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PubMed Journals Articles About "Eppendorf Research Plus Pipette" RSS

00:49 EST 22nd November 2017 | BioPortfolio

Eppendorf Research Plus Pipette PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Eppendorf Research Plus Pipette articles that have been published worldwide.

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Showing "Eppendorf Research Plus Pipette" PubMed Articles 1–25 of 23,000+

A new scanning mode to improve scanning ion conductance microscopy imaging rate with pipette predicted movement.

Scanning ion conductance microscopy (SICM) is a non-contact surface topography measurement technique that has been increasingly used for soft surfaces such as living biological samples. An approach-retract scanning (ARS) mode is widely used to avoid collision between the SICM probe (i.e., pipette) and an abrupt increase in sample profile. However, the redundant pipette trajectory in the ARS mode lengthens the scan time, thus reducing SICM efficiency and time resolution. To avoid this problem, a new scanning...


Three-Dimensional Super-Resolution Imaging of Single Nanoparticles Delivered by Pipettes.

Controlled three-dimensional positioning of nanoparticles is achieved by delivering single fluorescent nanoparticles from a nanopipette and capturing them at well-defined regions of an electrified substrate. To control the position of single nanoparticles, the force of the pressure-driven flow from the pipette is balanced by the attractive electrostatic force at the substrate, providing a strategy by which nanoparticle trajectories can be manipulated in real time. To visualize nanoparticle motion, a resisti...

A hybrid material as a sorbent phase for the disposable pipette extraction technique enhances efficiency in the determination of phenolic endocrine-disrupting compounds.

In this study, the hybrid material 3-n-propyl(3-methylpyridinium) silsesquioxane chloride (Si3Py(+)Cl(-)) was synthesized and investigated as a novel sorbent phase for the disposable pipette extraction (DPX) technique coupled to high-performance liquid chromatography-florescence detection. This sorbent phase was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Aqueous samples containing the phenolic endocrine-disrupting compounds bisphenol A (BPA), 17α...


Closed-Loop Real-Time Imaging Enables Fully Automated Cell-Targeted Patch-Clamp Neural Recording In Vivo.

Targeted patch-clamp recording is a powerful method for characterizing visually identified cells in intact neural circuits, but it requires skill to perform. We previously developed an algorithm that automates "blind" patching in vivo, but full automation of visually guided, targeted in vivo patching has not been demonstrated, with currently available approaches requiring human intervention to compensate for cell movement as a patch pipette approaches a targeted neuron. Here we present a closed-loop real...

Adding to the Truth of the Matter: The Case for Qualitative Research.

The purpose of this column is to discuss the impact that qualitative research has on translational research, whose aim is to improve the health and well-being of individuals. To that end, rigorous qualitative research is examined; translational research is entertained and the manner in which qualitative research can be a co-equal partner with quantitative research is proposed.

Collaborative Orthopaedic Research Between and Within Institutions.

Collaborative research is common in many medical disciplines; however, the field of orthopaedics has been relatively slow to adopt this type of research approach. Collaborative research efforts can occur between multiple institutions and, in some instances, may benefit from subspecialty society sponsorship. Collaborative research efforts between several research spheres within a single institution also can be advantageous. Collaborative research has many benefits, including a larger number of patients in st...

Modulation of human Kv4.3/KChIP2 channel inactivation kinetics by cytoplasmic Ca(2).

The transient outward current (I to) in the human heart is mediated by Kv4.3 channels complexed with Kv channel interacting protein (KChIP) 2, a cytoplasmic Ca(2+)-binding EF-hand protein known to modulate Kv4.3 inactivation gating upon heterologous co-expression. We studied Kv4.3 channels co-expressed with wild-type (wt) or EF-hand-mutated (ΔEF) KChIP2 in human embryonic kidney (HEK) 293 cells. Co-expression took place in the absence or presence of BAPTA-AM, and macroscopic currents were recorded in the w...

Research Ethics 2.0: New Perspectives on Norms, Values, and Integrity in Genomic Research in Times of Even Scarcer Resources.

Research ethics anew gained importance due to the changing scientific landscape and increasing demands and competition in the academic field. These changes are further exaggerated because of scarce(r) resources in some countries on the one hand and advances in genomics on the other. In this paper, we will highlight the current challenges thereof to scientific integrity. To mark key developments in research ethics, we will distinguish between what we call research ethics 1.0 and research ethics 2.0. Whereas ...

Can research that is not intended or unlikely to be published be considered ethical?

For research to be ethically acceptable, the potential benefits must justify any risks involved for participants. Dissemination of research findings through publication is one way of creating benefit, but not all researchers intend to publish their research. Other factors, such as lack of size or representativeness, generalisability or innovativeness, or negative findings mean the research is unlikely to be published in a peer-reviewed medical journal.

Field Evaluation of Potential Pheromone Lures for Lygus lineolaris (Hemiptera: Miridae) in the Mid-South.

Plant bugs (Hemiptera: Miridae) are phytophagous pests of cultivated plants around the world. In the mid-South region of the United States, Lygus lineolaris (Palisot de Beauvois) is a primary pest of cotton, and causes economic damage. Previously published research about the volatiles produced by members of the genus Lygus, and other closely related groups, indicated that they produce blends of hexyl butyrate, (E)-2-hexenyl butyrate, and (E)-4-oxo-2-hexenal. Varying ratios of the three compounds were loaded...

Clinical Research Nursing: Development of a Residency Program
.

