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PubMed Journals Articles About "Antibiotics Versus Biofilm Emerging Battleground Microbial Communities" RSS

19:55 EDT 20th June 2019 | BioPortfolio

Antibiotics Versus Biofilm Emerging Battleground Microbial Communities PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Antibiotics Versus Biofilm Emerging Battleground Microbial Communities articles that have been published worldwide.

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Showing "Antibiotics versus biofilm emerging battleground microbial communities" PubMed Articles 1–25 of 11,000+

Rhamnolipid-involved antibiotics combinations improve the eradication of Helicobacter pylori biofilm in vitro: A comparison with conventional triple therapy.

Antibiotics resistance of H. pylori has been increasing constantly accompanied with decreasing clearance rate clinically, which is demonstrated to be closely related to biofilms with higher resistance than planktonic bacteria for the dense extracellular polymeric substances. Rhamnolipid (RHL) is proved to not only damage the structure of biofilm, but also potentially inhibit bacterial adhesion. To investigate if RHL could promote eradicating rate of the conventional triple therapy to H. pylori biofilm and h...


Growth in the presence of specific antibiotics induces biofilm formation a Campylobacter jejuni strain sensitive to them but not in resistant strains.

Campylobacter jejuni are among the most frequently identified bacteria associated with human gastroenteritis worldwide. Exposure to antibiotics may induce or inhibit biofilm formation in some bacterial species. Little work has been reported on the influence of antibiotics on biofilm formation by C. jejuni.

Occurrence of antibiotics in the main rivers of Shenzhen, China: Association with antibiotic resistance genes and microbial community.

The occurrence and distribution of antibiotics were investigated in surface water and sediment collected from the main rivers of Shenzhen, China. Total concentrations of 20 selected antibiotics ranged from 36.510 to 1075.687 ng L (mean 244.992 ng L) in 31 water samples and from 28.124 to 2728.810 ng g (mean 680.169 ng g) in 31 sediment samples. Notably, STZ and SDZ were the dominant antibiotics in both water and sediment as their higher concentrations compared with the other compounds. Furth...


Response of Anammox biofilm to antibiotics in trace concentration: Microbial activity, diversity and antibiotic resistance genes.

Long-term impacts of two antibiotics-norfloxacin (NOR) and erythromycin (ERY) in trace concentration (1ug L) on Anammox biofilm were investigated. The specific Anammox activity (SAA) and dehydrogenase activity (DHA) of Anammox biofilm were detected by batch experiments, the microbial diversity was analyzed using high-throughput sequencing technology and the antibiotic resistance genes (ARGs) were measured by qPCR. Results showed that long-term NOR feeding decreased 30% SAA and 39.6% DHA, and also decreased ...

An improvised microtiter dish biofilm assay for non-invasive biofilm detection on microbial fuel cell anodes and studying biofilm growth conditions.

Microbial life is predominantly observed as biofilms, which are a sessile aggregation of microbial cells formed in response to stress conditions. The microtiter dish biofilm formation assay is one of the most important methods of studying biofilm formation. In this study, the assay has been improvised to allow easy detection of biofilm formation on different substrata. The method has then been used to study growth conditions that affect biofilm formation, viz., the effect of pH, temperature, shaking conditi...

Simple fluorometric-based assay of antibiotic effectiveness for Acinetobacter baumannii biofilms.

Despite strengthened antimicrobial therapy, biofilm infections of Acinetobacter baumannii are associated with poor prognosis and limited therapeutic options. Assessing antibiotics on planktonic bacteria can result in failure against biofilm infections. Currently, antibiotics to treat biofilm infections are administered empirically, usually without considering the susceptibility of the biofilm objectively before beginning treatment. For effective therapy to resolve biofilm infections it is essential to asses...

Development of methanogens within cathodic biofilm in the single-chamber microbial electrolysis cell.

The aim of this study was to investigate the development of cathodic biofilm and its effect on methane production in a single-chamber microbial electrolysis cell (MEC). The MEC with 1 g/L acetate was successfully operated within 31 cycles (∼2400 h). The maximum methane production rate and average current capture efficiency in the MEC reached 93 L/m·d and 82%, respectively. Distinct stratification of Methanobacteriaceae within cathodic biofilm was observed after 9 cycles of operation. The relative a...

