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Present study is designed to investigate the expression behavior of serum proteins that may serve as predictive and diagnostic biomarkers for gestational diabetes mellitus (GDM). This may also clarify the mechanisms associated with the origin and development of GDM.
Investigations of the molecular mechanisms underlying the metastatic phenotype of papillary thyroid cancer and identification of novel candidate biomarkers to better predict papillary thyroid cancer metastasis are urgently required.
Urine is an important resource for biomarker research. Urine proteins reflect not only renal diseases but also changes in other organs in the body. However, urine has rarely been used to reflect inflammatory bowel disease.
As an important part of the data-independent acquisition-mass spectrometry (DIA-MS) analysis approach, spectral library generation is closely related to the final protein identification and quantitation.
The tyrosine kinase inhibitor (TKI) sunitinib is a multi-targeted agent approved across multiple cancer indications. Nevertheless, since approval, data has emerged to describe a worrisome side effect profile including hypertension, hand-foot syndrome, fatigue, diarrhoea, mucositis, proteinuria and (rarely) congestive heart failure. It has been hypothesised that the observed multi-parameter toxicity profile is related to 'on-target' kinase inhibition in 'off-target' tissues. Herein, to interrogate off-target...
Identifying autoantigens of serological autoantibodies requires expensive methods, such as protein microarrays or immunoprecipitation plus mass spectrometry. Thus, sera are commonly pre-screened for interesting immunopatterns via immunocytochemistry/immunohistochemistry. However, distinguishing immunopatterns can be difficult and intracellular antigens are less accessible. Therefore, we present an alternative: a simple and cheap immunoblot screening able to detect proteins refractory to solubilization.
Kidney disease is one of the fastest growing causes of death worldwide, disclosing an unmet clinical need for early diagnosis and optimized risk stratification that allows high risk patient selection for clinical trials and for more intensive nephroprotective interventions in the clinic. The current issue of PROTEOMICS - Clinical Applications contains four manuscripts that explore different aspects of clinical proteomics implementation in the context of acute kidney injury, chronic kidney disease and, more ...
Acute promyelocytic leukemia (APL) is the most prognostically favorable subtype of AML. Defining the features that allow identification of acute promyelocytic leukemia (APL) patients likely to relapse after therapy remains challenging.
Using a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method, we have spatially profiled N-linked glycans in human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA). Routinely, 50 or more individual N-glycan species are detected per FFPE tissue, and this number increases significantly with disease severity. Through the combined analysis of breast cancer FFPE tissues and TMAs we have been able to identify ...
In biomedical research, mass spectrometry imaging (MSI) can generate molecular information in a spatially resolved manner from tissue sections. Especially matrix-assisted laser desorption/ionization (MALDI) MSI offers, depending on the type of matrix, the detection of a broad variety of molecules ranging from metabolites to proteins, thereby facilitating the collection of multi-level molecular data. Lately, integrative clustering techniques have been developed that make use of the complementary information ...
The current clinical investigation for primary aldosteronism (PA) diagnosis requires complex expensive tests from the initial suspicion to the final subtype classification, including invasive approaches; therefore, appropriate markers for subtype definition are greatly desirable. The present study performed a metabolomics analysis to further examine specific molecular signatures of PA urines EXPERIMENTAL DESIGN: : The study considered PA subtype and gender-related differences using two orthogonal advanced U...
Timely and accurate triaging of patients with community-acquired pneumonia (CAP) is difficult for clinicians. Pentraxin-3 (PTX3) is a non-specific marker of disease severity; however, PTX3 data from CAP patients are lacking.
Profiling histone post-translational modifications (PTMs) in clinical samples holds great potential for the identification of epigenetic biomarkers and the discovery of novel epigenetic targets. Mass spectrometry (MS)-based approaches to analyze histone PTMs in clinical samples usually rely on SDS-PAGE separation following histone enrichment in order to eliminate detergents and further isolate histones. However, this limits the digestions options and hence the modification coverage.
Precise histological classification of epithelial ovarian cancer (EOC) has immanent diagnostic and therapeutic consequences, but remains challenging in histological routine. The aim of this pilot study was to examine the potential of MALDI-Imaging mass spectrometry in combination with machine learning methods to classify EOC histological subtypes from tissue microarray.
To present matrix-assisted laser desorption/ionization (MALDI) imaging as a powerful method to highlight various tissue compartments.
Ureteropelvic junction obstruction (UPJO) is a common obstructive disease. To investigate the usefulness urinary biomarkers in UPJO children, we analyzed the urinary proteome in UPJO infants and compared it with normal controls.
To facilitate the transition of MALDI-MS Imaging (MALDI-MSI) from basic science to clinical application, it is necessary to analyze formalin-fixed paraffin-embedded (FFPE) tissues. Our aim was to improve in-situ tryptic digestion for MALDI-MSI of FFPE samples and determine if similar results would be reproducible if obtained from different sites.
Protein glycosylation, particularly N-linked glycosylation, is a complex post-translational modification (PTM), which plays an important role in protein folding and conformation, regulating protein stability and activity, cell-cell interaction, and cell signalling pathways. This review focuses on analytical techniques, primarily mass spectrometry-based techniques, to qualitatively and quantitatively assess N-glycosylation while successfully characterising compositional, structural and linkage features with ...
We aim to discover and identify early grade diagnosis markers in tumor microenvironment and investigated their expression in serum.
Actinic keratoses (AK) are premalignant tumors that can be clinically difficult to differentiate from skin cancer. An easy, quick and reliable non-invasive alternative to biopsy is needed to definitively confirm the clinical diagnoses. This study evaluates Tape Stripping (TS) of stratum corneum (SC) for non-invasive biomarker analysis of AK.
Rheumatoid arthritis (RA) is an autoimmune disease in which autoantibodies attack the synovial membrane, causing joint inflammation. Blood tests would offer a powerful, minimally invasive method for early diagnosis of RA. However, no reliable biomarkers for RA are presently available. Our aim was to develop biomarkers for RA by multiple reaction monitoring (MRM)-based quantification of candidate biomarkers.
In matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) standardized sample preparation is important to obtain reliable results. Herein, we investigated the impact of section thickness in formalin-fixed paraffin embedded (FFPE) tissue microarrays (TMA) on spectral intensities.
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI MSI) is a powerful tool to analyze the spatial distribution of peptides in tissues. Digital polymerase chain reaction (dPCR) is a method to reliably detect genetic mutations. Biopsy material is often limited due to minimally invasive techniques, but information on diagnosis, prognosis and prediction is required for subsequent clinical decision making. Thus, saving tissue material during diagnostic workup is highly wa...
Dialysis as renal replacement therapy, aims to remove excess water and waste solutes from the uremic patient while retaining proteins in the plasma. Irrespective of the dialysis modality, hemodialysis (HD) or peritoneal dialysis (PD), the amount and composition of proteins that are removed are important determinants of the biocompatibility of the therapy. Although hemodialysis membranes would ideally be biologically inert filtration tubes, they are known to adsorb proteins. The part of the plasma proteome t...
Approximately 5% of giant cell tumours of bone (GCT) develop pulmonary metastases. Although many biomarkers have been proposed, identification of circulating low abundance molecules may be useful to predict malignant progression.