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PubMed Journal Database | Electrophoresis RSS

17:42 EDT 24th June 2019 | BioPortfolio

The US National Library of Medicine and National Institutes of Health manage PubMed.gov which comprises of more than 29 million records, papers, reports for biomedical literature, including MEDLINE, life science and medical journals, articles, reviews, reports and  books.

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For example view all recent relevant publications on Epigenetics and associated publications and clincial trials.

Showing PubMed Articles 1–25 of 409 from Electrophoresis

Direct observation of single plasmonic metal nanoparticle reaction in microcolumn with chromatic-aberration-free LASER light-sheet scattering imaging.

Direct observation and characterization of individual noble metal nanoparticles (MNPs) and their chemical reactions has attracted much attentions owing to their unique physical and chemical properties and extensive applications. To achieve high-throughput information-rich evaluation of MNPs, it would be advantageous to combine highly efficient microcolumn separation technology with on-column high resolution plasmonic imaging technique. Here, with a chromatic aberration-suppressed supercontinuum laser light-...

MS-based strategies for identification of protein SUMOylation modification.

Protein SUMOylation modification conjugated with small ubiquitin-like modifiers (SUMOs) is one kind of post-translational modifications (PTMs), which exerts comprehensive roles in cellular functions, including gene expression regulation, DNA repair, intracellular transport, stress responses and tumorigenesis. With the development of the peptide enrichment approaches and mass spectrometry (MS) technology, more than 6000 SUMOylated proteins and about 40000 SUMO acceptor sites have been identified. In this rev...

Image-based Sorting and Negative Dielectrophoresis for High Purity Cell and Particle Separation.

Microelectrode arrays are used to sort single fluorescently labelled cells and particles as they flow through a microfluidic channel using dielectrophoresis (DEP). Negative DEP is used to create a "Dielectrophoretic Virtual Channel" (DVC) that runs along the centre of the microfluidic channel. By switching the polarity of the electrodes, the virtual channel can be dynamically reconfigured to direct particles along a different path. This is demonstrated by sorting particles into two microfluidic outlets, con...

Mathematical Model to Reduce Loop Mediated Isothermal Amplification (LAMP) false-positive diagnosis.

Loop mediated isothermal amplification (LAMP) is a nucleic acid amplification technique performed under isothermal conditions. The output of this amplification technique includes multiple different sizes of DNA structures which are identified by a banding pattern on gel electrophoresis plots. Although this is a specific amplification technique, the complexity of the primer design and amplification still lead to the issue of obtaining false-positive results, especially when a positive reading is determined s...

Design and optimization of a fused-silica microfluidic device for separation of trivalent lanthanides by isotachophoresis.

Elemental analysis of rare earth elements is essential in a variety of fields including environmental monitoring and nuclear safeguards; however, current techniques are often labor intensive, time consuming and/or costly to perform. The difficulty arises in preparing samples, which requires separating the chemically and physically similar lanthanides. However, by transitioning these separations to the microscale, the speed, cost, and simplicity of sample preparation can be drastically improved. Here, all fo...

The effects of electrostatic correlations on the ionic current rectification in conical nanopores.

Ion-ion electrostatic correlations are recognized to play a significant role in the presence of concentrated multivalent electrolytes. To account for their impact on ionic current rectification phenomenon in conical nanopores, we use the modified continuum Poisson-Nernst-Planck (PNP) equations by Bazant et al. Coupled with the Stokes equations, the effects of the EOF are also included. We thoroughly investigate the dependence of the ionic current rectification ratios as a function of the double layer thickn...

A new droplet-breakup phenomenon in electrokinetic flow through a microchannel constriction.

A completely new droplet breakup phenomenon is reported for droplets passing through a constriction in an electrokinetic flow. The breakup occurs during the droplet shape recovery process past the constriction throat by the interplay of the dielectrophoretic (DEP) stress release and the interface energy for droplets with smaller permittivity than that of the ambient fluid. There are conditions for constriction ratios an droplet size that the droplet breakup occurs. The numerical predictions provided here re...

Design of experiments as a valuable tool for biopharmaceutical analysis with (imaged) capillary isoelectric focusing.

