Topics

PubMed Journals Articles About "Inside Lipid Droplets" RSS

03:46 EST 27th February 2020 | BioPortfolio

Inside Lipid Droplets PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Inside Lipid Droplets articles that have been published worldwide.

More Information about "Inside Lipid Droplets" on BioPortfolio

We have published hundreds of Inside Lipid Droplets news stories on BioPortfolio along with dozens of Inside Lipid Droplets Clinical Trials and PubMed Articles about Inside Lipid Droplets for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Inside Lipid Droplets Companies in our database. You can also find out about relevant Inside Lipid Droplets Drugs and Medications on this site too.

Showing "Inside Lipid Droplets" PubMed Articles 1–25 of 4,300+

Targeting PLIN2/PLIN5-PPARγ: SFN Disturbs the Maturation of Lipid Droplets.

The effects of sulforaphane (SFN) on the maturation of lipid droplets (LDs)-the storage units for free fatty acids and sterols as triacylglycerides (TAG) and cholesterol esters (CE)-is far from understood, despite the fact that SFN is known to be beneficial for ameliorating lipid metabolism disorders.


Modulation of Physicochemical Characteristics of Pickering Emulsions: Utilization of Nanocellulose- and Nanochitin-coated Lipid Droplet Blends.

Mixed Pickering emulsions were prepared by blending anionic nanocellulose-stabilized lipid droplets with cationic nanochitin-stabilized lipid droplets. Changes in the surface potential, particle size, shear viscosity, and morphology of the mixed emulsions were characterized when the droplet mixing ratio was varied. Emulsion properties could be tailored by altering the pH and mixing ratio. Surface potential measurements suggested that the nanochitin-coated lipid droplets adsorbed to the surfaces of the nanoc...

A Twist of FATe: Lipid Droplets and Inflammatory Lipid Mediators.

Lipid droplets are fat storage organelles present in most eukaryotic cells. They consist of a neutral lipid core containing mostly triglycerides and sterol esters and covered by a monolayer of phospholipids, wherein numerous proteins are embedded. In the cell, lipid droplets have a dynamic life cycle, rapidly altering their size, location, lipid and protein composition in response to environmental stimuli and cell state. Lipid droplets are primarily involved in the coordination of lipid metabolism with cell...


Lipid Droplets Grease Enterovirus Replication.

Replication complexes of (+)RNA viruses of eukaryotes are associated with specialized membranous domains, termed replication organelles. How these structures develop is poorly understood. In a recent Cell paper, Laufman et al. (2019) reveal that enteroviruses recruit lipid droplets to support lipid synthesis required for the structural development of replication organelles.

Application of flow cytometry as novel technology in studying the effect of droplet size on lipid oxidation in oil-in-water emulsions.

Despite several published studies, the impact of emulsion droplet size on lipid oxidation rates is unclear. This could be because oil-in-water emulsions are typically polydisperse and the oxidation rate of individual droplets is difficult to discern. Flow cytometry is a technique for studying individual cells and their subpopulations using fluorescence technologies, which is possible to be used in studying individual emulsion droplets. Typical emulsion droplets are too small to be visualized by flow cytomet...

Manipulating and studying triglyceride droplets in microfluidic devices.

Triglyceride is the main lipid class in nature, found as droplets in both living systems and man-made products (such as manufactured foods and drugs). Characterizing triglyceride droplets in situ in these systems is complex due to many environmental interactions. To answer basic research questions about droplet formation, structuration, stability, or degradation, microfluidic strategies were developed, allowing well-controlled droplets to be formed, manipulated, and studied. In this review, these strategies...

Multiple cellular pathways regulate lipid droplet homeostasis for the establishment of polarity in collagen sandwich-cultured hepatocytes.

Hepatocyte polarization is energy dependent. The establishment of polarization in collagen sandwich culture of hepatocytes requires utilization of lipid droplets and mitochondrial β-oxidation to supply ATPs. Multiple cellular pathways are involved in lipid droplet homeostasis; however, mechanistic insights of how hepatocytes utilize lipid droplets during polarization remain elusive. The current study investigated the effects of various pathways involved in lipid droplet homeostasis on bioenergetics during ...

Functional diversity of glycerolipid acylhydrolases in plant metabolism and physiology.

