for the production of bread, beer, and wine. In more recent years, this yeast has also served as a cell factory for producing many different fuels, chemicals, food ingredients and pharmaceuticals. S. cerevisiae, however, has also served as a very important model organism for studying eukaryal biology, and even today many new discoveries, important for the treatment of human diseases, are made using this yeast as a model o...

Systems metabolic engineering strategies for non-natural microbial polyester production.

Plastics we use everyday are mostly synthetic polymers derived from fossil resources, and their accumulation is becoming a serious concern world-wide. Polyhydroxyalkanoates (PHAs) are naturally produced polyesters synthesized and intracellularly accumulated by many different microorganisms. PHAs are a good alternative to petroleum based plastics since they possess a wide range of material properties depending on the monomer types and molecular weights. In addition, PHAs are biodegradable, and can be produce...

AFOB Summer Forum - A 'Davos Forum' for Asian Biotechnology.

Biomimetic and Bioinspired Biotechnology.

Emerging Technologies for Low-Cost, Rapid Vaccine Manufacture.

To stop the spread of future epidemics and meet infant vaccination demands in low- and middle-income countries, flexible, rapid and low-cost vaccine development and manufacturing technologies are required. Vaccine development platform technologies that can produce a wide range of vaccines are emerging, including: a) humanized, high-yield yeast recombinant protein vaccines; b) insect cell-baculovirus ADDomer vaccines; c) Generalized Modules for Membrane Antigens (GMMA) vaccines; d) RNA vaccines. Herein, exis...


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