PubMed Journals Articles About "Affimer Reagents Tools Diagnosing Plant Virus Diseases" RSS

12:22 EDT 20th June 2019 | BioPortfolio

Affimer Reagents Tools Diagnosing Plant Virus Diseases PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Affimer Reagents Tools Diagnosing Plant Virus Diseases articles that have been published worldwide.

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Showing "Affimer reagents tools diagnosing plant virus diseases" PubMed Articles 1–25 of 22,000+

Plant Virus Epidemiology: Applications and Prospects for Mathematical Modeling and Analysis to Improve Understanding and Disease Control.

In recent years, mathematical modeling has increasingly been used to complement experimental and observational studies of biological phenomena across different levels of organization. In this article, we consider the contribution of mathematical models developed using a wide range of techniques and uses to the study of plant virus disease epidemics. Our emphasis is on the extent to which models have contributed to answering biological questions and indeed raised questions related to the epidemiology and eco...

Harnessed viruses in the age of metagenomics and synthetic biology: An update on infectious clone assembly and biotechnologies of plant viruses.

Recent metagenomic studies have provided an unprecedented wealth of data, which is revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses as active players in the phytobiome, and surveys have uncovered their positive roles in environmental stress tolerance of plants. Viral infectious clones are key tools for functional characterization of known and newly identified viruses. Knowledge of viruses and their components has been instrumental for the development of modern pla...

A new strategy of using satellite RNA to control viral plant diseases: Post-inoculation with satellite RNA attenuates symptoms derived from pre-infection with its helper virus.

Satellite RNA (satRNA) is the supernumerary RNA of some viruses. It typically shares no nucleotide homology with its helper virus and depends on its helper virus for replication and encapsidation (Simon et al. 2004). The presence of satRNA sometimes interferes with the replication and symptom expression of its helper virus. Satellite RNA can have neutral, attenuated, or intensified effects on the virulence of its helper virus owing to complicated interactions among satRNA variants, helper viruses, and host...

Plant Virus Vectors 3.0: Transitioning into Synthetic Genomics.

Plant viruses were first implemented as heterologous gene expression vectors more than three decades ago. Since then, the methodology for their use has varied, but we propose it was the merging of technologies with virology tools, which occurred in three defined steps discussed here, that has driven viral vector applications to date. The first being the advent of molecular biology and reverse genetics, which enabled the cloning and manipulation of viral genomes to express genes of interest (vectors 1.0). Th...

Providence virus: An animal virus that replicates in plants or a plant virus that infects and replicates in animal cells?

Emerging viral diseases, most of which are zoonotic, pose a significant threat to global health. There is a critical need to identify potential new viral pathogens and the challenge is to identify the reservoirs from which these viruses might emerge. Deep sequencing of invertebrate transcriptomes has revealed a plethora of viruses, many of which represent novel lineages representing both plant and animal viruses and little is known about the potential threat that these viruses pose.

Forecasting the West-Nile virus in the USA: a Google Trends-based time series analysis.

West-Nile virus (WNV) is an arbovirus responsible of an infection, which tends to peak during the late summer and early fall period. Recently, tools monitoring web searches are emerging as powerful sources of data concerning especially infectious diseases.

Plant-GQ: An Integrative Database of G-Quadruplex in Plant.

G-quadruplex (G-Q) is advanced DNA or RNA secondary structures frequently found in plant and involved in important biological processes such as transcription, translation, and telomere maintenance. Although some databases and tools were developed for predicting and studying G-Q, none of them was for plant. With the development of next-generation sequencing technology, a large number of plant genomes have been assembled and annotated to provide opportunities for mining G-Q. Plant G-quadruplex database (Plant...

The cell tube block technique and an immunohistochemistry panel including Wilms tumor 1 to assist in diagnosing cavitary effusions in dogs and cats.

Cell blocks and immunohistochemistry (IHC) are increasingly recognized as being complementary tools for cytologic diagnostics, especially for neoplastic diseases.

Nanopore-based detection and characterization of yam viruses.

We here assessed the capability of the MinION sequencing approach to detect and characterize viruses infecting a water yam plant. This sequencing platform consistently revealed the presence of several plant virus species, including Dioscorea bacilliform virus, Yam mild mosaic virus and Yam chlorotic necrosis virus. A potentially novel ampelovirus was also detected by a complimentary Illumina sequencing approach. The full-length genome sequence of yam chlorotic necrosis virus was determined using Sanger sequ...

Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR).

The Virus Pathogen Resource (ViPR; ) is a US National Institute of Allergy and Infectious Diseases (NIAID)-sponsored Bioinformatics Resource Center providing bioinformatics support for major human viral pathogens. The hepatitis C virus (HCV) portal of ViPR facilitates basic research and development of diagnostics and therapeutics for HCV, by providing a comprehensive collection of HCV-related data integrated from various sources, a growing suite of analysis and visualization tools for data m...

Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System.

Targeted modification of plant genomes is a powerful strategy for investigating and engineering cellular systems, paving the way for the discovery and development of important, novel agricultural traits. Cas9, an RNA-guided DNA endonuclease from the type II adaptive immune CRISPR system of the prokaryote Streptococcus pyogenes, has gained widespread popularity as a genome-editing tool for use in a wide array of cells and organisms, including model and crop plants. Effective genome engineering requires the d...

Ebola virus disease-related stigma among survivors declined in Liberia over an 18-month, post-outbreak period: An observational cohort study.

While qualitative assessments of Ebola virus disease (EVD)-related stigma have been undertaken among survivors and the general public, quantitative tools and assessment targeting survivors have been lacking.

