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PubMed Journals Articles About "Collaboration Addresses Downstream Bottlenecks MAbs Production" RSS

02:46 EST 11th December 2019 | BioPortfolio

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Showing "Collaboration addresses downstream bottlenecks mAbs production" PubMed Articles 1–25 of 10,000+

Vector-related stratagems for enhanced monoclonal antibody production in mammalian cells.

Provision of effective monoclonal antibody (mAb) based therapeutics requires careful combination of expression, selection and screening, in a suitable cell culture platform. The cell culture platform, in turn, requires high performance expression vectors for consistent and high production of mAb-based therapeutics. Further, the components of the vector need to be optimized via genetic engineering approaches, to exclude non-productive clones, reduce low secretion levels, eliminate improper functioning that a...


Identification and characterization of co-purifying CHO host cell proteins in monoclonal antibody purification process.

Host cell proteins (HCPs) are process-related impurities derived from the host organism such as Chinese hamster ovary (CHO) cells used for the production of therapeutic mAbs in biopharmaceuticals and potentially pose a risk to patient safety and product efficacy. A number of HCPs have been reported as exceptionally difficult to remove and persist across downstream purification operations into final drug product because they exhibit association with mAbs. Therefore, understanding of HCP impurities and the mA...

A monoclonal antibody recognizing a new epitope on CD81 inhibits T-cell migration without inducing cytokine production.

CD81 is involved in leukocyte migration and cytokine induction. Previous work found that anti-CD81 monoclonal antibodies (mAbs) showed therapeutic potential for several immune diseases via inhibiting leukocyte migration. Although the suppression of cell migration is a promising approach for treating immune diseases, some anti-CD81 mAbs can induce cytokine production, which may exacerbate disease. To obtain new anti-human CD81 mAbs that inhibited migration in the absence of cytokine production enhancement ac...


Human monoclonal antibodies against chikungunya virus target multiple distinct epitopes in the E1 and E2 glycoproteins.

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes persistent arthritis in a subset of human patients. We report the isolation and functional characterization of monoclonal antibodies (mAbs) from two patients infected with CHIKV in the Dominican Republic. Single B cell sorting yielded a panel of 46 human mAbs of diverse germline lineages that targeted epitopes within the E1 or E2 glycoproteins. MAbs that recognized either E1 or E2 proteins exhibited neutralizing activity. Viral escap...

Biosimilar Gene Therapy: Investigational Assessment of Secukinumab Gene Therapy.

Tumor necrosis factor-alpha (TNF-α), checkpoint inhibitors, and interleukin-17 (IL-17) are critical targets in inflammation and autoimmune diseases. Monoclonal antibodies (mAbs) have a successful portfolio in the treatment of chronic diseases. With the current progress in stem cells and gene therapy technologies, there is the promise of replacing costly mAbs production in bioreactors with a more direct and cost-effective production method inside the patient's cells. In this paper we examine the results of ...

Identification of antigenic epitopes in the hemagglutinin protein of H7 avian influenza virus.

The H7 subtype avian influenza virus (AIV) has been reported to infect not only poultry but also humans. The hemagglutinin (HA) protein is the major surface antigen of AIV and plays an important role in viral infection. In this study, five monoclonal antibodies (mAbs) against the HA protein of H7 virus, 2F8, 3F6, 5C11, 5E2 and 5C12, were produced and characterized. Epitope mapping indicated that RESGSS was the minimal linear epitope recognized by the mAbs 2F8/3F6/5C11, and the other mAbs, 5E2/5C12, recogniz...

Human monoclonal antibodies for discovery, therapy, and vaccine acceleration.

Screening of single B cells from convalescent or vaccinated people allows the discovery of novel targets for infectious diseases and rapid production of engineered human monoclonal antibodies (mAbs) that can prevent or control infections by passive immunization. Here we propose that the development of human mAbs can also significantly accelerate vaccine development by anticipating some of the key biological and regulatory questions.

Cathepsin D: removal strategy on Protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production.

