PubMed Journals Articles About "Eppendorf Research Plus Pipette" RSS

21:06 EST 10th December 2018 | BioPortfolio

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Showing "Eppendorf Research Plus Pipette" PubMed Articles 1–25 of 23,000+

Development of an On-Chip Antibiotic Permeability Assay With Single Molecule Detection Capability.

Electrophysiology is the method of choice to characterize membrane channels. In this paper, we demonstrate a patch pipette based simple miniaturization that allows performing conductance measurements on a planar lipid bilayer in a microfluidic channel. Membrane proteins were reconstituted into Giant Unilamellar Vesicles (GUVs) by electroswelling, and GUVs with a single channel insertion were patched at the tip of pipette. We applied this approach to investigate the interactions of porins from E.coli with si...

An effective and high-throughput analytical methodology for pesticide screening in human urine by disposable pipette extraction and gas chromatography - mass spectrometry.

In this paper, the use of disposable pipette extraction (DPX) for the determination of pesticides in human urine in possible cases of poisoning is proposed for the first time. The pesticides studied were oxamyl, propoxur, carbofuran, 3‑hydroxycarbofuran, carbaryl, methiocarb, terbufos, parathion methyl, malathion, chlorpyrifos and endosulfan. The pipette tip used for the extraction of these compounds was commercially acquired. It has a capacity of 5 mL and contains 20 mg of sorbent material (styrene-d...

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette-tip solid-phase extraction for the determination of antiretrovirals from plasma of HIV-infected patients.

In this work a hollow mesoporous structured molecularly imprinted polymer was synthetized and used as adsorbent in pipette-tip solid-phase extraction for the determination of lamivudine (3TC), zidovudine (AZT) and efavirenz (EFZ) from plasma of human immunodeficiency virus (HIV) infected patients by high-performance liquid chromatography (HPLC). All parameters that influence the recovery of the pipette tip based on hollow mesoporous molecularly imprinted polymer solid-phase extraction (PT-HM-MIP-SPE) method...

Preparation of acryloyl β-cyclodextrin-silica hybrid monolithic column and its application in pipette tip solid-phase extraction and HPLC analysis of methyl parathion and fenthion.

An acryloyl β-cyclodextrin-silica hybrid monolithic column for pipette tip solid-phase extraction and high-performance liquid chromatography determination of methyl parathion and fenthion have been prepared through a sol-gel polymerization method. The synthesis conditions, including the volume of cross-linker and the ratio of inorganic solution to organic solution, were optimized. The prepared monolithic column was characterized by thermogravimetric analysis, scanning electron microscopy and Fourier transf...

Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction.

The first step to perceive molecules in the air as odors is their detection by the olfactory receptors (ORs) present in the cilia of the olfactory sensory neurons (OSNs) in the nasal cavity. The binding of the odorant molecule to the OR triggers a series of biochemical events that lead to the opening of ion channels, creating at first a generator potential that, if the latter reaches threshold, leads to action potential firing. New insights into olfactory transduction introduced new key players and highligh...

Increasing Residency Research Output While Cultivating Community Research Collaborations.

Having a research curriculum in addition to hosting a resident research day stimulates research activity in residency programs. Research collaborations outside an individual residency program may also promote research in residency. This paper describes a community-wide health research forum that engages faculty and residents in research while bringing together potential research collaborators from the community.

Resistive Analysis of Hydrogen Peroxide in one Axon of single Neuron with Nano-pipettes.

Electrochemical analysis of intracellular hydrogen peroxide in sub-micron scaled cellular protrusion is challenging that requires highly sensitive nanoelectrode and advanced electrochemical detection system. In this technical note, a resistive analysis based on acrylic acid polymerization in the nano-pipettes is established to measure hydrogen peroxide in one axon of single neuron. Upon the position of nano-pipette tip inside the axon, hydrogen peroxide is electrokinetically loaded into the pipette to react...

Application of a sperm survival test: Results from an external quality control programme.

The study aim is to determine which type of material - pipette tips or culture medium - is more appropriate for use in a cytotoxicity external quality control programme (CT-EQC).

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy.

Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional microscopy to surface nephron features, and 2-photon studies are limited in that interventions can only be assessed at the organ, rather than the nephron level. In particular, micropuncture studies of the glomeruli of mice have been challenged by the paucity of surface glomeruli in mice. To address this limitation in order to pursue studies of aspirate fr...

Cultivating the Human Dimension in Research.

According to "Research Exemplars," research requires attending to matters of heart as much as mind. The human dimension in research-relationships, passion, resilience, and leadership-was the common thread in their advice for a successful career. We discuss strategies to cultivate intra- and inter-personal skills fostering these aspects of research.

Development and validation of the Person-Centeredness of Research Scale.

Person-centeredness shifts the focus of healthcare and research to the needs and priorities of patients and communities, and may improve health outcomes. There are no instruments available, however, with which we can assess the degree to which research is indeed person-centered. Our aim was to develop and validate a quantitative instrument to rate person-centeredness of research.

Evaluation of a Center of Research Excellence in Polycystic Ovary Syndrome as a Large-Scale Collaborative Research Translation Initiative, Including Evaluating Translation of Guideline Impact.

Research translation and evaluation are often underconsidered in research design and implementation thus limiting research benefit to the end user. In this article, we first describe a best practice approach to evaluation, for a center of research excellence in polycystic ovary syndrome. Within this, we outline a comprehensive research translation program with inbuilt evaluation of the first (2018). We seek to provide a real-world example of comprehensive approaches to evaluation and research translation.

