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18:26 EDT 24th June 2019 | BioPortfolio

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Showing PubMed Articles 1–25 of 70 from Cryo letters

Hyaluronidase Pretreatment Improves the Cryopreservation of Human Ovarian Tissue.

Slow freezing has been widely applied to preserve human ovarian tissue.

MALDI-TOF MS Spectral Variation Is Observed between Fungal Samples Grown under Identical Conditions after Long-term Storage by Cryopreservation, Freeze-drying, and under Oil.

Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) offers a powerful, versatile, and relatively-inexpensive tool for the characterization and/or identification of protein-containing samples.

Effect of Melatonin on Cryopreserved Sperm of Prochilodus lineatus (Characiformes).

Semen cryopreservation is a technique widely used in commercial and conservation fish farms. However, the success of the technique will depend on the diluent and cryoprotectant solutions added to the semen prior to the freezing procedure.

Effect of EDTA as Chelating Agent in Extender on the Post Thaw Quality of Buffalo Bull Spermatozoa.

Spermatozoa are prone to mechanical and biochemical stresses upon freezing. The influx of Ca causes early capacitation and production of reactive oxygen species.

Numerical Study and Experimental Verification of Tissue Cryofreezing Based on Flexible Cryoprobe System.

Cryosurgery is effectively used for treating various diseases including cancer and tumor. But there is difficulty accurately quantifying the spatial temperature profile needed to determine diseased tissue destruction.

Effect of Cryopreservation on Casa Characteristics, Mitochondrial Transmembrane Potential, Plasma and Acrosome Integrities, Morphology and in vivo Fertility of Buffalo Bull Spermatozoa.

Application of frozen-thawed semen is an important tool for improving the vivo fertility, but the process of freezing and thawing causes significant damage to spermatozoa.

A Simple and Reliable Cooling Approach for the Cryopreservation of Hematopoietic Stem Cells: the Passive Cooling Rate-controlled Technique.

Cryopreservation of hematopoietic stem cells (HSCs) has widely been used in stem cell transplantation and cellular therapy treating various human diseases. However, the current conventional cooling approach for the cryopreservation of HSCs has the following potential problems: (1) requirement of a very expensive computer-programmed liquid nitrogen freezer (LNF) for the cooling rate control, (2) a large consumption of liquid nitrogen, (3) periodic breakdown of the LNF due to the mechanical failure of the liq...

Human Gametes Cryopreservation with Cryoprotectant Modified by Egg Yolk.

The modification of cryoprotectants with an egg yolk is an effective method to improve cell survival.

Impact of Distinct Freezing Curves on the Quality of Ram Sperm after Thawing.

Automated equipment with customized freezing curves can be used to cryopreserve ram sperm, but none is considered a standard.

Application of Pectin from Aloe arborescens Mill. to the Cryopreservation of Human Leukocyte Cell Suspensions.

The molecular weight (MW) of pectic polysaccharide АА3 equal to 150 kDa and its structure suggest that it has a non-permeating protector action.

Effect of L-Tryptophan on Sperm Quality of Tigris Scraper (Capoeta umbla) (Pisces: Cyprinidae) after Cryopreservation.

The aromatic amino acid L-tryptophan is a versatile molecule and needed for the biosynthesis of proteins. Due to metabolic functions, it has been widely used in research and clinical trials.

The Impact of Cryopreservation-Thawing Conditions on Umbilical Cord Blood Quality and Transplantation Outcomes.

Cord blood units (CBUs) go through the cryopreservation-thawing process for storage before use in transplantation. The differences in the cryopreservation-thawing process affect the quality of CBUs. The effects of the cryopreservation-thawing process on the final outcomes of CBU transplantation has not been defined well.

Effect of Tris-egg Yolk, Soya Milk, and Liposome-based Extenders on Sahiwal (Bos indicus) Sperm Quality during Pre- and Post-Cryopreservation Stages.