Capillary isoelectric focusing is indispensable for characterizing charge heterogeneity and isoelectric points of biopharmaceuticals. However, there are many influencing parameters and therefore method development is challenging. This study was performed to obtain an in-depth understanding of the imaged CIEF methodology by applying a Design of Experiments approach. To describe the parameter's effects as objectively as possible, a polynomial regression model was derived for the most important responses. For ...

Continuous flow microfluidic cell inactivation with use of insulating micropillars for multiple electroporation zones.

Electroporation is a powerful tool for inactivating cells and transfecting biological cells and has applications in biology, genetic engineering, medicine, environment, and many others. We report a new continuous flow device embedded with insulating micropillars to achieve better performance of cell inactivation. The use of micropillars creates multiple electroporation zones with enhanced local electric field strengths. Using a model solution of Saccharomyces cerevisiae, we examined the inactivation perform...

Rapid detection of trace Cu using an L-cysteine based interdigitated electrode sensor integrated with AC electrokinetic enrichment.

Copper is an indispensable trace element for human health. Too much or too little intake of copper ion (Cu ) can lead to its own adverse health conditions. Therefore, detection of Cu is always of vital importance. In this work, a simple sensor was developed for rapid detection of trace Cu in water, in which L-cysteine (Cys) as a molecular probe was self-assembled on a gold interdigital electrode (IDE) to form a monolayer for specific capture of Cu . The interfacial capacitance of the IDE was detected to ind...

A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China.

A novel analytical protocol was developed for general quality screening of chicken meat based on isoelectric focusing (IEF) and protein extraction. To demonstrate the developed protocol, twenty-four chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water and Group 3 were infected by new castle disease virus (NDV), respectively. Proteins were extracted from the meat samples by using pure water as an extractant,...

Comparison of the peptidome released from keratins in Saiga antelope horn and goat horn under simulated gastrointestinal digestion.

Goat horn (Caprae Hircus Cornu, GH) has been used as a substitute for Saiga antelope horn (Saigae Tataricae Cornu, SAH) in the clinic and the pharmaceutical industry. In the present study, peptides released from SAH and GH under simulated gastric and intestinal digestion were identified. The results showed that most of the peptides released from SAH and GH under simulated gastrointestinal digestion were hydrophilic, and over 75% of the peptides from keratins (KRTs) were hydrophilic. In total, over 58% of th...

Capillary electrophoresis/visible-LED induced fluorescence of tryptophan: What's new?

Tryptophane (Trp) labelled by 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) is very difficult to identify using capillary electrophoresis and fluorescence detection (480 nm). Why? In this article some mass spectrometry experiments show that Trp is really labelled by CBQCA as Leucine (Leu). If the maximum of UV absorption (λ ) is the same between Leu-CBQCA and Trp-CBQCA, the molar extinction coefficient is around 2 fold higher for Trp-CBQCA. The fluorescence of the Leu-CBQCA derivative is 50 more i...

Chiral separation of Cathinone derivatives using β-cyclodextrin-assisted capillary electrophoresis - Comparison of four different β-cyclodextrin derivatives used as chiral selectors.

In the past decade, more than 100 different cathinone derivatives slopped over entire Europe due to their meanwhile enormous popularity. Generally, these Novel Psychoactive Substances are easy available via the internet. This fact leads to various social problems, since cathinones are substances with consciousness-changing effects and are mainly misused for recreational matters by their consumers. Cathinones possess a chiral centre including two enantiomeric forms with potentially different pharmacological ...

Comparative proteomics analysis between biofilm and planktonic cells of Mycobacterium tuberculosis.

Tuberculosis (TB) is highly persistent and displaying phenotypic resistance to high concentrations of antimicrobials. Recent reports exhibited that Mycobacterium tuberculosis (M. tuberculosis) biofilm was implicated to its pathogenicity and drug resistance. In this study, there were 47 kinds of differential proteins in the biofilm of M. tuberculosis H37Rv cells compared with the planktonic bacteria, and 37 proteins were non-redundant identified by proteomics approach, such as two-dimensional gel electrophor...

CE-SDS method development, validation and best practice - an overview.

CE-SDS has been implemented in the biopharmaceutical industry and is being used for the characterization of therapeutic proteins in most Biological License Applications currently submitted. An overview is presented on the separation mechanism, methodology and good working practices / best practices. The CE-SDS platform method development and validation are discussed and typical scientifically and regulatory issues and troubleshooting situations are highlighted. This article is protected by copyright. All ri...