Clinical research nurses are essential in the coordination of clinical trials and the management of research participants. Without a stable, knowledgeable research nurse workforce, the conduct of research is affected. A research nurse residency is a novel approach to preparing new graduate nurses for the oncology research nurse role. This article will describe the development and content of the research nurse residency and how this approach is being used to address a need for clinical research nurses to sup...

Shall Research Ethics Boards be Reviewing Research Methodology!

Electrokinetically controlled fluid injection into unicellular microalgae.

Electrokinetically-controlled microinjection is reported as an effective transport mechanism for microinjection into the wild-type strain of the widely-studied model microalga Chlamydomonas reinhardtii. Microinjection system using glass capillary pipettes was developed to capture and impale the motile cell. To apply an electric field and induce electrokinetic flow (e.g. electrophoresis and electroosmosis), an electrode was inserted directly to the solution inside the impaling injection pipette (IP) and anot...

Foreign Language Short Course: Special Operations Clinical Research Fundamentals.

When we do not know a language, we are at the mercy of an interpreter. The same is true for research: Special Operations Forces (SOF) clinicians not actively involved in research initiatives may rely on scientific interpreters, so it is important to speak some of the language personally. For any clinician, using evidence in practice requires a working knowledge of how that evidence was generated from research, which requires an understanding of research science language. Here we review common basics of rese...

Prioritizing the patient voice in the development of urologic oncology research.

Prioritization of patient and stakeholder engagement in the research process has been realized through increased funding and policy directives at the government level. Particularly, patient engagement in the preparatory research stage has driven development of patient-prioritized research questions. In this article, a successful example of patient-centered research prioritization is reviewed, and effective strategies and opportunities for patient engagement in urologic oncology research are described.

NHS doctors should be more involved in research, says research institute.

Gaps in Current Knowledge and Priorities for Future Research in Dry Eye.

Dry eye, a common yet underrecognized and evolving field, has few recommended treatment algorithms, mostly based on expert consensus rather than robust research evidence. There are high costs associated with managing dry eye and conducting research to identify effective and safe long-term treatments. To support evidence-based management of dry eye, our purpose was to identify and prioritize important clinical research questions for future clinical research.

Family Medicine Research in the United States From the late 1960s Into the Future.

When the new field of family medicine research began a half century ago, multiple individuals and organizations emphasized that research was a key mission. Since the field's inception, there have been notable research successes for which family medicine organizations, researchers, and leaders-assisted by federal and state governments and private foundations-can take credit. Research is a requirement for family medicine residency programs but not individual residents, and multiple family medicine departments...

Show me the money! An analysis of underserved stakeholders' funding priorities in Patient Centered Outcomes Research domains.

Develop an accessible exercise to engage underserved populations about research funding priorities; analyze the criteria they use to prioritize research; contrast these criteria to those currently used by Patient Centered Outcomes Research Institute (PCORI).

Increasing value and reducing waste in stroke research.

Stroke is a major burden to patients and society, and resources spent on stroke research must be used efficiently and produce good value in terms of improvements in human health. However, many instances of poor value from stroke research funding have resulted from the way in which stroke research topics have been chosen and how studies have been designed, conducted, analysed, regulated, managed, disseminated, or reported. A cooperative effort of European stroke researchers aimed to identify sources of ineff...

Patient Preferences May Be Indicative of Normative Issues in Dementia Research.

Robillard and Feng highlight incongruence between patient preferences and the procedural aspects of research ethics as they relate to protocols for dementia research. Their findings break ground for a reassessment of how research ethics, researchers, and participants (including patients and caregivers) approach participation in dementia research. However, it is unclear whether patient preferences may also herald a normative gap between how dementia research is being conducted and how it should be done. This...

Research for us! Which Topics are Relevant for Patients with Depression?

Objective To identify and prioritize research questions about treatment and care of depression which are relevant to patients, carers and clinicians. The importance of involvement of those stakeholder groups in health services research is increasingly recognized internationally. Methods Research topics were collected in an online survey using unipark software. Patients, carers and clinicians wrote down research topics, i. e. uncertainties they experienced when dealing with depression. The stated research ...

Quality evaluation of sperm from livebearing fishes: Standardized assessment of sperm bundles (spermatozeugmata) from Xenotoca eiseni (Goodeidae).

Standardized evaluation of sperm quality is essential for research, commercial-scale cryopreservation, and induced spawning. However, standardized methods for evaluation of sperm bundles (spermatozeugmata or spermatophores) have not been established. The purpose of the present study was to use Redtail Splitfin (Xenotoca eiseni) as a model for freshwater livebearing fishes to establish initial standardized methods to collect sperm bundles, and quantitatively and qualitatively evaluate quality-related attribu...

Robust (and Ethical) Educational Research Designs.

Educational research can be quite confusing to individuals without formal training in educational research methods. Many educators trained in "high-consensus" fields such as the health and medical sciences often expect to utilize the same esteemed experimental and quasi-experimental designs in their educational research efforts, but are surprised to learn that this is often impossible for ethical reasons. This article presents five robust research designs that generally are compatible with education studies...

The Evolving Professional Identity of the Clinical Research Nurse - A Qualitative Exploration.

Clinical research nurses (CRN) make a significant contribution to healthcare research within the UK and internationally. However, lack of clarity about their role, and scope of practice renders their contribution within the profession and in the minds of the wider public invisible. This has implications in terms of promoting the role nurses play not only in terms of recruitment, retention, and care of research participants but also as research leaders of the future.


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