Even though there are plenty of semen cryopreservation extenders available, their adoption is limited. Although normal tris-based egg yolk (EYC) extender is widely used, it leads to compromised post-thaw sperm quality.

Modifications to a Vitis Shoot Tip Cryopreservation Procedure: Effect of Shoot Tip Size and Use of Cryoplates.

Cryopreservation has been considered a preferred method for the long-term storage of plant germplasm, especially to efficiently conserve and maintain the genetic integrity of genebank materials. Droplet-vitrification (DV) procedures have been developed to cryopreserve Vitis shoot tips from in vitro-grown plants.

Cryopreservation of Endangered Wild Species, Aster altaicus var. uchiyamae Kitam, Using Droplet-Vitrification Procedure.

Aster altaicus var. uchiyamae Kitam is an endemic and endangered species in urgent need of a comprehensive conservation strategy.

Using Rapid I Method for Vitrification of Bovine Oocytes.

Vitrification is commonly used for cryopreservation of gametes.

Optimization of the Protocol for Cryopreservation of Arabian Stallion Spermatozoa: Effects of Centrifugation, Semen Extenders and Cryoprotectants.

Cryopreservation of Arabian stallion semen is important in order to improve the function and fertility of frozen/thawed semen in this breed.

Safer Vitrification of Mouse and Human Embryos Using the Novel Cryoroom Vitrification System for Assisted Reproductive Technology.

Vitrification is widely used for assisted reproductive technology (ART). Most vitrification devices require the skillful placement of embryos into the carrier and aspiration of excessive vitrification solution.

Effect of Different Cryoprotectants (Glycerol, Methanol and Dimethyl Sulfoxide) on Post-thaw Quality, Viability, Fertilization Ability and DNA Damage of Cryopreserved Nile Tilapia (Oreochromis niloticus) Spermatozoa.

Cryopreservation of sperm from different fish species requires different protocols. Therefore, it is necessary to perform studies to establish reliable procedures for each species.

Development of Cryopreservation Protocol for Aquilaria malaccensis Lam., a Recalcitrant Seeded Tropical Tree Species.

Cryopreservation is the only possible process for long-term germplasm storage of species with recalcitrant behavior. Aquilaria malaccensis is a recalcitrant seeded tropical tree that produces a distinctive fragrance which has high value in the commercial market.

Cryopreservation of Encapsulated Axillary Buds of Clitoria ternatea (L.).

Clitoria ternatea is a brain revitalizing legume, with immense pharmacological value including antimicrobial, antioxidant and anticancer. The lack of a commercial cultivation has led to its' over collection from the wild to meet the demand of herbal pharmaceutical sector and the species is now rare in the wild. Hence, the plant needs to be conserved. Cryopreservation of the species would supplement the conventional conservation strategies in the field or seed bank.

Seed Cryostorage Enhances Subsequent Plant Productivity in the Forage Species Teramnus Labialis (L.F.) Spreng.

Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryostorage has been shown to be beneficial for both seed storage and breaking physical dormancy in seeds of various species. However, its potential effects on subsequent seedling...

Simulation of Hypothermic Perfusion of Rabbit Livers for Cryopreservation Applications.

In cryopreservation of biological material, it is very important to precise control the perfusion of cryoprotectants (CPA) inside the sample. CPA concentration inside tissues and organs during the process was measured in a few studies. The simulation of the CPA perfusion inside the organ is still necessary to understand and optimize this complex process.

Isochoric Containers and Its Frontier between Cryopreservation and Sterilization.

The use of isochoric containers below the freezing point was proposed for the reduction of freezing damage.

Texture Feature Differences between Fresh and Frozen-thawed Ex-vivo Porcine Liver Tissue in B-mode Ultrasonic Imaging.

Ultrasound guidance of cryotherapy could be improved if changes of tissue characterization parameters during freezing could be used to monitor freezing profiles and estimate frostbite distributions.

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