Study of the effect of culture-mediums on the amino acid metabolites for corynebacterium glutamicum using high-speed micellar electrokinetic chromatography.

Corynebacterium glutamicum (C. glutamicum) is a well known workhorse for the industrial production of amino acids. Different carbon, nitrogen and sulphur source may force the bacterium produce specific metabolites. In this work, a method of high-speed MEKC with LIF detection was developed to rapid analyze the amino acid metabolites released by C. glutamicum fed with different culture-mediums. C. glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. ...

Determination of chlorine species by capillary electrophoresis - mass spectrometry.

The applicability of capillary zone electrophoresis with mass spectrometric detection for the determination of four chlorine species, namely chloride and three stable chlorine oxyanions was studied. The main aspects of the proper selection of background electrolyte and sheath liquid for the CE-MS determinations of anions with high mobility were demonstrated, pointing out the importance of pH and the mobility of the anion in the background electrolyte. The possibility of using uncoated fused silica capillary...

Graphene quantum dots for enhancement of fluorimetric detection coupled to capillary electrophoresis for detection of ofloxacin.

A new CE method with fluorescence detection is reported on the determination of ofloxacin in milk samples using graphene quantum dots (GQDs) for sensitivity enhancement. Injection of GQDs prior the standards/sample is crucial to increase the antibiotic fluorescence response. Clean-up and preconcentration steps allowed for a good linear correlation in a concentration range between 50 and 1000 ng/mL for the ofloxacin, detection and quantification limits being 10.7 ng/mL and 35.5 ng/mL, respectively. Optimal C...

A fresh look into background electrolyte selection for CE-LIF of peptides and proteins.

This study reports a reinvestigation of background electrolyte selection strategy for performance improvement in CE-LIF of peptides and proteins. This strategy is based on the employment of high concentrations of organic species in BGE possessing high buffer capacity and low specific conductivity in order to ensure excellent stacking preconcentration and separation resolution of fluorescently tagged peptides and proteins. Unlike universal UV detection, the use of such BGEs at high concentrations does not le...

On-chip technology for single-cell arraying, electrorotation-based analysis and selective release.

This paper reports a method for label-free single-cell biophysical analysis of multiple cells trapped in suspension by electrokinetic forces. Tri-dimensional pillar electrodes arranged along the width of a microfluidic chamber define actuators for single cell trapping and selective release by electrokinetic force. Moreover, a rotation can be induced on the cell in combination with a negative DEP force to retain the cell against the flow. The measurement of the rotation speed of the cell as a function of the...

Rapid electrophoretic recovery of DNA from dried blood spots.

Large-scale genetic screening of neonatal dried blood spots for episomal DNA has a great potential to lower patient mortality and morbidity through early diagnosis of primary immunodeficiencies. However, DNA extraction from the surface of dried blood spots remains one of the most time consuming, costly, and labor-intensive parts of DNA analysis. In the present study, we developed and optimized a rapid methodology using only 50V and heat to extract episomal DNA from dried blood spots prepared from diagnostic...

Recent development in chitosan nanocomposites for surface-based biosensor applications.

Recent years have witnessed ever expanding use of biosensors in the fields of environmental monitoring, homeland security, pharmaceutical, food and bioprocessing, and agricultural industries. To produce effective and reliable biosensors, good quality immobilization of biological recognition elements is critical. Chitosan and its nanocomposites emerge as an excellent immobilization matrix on biosensor surface. As a natural polysaccharide, chitosan has many useful characteristics, such as high permeability an...

Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosis.

Osteoporosis is an emerging health issue worldwide. Due to the decrease of bone mineral density and the deterioration of skeletal microarchitecture, osteoporosis could lead to increased bone fragility and higher fracture risk. Since lack of specific symptoms, novel serum proteomic indicators are urgently needed for the evaluation of osteoporosis. Microvesicles (MVs) are important messengers widely present in body fluids and have emerged as novel targets for the diagnosis of multiple diseases. In this study,...

Some thoughts about enantioseparations in capillary electrophoresis.

In this overview the goal of the authors was to analyze from the historical perspective the reasons of success and failure of chiral capillary electrophoresis. In addition, the current trends are analyzed, unique advantages of capillary electrophoresis are highlighted and some future directions are discussed. This article is protected by copyright. All rights reserved.


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