Monoclonal antibody (mAb) fragmentation is a well-known degradation pathway that results in product loss and can significantly impact product quality, efficacy, or even cause immunogenic reactions, thus potentially endangering patients' health. It is recognised that residual proteases present among host cell proteins (HCPs) such as those expressed by Chinese Hamster Ovary (CHO) can induce fragmentation, and failure of their complete removal during downstream processing could cause fragmentation during mAb p...

Production of monoclonal antibodies binding to the VP7 protein of African horse sickness virus.

Monoclonal antibodies (MAbs) against AHSV were produced immunising BALB/c mice with AHSV serotype 9 and six clones able to recognize specifically the VP7-AHSV with a strong reactivity were selected. The specificity of the MAbs was assessed in i-ELISA against a commercial VP7-AHSV and in immunoblot against a home-made VP7-AHSV was expressed by a Baculovirus expression system; potential cross-reactions with related orbiviruses (Bluetongue virus and Epizootic Haemorrhagic Disease virus) were investigated as we...

Cuban Scientific Production on Diabetes, 2000-2017: Peer-reviewed Publications, Collaboration and Impact.

INTRODUCTION The steadily increasing prevalence of diabetes globally has captured researchers' attention. Cuban production of scientific articles on diabetes has not been studied from a bibliometric perspective. OBJECTIVE Characterize the production and impact of research and review articles on diabetes by Cuban authors in journals listed in the Scopus bibliographic database, as well as related collaboration among Cuban institutions and between Cuban and non-Cuban institutions. METHODS A bibliometric analys...

Germinality does not necessarily define mAb expression and thermal stability.

The production potential of recombinant monoclonal antibody (mAb) expressing cell lines depends, among other factors, on the intrinsic antibody structure determined by the amino acid sequence. In this study, we investigated the influence of somatic mutations in the V(D)J sequence of four individual, mature model mAbs on the expression potential. Therefore, we defined four couples, each consisting of one naturally occurring mAb (2G12, Ustekinumab, 4B3, and 2F5) and the corresponding germline-derived cognate ...

Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability.

Monoclonal antibodies (mAbs) have recently emerged as one of the most promising classes of biotherapeutics. A potential advantage of B cell-derived mAbs as therapeutic agents is that they have been subjected to natural filtering mechanisms, which may enrich for B cell receptors (BCRs) with favorable biophysical properties. Here, we evaluated 400 human mAbs for polyreactivity, hydrophobicity, and thermal stability using high-throughput screening assays. Overall, mAbs derived from memory B cells and long-live...

Characterization of monoclonal antibodies recognizing each extracellular loop domain of occludin.

The tight junction protein occludin (OCLN) is a four-pass transmembrane protein with two extracellular loops (ELs), and also functions as a co-receptor for hepatitis C virus (HCV). Recently, we reported the establishment of monoclonal antibodies (mAbs) recognizing each intact EL domain of OCLN that can strongly prevent HCV infection in vitro and in vivo and these mAbs were applicable for flow cytometric analysis, immunocytochemistry, and cell-based enzyme-linked immunosorbent assay. In the present study, we...

Forced degradation of monoclonal antibodies after compounding: impact on routine hospital quality control.

Compounded therapeutic monoclonal antibodies (mabs) used in a hospital require quality control (QC). In our hospital, analytical QC process intended to mabs identification and quantification is based on flow injection analysis (FIA) associated with second derivative UV spectroscopy and matching method algorithm. We studied the influence of degraded mabs after compounding on this validated QC. Three forced stress conditions including mechanical, thermal and freeze-thawing stresses were studied to yield degra...

Civilian-military collaboration: From expectations to the reality.

The current global security threats indicate a need for a change in the Swedish defense policies including the role of civilian and military healthcare in an armed conflict. The magnitude, outcome and management of the recent terror and mass casualty incidents in Europe necessitate a closer Swedish civilian-military collaboration. However, in reality, such a collaboration might be more difficult than expected. The aim of this article is to comment on some of the critical points of such collaboration from a ...

Antibody therapy for Lassa fever.

Serum from convalescent Lassa fever patients was previously shown to be ineffective as a source of protective antibodies in some early studies. Subsequently, monoclonal antibodies (MAbs) to the Lassa virus (LASV) glycoprotein produced by memory B cells of West African patients who survived Lassa fever were identified. Development of MAbs as potential Lassa immunotherapeutics was facilitated by structural studies and mutational analyses that identified protective epitopes on the prefusion form of the LASV gl...