Antimicrobial peptides Pep19-2.5 and Pep19-4LF inhibited Streptococcus mutans growth and biofilm formation.

With this study, we investigated the effect of synthetic antimicrobial peptides Pep19-2.5 and Pep194LF alone or in combination with antibiotics on S. mutans growth and biofilm formation/disruption. We also examined the cytotoxic effect of each peptide on monocytes. S. mutans was cultured in the presence of different concentrations of each peptide. We showed that Pep19-2.5 and Pep19-4LF were able to significantly (p ≤ 0.01) inhibit the growth of S. mutans. The synthetic peptides also decreased biofilm ...

Growth substrate may influence biofilm susceptibility to antibiotics.

The CDC biofilm reactor is a robust culture system with high reproducibility in which biofilms can be grown for a wide variety of analyses. Multiple material types are available as growth substrates, yet data from biofilms grown on biologically relevant materials is scarce, particularly for antibiotic efficacy against differentially supported biofilms. In this study, CDC reactor holders were modified to allow growth of biofilms on collagen, a biologically relevant substrate. Susceptibility to multiple antib...

Development of microbial community within the cathodic biofilm of single-chamber air-cathode microbial fuel cell.

The aim of this study was to investigate the development of microbial community within the cathodic biofilm of single-chamber air-cathode microbial fuel cell (MFC). To analyze microbial community structures within cathodic biofilm, cathodic biofilm samples were stratified into three layers, i.e., the cathode-side layer (0-40 μm), the middle layer (40-80 μm), and the anolyte-side layer (80-120 μm). After four starting cycles (0-188 h), the maximum power densities of the MFC fed with 1 g/L acetat...

Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis.

The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 μg/mL, and the minimum bactericidal concentration (MBC) from 400 to 60...

Microbial Ecology: Functional 'Modules' Drive Assembly of Polysaccharide-Degrading Marine Microbial Communities.

Although ecological principles governing the competition of microbes for simple substrates are well-understood, less is known about how complex, structured substrates influence ecological outcomes in microbial communities. A new study sheds light on how marine microbial communities assemble on polysaccharide particles modeling marine snow.

Reactive oxygen species inhibit biofilm formation of Listeria monocytogenes.

Although the level of reactive oxygen species (ROS) is altered upon the formation of bacterial biofilm, the relationship between ROS alteration and biofilm formation is still unclear. The aim of the present study is to use Listeria monocytogenes (L. monocytogenes) as a model organism to examine whether ROS have an effect on the biofilm formation. After eliminating ROS by treatment with NAD(P)H oxidase inhibitor Diphenyleneiodonium chloride (DPI) or scavenging reagents N-acetylcysteine (NAC), the biofilm for...

Antibiotics Versus No Antibiotics for Acute Uncomplicated Diverticulitis: A Systematic Review and Meta-Analysis.

Antibiotics are routinely used for diverticulitis irrespective of severity. Current practice guidelines favor against the use of antibiotics for acute uncomplicated diverticulitis.

Succession Patterns and Physical Niche Partitioning in Microbial Communities from Subsurface Coal Seams.

The subsurface represents a largely unexplored frontier in microbiology. Here, coal seams present something of an oasis for microbial life, providing moisture, warmth, and abundant fossilized organic material. Microbes in coal seams are thought to syntrophically mobilize fossilized carbon from the geosphere to the biosphere. Despite the environmental and economic importance of this process, little is known about the microbial ecology of coal seams. In the current study, ecological succession and spatial nic...

Effect of Helicobacter pylori biofilm formation on susceptibility to amoxicillin, metronidazole and clarithromycin.

The human gastric pathogen Helicobacter pylori forms biofilms in vitro and in vivo. We previously demonstrated that H. pylori biofilm formation in vitro decreased its susceptibility to clarithromycin (CAM). The aim of this study was to evaluate the effects of biofilm formation on amoxicillin (AMPC) and metronidazole (MNZ) susceptibility. In addition, we assessed the influence of biofilms of CAM resistant H. pylori on CAM susceptibility. It was shown that high levels of efflux pump gene transcripts were dete...

AMPs as Anti-biofilm Agents for Human Therapy and Prophylaxis.