Multiplexed FluoroSpot for the Analysis of Dengue Virus- and Zika Virus-Specific and Cross-Reactive Memory B Cells.

Dengue virus (DENV) and Zika virus (ZIKV) are mosquito-borne pathogens that have a significant impact on human health. Immune sera, mAbs, and memory B cells (MBCs) isolated from patients infected with one DENV type can be cross-reactive with the other three DENV serotypes and even more distantly related flaviviruses such as ZIKV. Conventional ELISPOTs effectively measure Ab-secreting B cells but because they are limited to the assessment of a single Ag at a time, it is challenging to distinguish serotype-sp...

Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China.

Mammarenaviruses are associated with human hemorrhagic fever diseases in Africa and America. Recently, a rodent mammarenavirus, Wēnzhōu virus (WENV) and related viruses, have been reported in China, Cambodia, and Thailand. Moreover, in Cambodia, these viruses were suspected to be associated with human disease. In China, Yunnan Province is famous for its abundant animal and plant diversity and is adjacent to several South-eastern Asia countries. Therefore, it is necessary to know whether WENV-related virus...

Development of a cucumber green mottle mosaic virus-based expression vector for the production in cucumber of neutralizing epitopes against a devastating animal virus.

Virus-based expression systems have been widely exploited for the production of recombinant proteins in plants during the last thirty years. Advances in technology have boosted scale-up manufacturing of plant-made pharmaceuticals to high levels, via the complementation of transient expression and viral vectors. This combination allows proteins of interest to be produced in plants within a matter of days and thus, is well suited for the development of plant-made vaccines or therapeutics against emerging infe...

A spatial association between a Nipah virus outbreak in Kozhikode, Kerala, India and Nipah virus infection in Pteropus bats.

Detecting vertical Zika transmission: emerging diagnostic approaches for an emerged flavivirus.

Zika virus was recently responsible for a massive epidemic that spread throughout Latin America and beyond. Though typically asymptomatic or self-limiting, the sheer numbers of Zika infections allowed identification of unexpected phenotypes including sexual transmission, Guillain-Barré syndrome, and teratogenicity. Thousands of infants in South, Central, and North America have now been born with microcephaly or one of a number of fetal anomalies constituting the congenital Zika syndrome (CZS). Diagnosing C...

Endosomal escape and cytosolic penetration of macromolecules mediated by synthetic delivery agents.

Cell delivery reagents often exploit the endocytic pathway as a route of cell entry. Once endocytosed, these reagents must overcome endosomal entrapment to insure the release of their macromolecular cargos into the cytosol of cells. In this review, we describe several examples of prototypical synthetic reagents that are capable of endosomal escape and examine their mechanisms of action, their efficiencies, and effects on cells. Although these delivery systems are chemically distinct, some commonalities in h...

Anthropogenic influences on emergence of vector-borne plant viruses: the persistent problem of Potato virus Y.

Potato virus Y (PVY) has reemerged as a serious impediment to seed potato production, responsible for reduced yields and tuber quality, as well as the majority of seed lot rejections by certification programs due to excessive virus incidence. This has led to seed shortages, especially in cultivars highly susceptible to infection. While seed certification programs have been effective at managing many virus diseases below economic thresholds, PVY has rapidly evolved in recent decades to become a complex of st...

Plant health emergencies demand open science: Tackling a cereal killer on the run.

Outbreaks of emerging plant diseases and insect pests are increasing at an alarming rate threatening the food security needs of a booming world population. The role of plant pathologists in addressing these threats to plant health is critical. Here, we share our personal experience with the appearance in Bangladesh of a destructive new fungal disease called wheat blast and stress the importance of open-science platforms and crowdsourced community responses in tackling emerging plant diseases. Benefits of th...

Real time detection and quantification of Epstein Barr virus in different grades of oral gingivitis and periodontitis patients.

Periodontal diseases are of microbial etiology and are globally causing loss of teeth in adult population. Many severe oral diseases have been recently associated to Herpes viruses, of which Epstein Barr Virus (EBV) and human cytomegalovirus (HCMV) have been indicated in the etiology of periodontal diseases.

Integrating Local Lesion Assays with Conventional RT-PCR for Detection of Interspecies Tospovirus Reassortants and Mixed Tospovirus Infections.

Tomato spotted wilt virus (TSWV) has historically been the major tospovirus present in North America. Recent emergence of Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) in Florida and the Caribbean has complicated reliable identification of tospoviruses in this region. Field symptoms of these three tospoviruses are indistinguishable in most host plants, and commercially available TSWV lateral-flow immunoassay reagents cross react with GRSV and TCSV, leading to incorrect diagnoses of ...

High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy.

This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were ...

The significance of methionine cycle enzymes in plant virus infections.

Both biotic and abiotic stresses cause changes in the activities of plant methionine cycle (MTC) enzymes. These changes contribute to the ability of the plant to manage stress. On the other hand, viruses utilize MTC enzymes to promote infection. Here, we review the growing but still limited knowledge of the interactions between plant viral proteins and MTC enzymes. Virus-induced changes in S-adenosyl methionine synthetase and S-adenosyl homocysteine hydrolase activities debilitate transcriptional and post-t...

Zika Virus Diagnosis: Challenges and Solutions.

Since its sudden appearance and link to microcephaly in 2015, the number of PubMed references for Zika virus (ZIKV) has risen from 181 to 5,163, as of writing, with a vast proportion focused on the consequences of ZIKV infection during pregnancy. This level of attention underlies increased demand for sensitive and specific diagnostic tools able to assess risk to an unborn child, as well as to understand the dynamics and consequences of viral persistence.

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