Measuring Local Public Health and Primary Care Collaboration: A Practice-Based Research Approach.

To describe the degree of public health and primary care collaboration at the local level and develop a model framework of collaboration, the Community Collaboration Health Model (CCHM).

Microbial production of vitamin K2: current status and future prospects.

Vitamin K2, also called menaquinone, is an essential lipid-soluble vitamin that plays a critical role in blood clotting and prevention of osteoporosis. It has become a focus of research in recent years and has been widely used in the food and pharmaceutical industries. This review will briefly introduce the functions and applications of vitamin K2 first, after which the biosynthesis pathways and enzymes will be analyzed in-depth to highlight the bottlenecks facing the microbial vitamin K2 production on the ...

Rapid Detection of Staphylococcal Enterotoxin-B by Lateral Flow Assay.

A cohort of monoclonal antibodies (mAbs) were generated against Staphylococcal enterotoxin-B (SEB) and selected by double sandwich enzyme-linked immunosorbent assay (ELISA) for solution capture of the toxin. Clonal hybridoma cell lines were established and a pair of anti-SEB mAbs selected for the development of a sandwich ELISA. Immobilized 3D6 mAb (IgG1, kappa) when paired with 4C9 mAb (IgG1, kappa) conjugated to horseradish peroxidase generates a typical dose-response curve with an EC of 24.8 ng/mL for ...

In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered N. benthamiana plants.

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus and its infection can cause long-term debilitating arthritis in humans. Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections. In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model. CHIKV mAb was efficiently expressed and assembled in p...

Unambiguous identification of pyroglutamate in full-length biopharmaceutical monoclonal antibodies by NMR spectroscopy.

Biotherapeutic proteins are an indispensable class of pharmaceuticals that present a high degree of structural complexity and are prone to chemical modifications during production, processing and storage, which have to be tightly controlled. Pyroglutamate (pGlu), a cyclization product of N-terminal Gln or Glu residues, is a widespread post-translational modification in proteins, including monoclonal antibodies (mAbs). The unambiguous identification and quantification of this modification in proteins is chal...

Defining the right diluent for intravenous infusion of therapeutic antibodies.

Therapeutic monoclonal antibodies (mAbs) are commonly administered to patients through intravenous (IV) infusion, which involves diluting the medication into an infusion solution (e.g., saline and 5% dextrose). Using the wrong diluent can cause product aggregation, which may compromise patient safety. We and others have shown that Herceptin® (trastuzumab) and Avastin® (bevacizumab) undergo rapid aggregation upon mixing with dextrose and human plasma in vitro. In this study, we evaluated the compatibility ...

Zinc: A promising material for electrocatalyst-assisted microbial electrosynthesis of carboxylic acids from carbon dioxide.

Microbial electrosynthesis (MES) has been proposed as a sustainable platform to simultaneously achieve wastewater treatment, renewable energy generation and chemicals production. Currently, the CO valorization via MES is restricted by the low production rate, while that via electrochemical reduction is limited by the production of C1 products with high efficiency and selectivity. The electrocatalyst-assisted MES could potentially solve these bottlenecks of both MES and electrochemical reduction technology b...

Monoclonal Antibodies Against Human Papillomavirus E6 and E7 Oncoproteins Inhibit Tumor Growth in Experimental Cervical Cancer.

Nearly all cases of cervical cancer are initiated by persistent infection with high-risk strains of human papillomavirus (hr-HPV). When hr-HPV integrates into the host genome, the constitutive expression of oncogenic HPV proteins E6 and E7 function to disrupt p53 and retinoblastoma regulation of cell cycle, respectively, to favor malignant transformation. HPV E6 and E7 are oncogenes found in over 99% of cervical cancer, they are also expressed in pre-neoplastic stages making these viral oncoproteins attract...

Interprofessional collaboration within fluid teams: community nurses' experiences with palliative home care.

To explore how community nurses (CNs) experience the collaboration with general practitioners (GPs) and specialist palliative home care team (PHCT) nurses in palliative home care and the perceived factors influencing this collaboration.


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