Microbial cells show a strong natural tendency to adhere to surfaces and to colonize them by forming complex communities called biofilms. In this growth mode, biofilm-forming cells encase themselves inside a dense matrix which efficiently protects them against antimicrobial agents and effectors of the immune system. Moreover, at the physiological level, biofilms contain a very heterogeneous cell population including metabolically inactive organisms and persisters, which are highly tolerant to antibiotics. T...

Effect of polyphenol-rich cranberry extracts on cariogenic biofilm properties and microbial composition of polymicrobial biofilms.

To investigate the effect of cranberry extracts on saliva-derived polymicrobial biofilms with regards to biofilm biomass, acidogenicity, exopolysaccharide (EPS)/microbial biovolumes, colony forming unit (CFU) counts, and the relative abundance of specific caries- and health-associated bacteria.

Identification of biofilm formation and exoelectrogenic population structure and function with graphene/polyanliline modified anode in microbial fuel cell.

Improving anode configuration with polymer or nanomaterial modification is promising for enhancing microbial fuel cell performance. However, how anode modification affects biofilm development and electrogenic function remains poorly understood. In this study, the carbon cloth anode modified with polyaniline and reduced graphene oxide was successfully fabricated which obtained the highest power output. Accelerated electrogenic biofilm formation and the better electrogenic bacterial colonization based on the ...

Expression of adhesin genes and biofilm formation among Klebsiella oxytoca clinical isolates from patients with antibiotic-associated haemorrhagic colitis.

Biofilm formation and resistance to last-line antibiotics have restricted chemotherapy options toward infection eradication.

Solution-Mediated Modulation of Biofilm Formation by a Cationic Synthetic Polymer.

Bacterial biofilms and their associated infections are a continuing problem in the healthcare community. Previous approaches utilizing anti-biofilm coatings suffer from short lifetimes, and their applications are limited to surfaces. In this research, we explored a new approach to biofilm prevention based on the hypothesis that changing planktonic bacteria behavior to result in sub-optimal biofilm formation. The behavior of planktonic exposed to a cationic polymer was characterized for changes in growth be...

Sludge anaerobic digestion with high concentrations of tetracyclines and sulfonamides: Dynamics of microbial communities and change of antibiotic resistance genes.

This study established two mesophilic anaerobic digesters to ascertain the microbial dynamics and variation characteristics of antibiotic resistance genes (ARGs) during sludge anaerobic digestion (AD) with high concentration of antibiotics. System parameters, microbial community, ARGs (tetA, tetM, tetW, sulI, sulII) and integrase gene of class 1 (intI1) were analyzed. General performance of AD showed methane production was inhibited by 17.1% under the pressure of antibiotics. Microbial 16S rRNA high-through...

Response of microorganisms in biofilm to sulfadiazine and ciprofloxacin in drinking water distribution systems.

Effects of sulfadiazine and ciprofloxacin on microorganisms in biofilm of drinking water distribution systems (DWDSs) were studied. The results verified that the increases of 16S rRNA for total bacteria and bacterial genus Hyphomicrobium were related to the promotion of antibiotic resistance genes (ARGs) and class 1 integrons (int1) in DWDSs with sulfadiazine and ciprofloxacin. Moreover, the bacteria showed higher enzymatic activities in DWDSs with sulfadiazine and ciprofloxacin, which resulted in more prod...

Fecal Microbial Transplant Capsules are Safe in Hepatic Encephalopathy: A Phase 1, Randomized, Placebo-Controlled Trial.

Hepatic encephalopathy (HE) can cause major morbidity despite standard of care (SOC; rifaximin/lactulose). Fecal microbial transplant (FMT) enemas post-antibiotics are safe but the effect of FMT without antibiotics using the capsular route requires investigation.

Metagenomic Analysis of Zinc Surface-Associated Marine Biofilms.

Biofilms are a significant source of marine biofouling. Marine biofilm communities are established when microorganisms adhere to immersed surfaces. Despite the microbe-inhibiting effect of zinc surfaces, microbes can still attach to the surface and form biofilms. However, the diversity of biofilm-forming microbes that can attach to zinc surfaces and their common functional features remain elusive. Here, by analyzing 9,000,000 16S rRNA gene amplicon sequences and 270 Gb of metagenomic data, we comprehensive...


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