Most current knowledge about plant lipid metabolism has focused on the biosynthesis of lipids and their transport between different organelles. However, lipid composition changes during development and in response to environmental cues often go beyond adjustments of lipid biosynthesis. When lipids have to be removed to adjust the extent of membranes during down regulation of photosynthesis, or lipid composition has to be adjusted to alter the biophysical properties of membranes, or lipid derived chemical si...

LDAF1 Holds the Key to Seipin Function.

Seipin is an ER protein important for the assembly of cytoplasmic lipid droplets. In this issue of Developmental Cell, Chung et al. (2019) show that a stable seipin-binding protein, LDAF1/promethin, functions with seipin by attracting triacylglycerol and then allowing this lipid to accumulate and partition into nascent droplets.

Triphenylamine Schiff base as a lipid droplet-targeted fluorescent probe using Si-O-Si as a bridge for the detection of Cr applied in bio-imaging.

Lipid droplets are known to play an important role in many cellular activities, as revealed by recent studies. Additionally, hexavalent chromium is considered extremely toxic because it readily passes through cellular membranes and easily accumulates in living cells. In this study, a novel lipid droplet-targeted fluorescent probe (Si-LDS) for recognition of Cr6+ in living cells was designed and synthesized using triphenylamine derivatives and organosiloxane. Si-LDS detected Cr6+ with high selectivity and se...

A new insight into the mechanism for cytosolic lipid droplet degradation in senescent leaves.

Leaf senescence involves lipid droplet degradation that produces toxic fatty acids, but little is known about how the toxic metabolites are isolated from the rest of the cellular components. Our ultramicroscopic characterization of cytosolic lipid droplet degradation in central vacuole-absent cells and central vacuole-containing cells of senescent watermelon leaves demonstrated two degradation pathways: the small vacuole-associated pathway and the central vacuole-associated pathway. This provided an insight...

Application of flow cytometry as novel technology in studying lipid oxidation and mass transport phenomena in oil-in-water emulsions.

Flow cytometry was used to determine if lipid oxidation products could transfer between individual emulsion droplets. Medium chain triacyclglycerol oil-in-water emulsions containing an oxidizable fluorescent dye, BODIPY, was blended with a soybean oil-in-water emulsion. Results showed that when the concentration of sodium dodecyl sulfonate (SDS) were lower than critical micelle concentration (CMC), lipid oxidation products of triacylglycerols were not able to escape out until emulsions were extremely oxidiz...

Photopolymerized microdomains in both lipid leaflets establish diffusive transport pathways across biomimetic membranes.

Controlled transport within a network of aqueous subcompartments provides a foundation for the construction of biologically-inspired materials. These materials are commonly assembled using the droplet interface bilayer (DIB) technique, adhering droplets together into a network of lipid membranes. DIB structures may be functionalized to generate conductive pathways by enhancing the permeability of pre-selected membranes, a strategy inspired by nature. Traditionally these pathways are generated by dissolving ...

TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh.

Milk lipid secretion is a critical process for the delivery of nutrition and energy from parent to offspring. However, the underlying molecular mechanism is less clear. Here we report that TDP-43, a RNA-binding protein, underwent positive selection in the mammalian lineage. Furthermore, TDP-43 gene (Tardbp) loss induces accumulation of large lipid droplets and severe lipid secretion deficiency in mammary epithelial cells to outside alveolar lumens, eventually resulting in lactation failure and pup starvatio...

Coexistence of Oil Droplets and Lipid Vesicles in Propofol Drug Products.

Propofol is intravenously administered oil-in-water emulsion stabilized by egg lecithin phospholipids indicated for the induction and maintenance of general anesthesia or sedation. It is generally assumed to be structurally homogenous as characterized by commonly used dynamic light scattering technique and laser diffraction. However, the excessive amount of egg lecithin phospholipids added to the propofol formulation may, presumably, give rise to additional formation of lipid vesicles (i.e., vesicular struc...

Evaluating adipocyte differentiation of bone marrow-derived mesenchymal stem cells by a deep learning method for automatic lipid droplet counting.

Stem cells are a group of competent cells capable of self-renewal and differentiating into osteogenic, chondrogenic, and adipogenic lineages. These cells provide the possibility of successfully treating patients. During differentiation into adipose tissues, a large number of lipid droplets normally accumulate in these cells, which can be seen through oil red O staining. Although the oil red O staining technique is regularly used for assessing the differentiation degree, its validity for quantitative studies...

Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression.