Optimization of spider silk sphere formation processing conditions to obtain carriers with controlled characteristics.

Bioengineered spider silk is a focus of research due to its biocompatibility, biodegradability, and excellent mechanical properties. Functionalized silk can be processed into spheres and employed as selective drug delivery vehicles in targeted cancer treatment. Efficient, repeatable and controllable processing conditions are essential to drug carrier development. This study aimed to optimize the processing conditions of silk spheres formation, scale-up, and automation of the silk spheres production process....

A bibliometric analysis of tuberculosis research, 2007-2016.

Tuberculosis (TB) research is a key component of the End TB Strategy. To track research output, we conducted a bibliometric analysis of TB research from the past decade.

Global research partnerships in advancing public health: A case study on India.

Collaborative research is integral to medicine. Multi-national and multi-institutional research partnerships produce advances in medicine and public health that have a significant societal impact. Developing nations can gain from such collaborative partnerships in achieving progress in sustainable development goals. However, it is important that the research agenda is relevant to the region where studies are conducted. Funding of research by the national government and regional organisations will ensure tha...

Capacity to conduct health research among NGOs in Malawi: Diverse strengths, needs and opportunities for development.

The role of non-governmental organisations (NGOs) in health research has attracted growing attention. NGOs are important service providers and advocates in international health, and conducting research can help NGOs to strengthen these service delivery and advocacy activities. However, capacity to conduct research varies among NGOs. There is currently limited evidence on NGOs' research capacity that can explain why capacity varies or indicate potential areas for support. We examined NGOs' capacity to conduc...

Evaluation of Apically Extruded Debris Using Continuous Rotation, Reciprocation, or Adaptive Motion.

The aim of this study was to compare the amount of apically extruded debris during root canal instrumentation using ProTaper Next (PTN), Twisted File (TF) Adaptive, and Reciproc instruments. Forty-five extracted human maxillary canines were selected and randomly assigned into 3 groups. The root canals were prepared using PTN instruments with continuous rotation (n=15), TF Adaptive instruments with adaptive motion (n=15), Reciproc instruments with reciprocating motion (n=15). During the preparations, canals ...

Is It Time to Re-Evaluate the Ethics Governance of Social Media Research?

This article reports on a U.K. workshop on social media research ethics held in May 2018. There were 10 expert speakers and an audience of researchers, research ethics committee members, and research institution representatives. Participants reviewed the current state of social media ethics, discussing well-rehearsed questions such as what needs consent in social media research, and how the public/private divide differs between virtual and real-life environments. The lack of answers to such questions was no...

Superhydrophobic candle soot/PDMS substrate for one-step enrichment and desalting of peptides in MALDI MS analysis.

Superhydrophobic substrate is applied in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) detection due to its confinement effect. The weak interaction of superhydrophobic surface with water/salts makes it potential in one-step enrichment and desalting of peptide in MALDI MS analysis. We fabricate a superhydrophobic substrate by spin-coating poly(dimethyl siloxane) (PDMS) on a candle soot layer. On this substrate, the peptide analytes can be confined and enriched in a small area due ...

Commentary: Paradigms, Axiology, and Praxeology in Medical Education Research.

This supplement includes 11 research papers accepted by the 2018 Research in Medical Education Committee. In this Commentary, the authors draw the attention of medical educators and researchers to questions about the researcher's philosophical paradigm. Such questions fundamentally guide research-the choice of conceptual frameworks, methods, and methodology. The authors provide a brief overview of research paradigms and the related concept of axiology and praxeology in medical education research. The author...

What is real success in nursing research?

Who and what can define what success is in research? With the United Kingdom's seven-yearly Research Excellence Framework and recent anniversaries such as the 40 of the Journal of Advanced Nursing providing an opportunity to look back and assess progress made and successes achieved. But how much do we do this as individual researchers?. Research success in nursing occurs mostly in a small disciplinary goldfish bowl. While the profession has progressed in terms of the number of journals, professors and volum...

Health Research Participation, Opportunity, and Willingness Among Minority and Rural Communities of Arkansas.

Prior research suggests that rural and minority communities participate in research at lower rates. While rural and minority populations are often cited as being underrepresented in research, population-based studies on health research participation have not been conducted. This study used questions added to the 2015 Behavioral Risk Factor Surveillance System to understand factors associated with i) health research participation, ii) opportunities to participate in health research, and iii) willingness to p...

"Electronic Cigarettes" are not Cigarettes, and why that Matters.

With research about electronic cigarettes (e-cigarettes) rapidly increasing, this commentary addresses the conceptualization of e-cigarettes as similar to traditional cigarettes. The more we attempt to understand and measure e-cigarettes as equivalent to cigarettes, the more likely research may err in conclusions about these unique devices. Our commentary notes how using unique conceptualizations and measures for e-cigarettes will help accelerate new research.

A Novel Stakeholder Engagement Approach for Patient-centered Outcomes Research.

The engagement of patients and other stakeholders is a critical element in the design of patient-centered outcomes research studies. However, methodology for scalable engagement in research management particularly activities such as operationalization of principles and setting of priorities is not well-developed. The objective of this study is to describe a novel approach for scalable stakeholder engagement in research aligned with the Patient-Centered Outcomes Research Institute (PCORI) engagement principl...

Knowledge And Attitude Of Undergraduate Dental Students Towards Research.

Scientific research is an important part of healthcare curricula and a vital exercise to help develop clinician's research skills. The aim of this study was to evaluate quantitatively the knowledge and attitude of undergraduate dental students towards scientific research. In addition, the study explored the reasons for lack of interest in research by the dental students.

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