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08:43 EST 21st November 2018 | BioPortfolio

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Showing "Beckman Coulter" PubMed Articles, all 8

Scaling code-multiplexed electrode networks for distributed Coulter detection in microfluidics.

Microfluidic devices can discriminate particles based on their properties and map them into different locations on the device. For distributed detection of these particles, we have recently introduced a multiplexed sensing technique called Microfluidic CODES, which combines code division multiple access with Coulter sensing. Our technique relies on micromachined sensor geometries to produce distinct waveforms that can uniquely be linked to specific locations on the microfluidic device. In this work, we inve...

A very rare interference with the valproate method on the Beckman DxC 800 general chemistry analyser.

Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples.

Nucleic acid material of adequate quality is crucial for successful high-throughput sequencing (HTS) analysis. DNA and RNA isolated from archival FFPE material are frequently degraded and not readily amplifiable due to chemical damage introduced during fixation. To identify optimal nucleic acid extraction kits, DNA and RNA quantity, quality and performance in HTS applications were evaluated. DNA and RNA were isolated from five sarcoma archival FFPE blocks, using eight extraction protocols from seven kits fr...

Falsely high HbA value due to a novel α1-globin gene mutation: Hb shantou α127(H10)Lys > Glu; HBA1: c.382 A > G.

Hemoglobin A (HbA) is a widely utilized biomarker for the diagnosis and management of diabetes mellitus. Here, we describe an α1-globin chain hemoglobin variant and investigate its effect on HbA measurement. A 26-year-old pregnant woman was suspected to harbor a hemoglobin variant following HbA measurement during a routine prenatal examination using D10 (Bio-Rad). An oral glucose tolerance test (OGTT) was performed using an AU5800 clinical chemistry system (Beckman Coulter). HbA was reanalyzed using VII-T ...

Indirect determination of hematology reference intervals in adult patients on Beckman Coulter UniCell DxH 800 and Abbott CELL-DYN Sapphire devices.

Background Conventional establishment of reference intervals for hematological analytes is challenging due to the need to recruit healthy persons. Indirect methods address this by deriving reference intervals from clinical laboratory databases which contain large datasets of both physiological and pathological test results. Methods We used the "Reference Limit Estimator" (RLE) to establish reference intervals for common hematology analytes in adults aged 18-60 years. One hundred and ninety-five samples from...

A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.

We report a high-sensitivity cell secretome detection method using competitive immuno-aggregation and a micro-Coulter counter. A target cell secretome protein competes with anti-biotin-coated microparticles (MPs) to bind with a biotinylated antibody (Ab), causing decreased aggregation of the functionalized MPs and formation of a mixture of MPs and aggregates. In comparison, without the target cell secretome protein, more microparticles are functionalized, and more aggregates are formed. Thus, a decrease in ...

Alpha-fetoprotein in pericardial, peritoneal, and pleural fluids: A body fluid matrix evaluation.

Alpha-fetoprotein (AFP) measurement in pericardial, peritoneal (ascites), and pleural fluids is sometimes requested by clinicians as supportive evidence in the evaluation of suspected malignancy. As commercially available, Food and Drug Administration (FDA)-cleared AFP assays are not validated for these fluid types, laboratories must complete additional validation studies to comply with regulatory requirements for body fluid testing. The objective of this study was therefore to conduct a matrix evaluation f...

Calibration error due to different motion-encoding gradient strength on two scanners.

In this study, displacements of brain tissue in six human subjects and one gelatin "phantom" were measured by MR elastography on two MR scanners. Both were Siemens Trio 3T MRI scanners. One Trio scanner was located at the Beckman Institute at University of Illinois in Urbana-Champaign (UIUC; 4 subjects) and the other Trio scanner was at the Center for Clinical Imaging Research at Washington University in St. Louis (WU; 2 subjects and gel phantom). After publication, we discovered that the default motion-enc...

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