Lipid droplets (LDs) store lipids for energy and are central to cellular lipid homeostasis. The mechanisms coordinating lipid storage in LDs with cellular metabolism are unclear but relevant to obesity-related diseases. Here we utilized genome-wide screening to identify genes that modulate lipid storage in macrophages, a cell type involved in metabolic diseases. Among ∼550 identified screen hits is MLX, a basic helix-loop-helix leucine-zipper transcription factor that regulates metabolic processes. We sho...

Clinicopathological features of hepatocellular carcinoma with fatty change: Tumors with macrovesicular steatosis have better prognosis and aberrant expression patterns of perilipin and adipophilin.

The clinicopathological characteristics of steatosis in hepatocellular carcinoma (HCC) remain unclear. Here, we elucidate the features of macrovesicular steatosis (MaS) and microvesicular steatosis (MiS) in HCC and their relationships with background liver steatosis. A total of 165 HCC lesions were classified as MaS-HCC, MiS-HCC, or conventional HCC (cHCC) according to the cutoff value of 30% MaS or MiS in tumor cells. We analyzed the clinicopathological differences among these groups. MaS-HCC had less port...

"Basicles": Microbial growth and production monitoring in giant lipid vesicles.

We present a method and protocol to encapsulate bacteria inside giant unilamellar lipid vesicles combined with a microfluidic platform for real-time monitoring of microbial growth and production. The microfluidic device allows to immobilize the lipid vesicles, and record bacterial growth and production using automated microscopy. Moreover, the lipid vesicles retain hydrophilic molecules and therefore, can be used to accumulate products of microbial biosynthesis, which we demonstrate here for a riboflavin-pr...

Telmisartan prevents development of obesity and normalizes hypothalamic lipid droplets.

The AT1 receptor blocker telmisartan (TEL) prevents diet-induced obesity. Hypothalamic lipid metabolism is functionally important for energy homeostasis, as a surplus of lipids induces an inflammatory response in the hypothalamus, thus promoting the development of central leptin resistance. However, it is unclear as to whether TEL treatment affects the lipid status in the hypothalamus.C57BL/6N mice were fed with chow (CONchow) or high-fat diet (CONHFD). HFD-fed mice were gavaged with TEL (8 mg/kg/d, 12 week...

The biogenesis of lipid droplets: Lipids take center stage.

Lipid droplets (LDs) are multi-functional cellular organelles that store energy, and regulate many aspects of cell physiology. However, our understanding of the biogenesis of LDs remains very limited. Originating from the endoplasmic reticulum (ER), LDs are highly unique organelles in that each LD is bounded by a monolayer of amphipathic lipids. Recent progress has unveiled critical roles of non-bilayer lipids in LD formation. For instance, non-bilayer lipids such as lysophospholipids, diacylglycerol and ph...

RAB18 loss interferes with lipid droplet catabolism and provokes autophagy network adaptations.

Autophagy is dependent on appropriate lipid supply for autophagosome formation. The regulation of lipid acquisition and the autophagy network response to lipid-limiting conditions are mostly elusive. Here, we show that the knockout of the RAB GTPase RAB18 interferes with lipid droplet catabolism, causing an impaired fatty acid release. The resulting reduced lipid droplet-derived lipid availability influences autophagy and provokes adaptive modifications of the autophagy network. These adjustments include in...

Formation, characterization, and application of chitosan/pectin-stabilized multilayer emulsions as astaxanthin delivery systems.

Multilayer emulsions were formed by sequential electrostatic deposition of anionic (pectin) and cationic (chitosan) biopolymers onto anionic saponin-coated lipid droplets. These emulsions were then tested for their ability to encapsulate and protect a hydrophobic nutraceutical (astaxanthin). The impact of chitosan and pectin concentration, pH, and ionic strength on the formation and stability of the multilayer emulsions was examined. Multilayer emulsions containing small uniform particles were produced usin...

Special issue "Biogenesis and Fate of Lipid Droplets".

Hydrodynamic and physicochemical phenomena in liquid droplets under the action of nanosecond spark discharges: A review.

This review presents experimental studies of phenomena occurring in droplets of various liquids under the effect of nanosecond spark discharges. Inorganic liquids and liquids of biological origin are considered here. Attention is payed to hydrodynamic and physico-chemical phenomena in droplets, including a movement of sessile droplets on a substrate under the effect of the discharges, internal flows in droplets excited by the discharges, plasma capillary phenomena, features of the droplets drying under the ...


Quick Search